A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}EP1174EP1174 Insertion

General Information
Symbol Dmel\P{EP}EP1174EP1174 Species D. melanogaster
Name FlyBase ID FBti0011835
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) EP1174, PsGEF Viability / fertility
Causes allele(s) EP1174EP1174 Stock availability 1 publicly available
LINE ID EP(X)1174
Genomic Location
Chromosomal location X ( 3A2 ) Sequence location X:2,281,801..2,281,801 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0053079 FBti0068115 FBti0072011 FBti0052957 FBti0033572 FBti0034170 FBti0037273 FBti0020928 FBti0035322 FBti0068709 FBti0035552 FBti0037351 FBti0056387 FBti0054467 FBti0036372 FBti0028830 FBti0011835 FBti0035261 FBti0111853 FBti0071843
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
X:2,281,801..2,281,801 [-]
(FlyBase, 2005)
Orientation
Cytological location
(computed by FlyBase)
3A2 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
2F5-2F5 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
3A1-3A2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ254760
(Benos et al., 2001, FlyBase, 1992-)
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
EP1174
(Jones et al., 2009)
PsGEF
(BDGP Project Members, 2000-, Benos et al., 2001, Jones et al., 2009)
hide Alleles and Phenotypes
Causes alleles
EP1174EP1174
(Jones et al., 2009)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Age-related locomotor impairment is significantly delayed in mutant males compared to controls. Mutant males show increased resistance to paraquat compared to controls.
(Jones et al., 2009)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(X)1174
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
10991
P{EP}EP1174EP1174 w1118
hide Linkouts
hide Comments
Location X:2243923-2243924 determined by FlyBase alignment of dbGSS accession AQ254760 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
Maps to clone: BACH7M4
(Benos et al., 2001)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EP1174
(Jones et al., 2009)
EP(1)1174
(Benos et al., 2001)
EP(X)1174
(FlyBase, 1992-)
P{EP}EP1174
(FlyBase, 2005, FlyBase, 1992-)
P{EP}EP1174EP1174
 
Secondary FlyBase IDs
hide References ( 10 )
Research paper
Jones et al., 2009, Exp. Gerontol. 44(8): 532--540
A forward genetic screen in Drosophila implicates insulin signaling in age-related locomotor impairment. [FBrf0208462]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Benos et al., 2001, Genome Res. 11(5): 710--730
From First Base: the sequence of the tip of the X chromosome of Drosophila melanogaster, a comparison of two sequencing strategies. [FBrf0135823]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]