A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}Hmt4-20EP1216a Insertion

General Information
Symbol Dmel\P{EP}Hmt4-20EP1216a Species D. melanogaster
Name FlyBase ID FBti0011838
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) Hmt4-20, skpA Viability / fertility
Causes allele(s) Hmt4-20EP1216a, skpAEP1216a Stock availability none publicly available
LINE ID EP(X)1216
Genomic Location
Chromosomal location X ( 1B13 ) Sequence location X:548,271..548,271 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0063323 FBti0040187 FBti0114780 FBti0018384 FBti0011838 FBti0151491 FBti0049798 FBti0039449 FBti0055535 FBti0023358 FBti0034590 FBti0029703 FBti0012828 FBti0015685 FBti0071585 FBti0012840 FBti0029208 FBti0007441 FBti0071247 FBti0052408 FBti0049038 FBti0023210
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
What does this section display?
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What does this section not display?
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
X:548,271..548,271 [+]
(BDGP Project Members, 2000-)
Orientation
Cytological location
(computed by FlyBase)
1B13 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
1B7-1B7 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
1B13--1B14 (in situ hybridization reported)
(Abdelilah-Seyfried et al., 2000)
1B13-1B14 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ254762
(Benos et al., 2001, FlyBase, 1992-)
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Hmt4-20
(BDGP Project Members, 2000-, Benos et al., 2001, Sakaguchi et al., 2008)
skpA
(Wing et al., 2002, Zhu et al., 2005, FlyBase, 1996-)
hide Alleles and Phenotypes
Causes alleles
Hmt4-20EP1216a
(FlyBase, 1996-, Zhu et al., 2005, Sakaguchi et al., 2008)
skpAEP1216a
(Wing et al., 2002, Zhu et al., 2005, FlyBase, 1996-)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
The eye phenotype caused by expression of grim::rprrg.Scer\UAS under the control of Scer\GAL4GMR.PF is suppressed by the "EP1216" line, which contains two P{EP} insertions; P{EP}Suv4-20EP1216a and P{EP}EP1216bEP1216b.
(Wing et al., 2002)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(X)1216
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
t
(Gene Disruption Project members, 2001-)
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
Location X:511059-511060 confirmed by FlyBase alignment of dbGSS accession AQ254762 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
Maps to clone: 115C2
(Benos et al., 2001)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EP(1)1216
(Benos et al., 2001)
EP(X)1216
(FlyBase, 1992-)
EP(X)1216a
(Abdelilah-Seyfried et al., 2000, Noureddine et al., 2002)
P{EP}EP1216
(FlyBase, 1992-)
P{EP}EP1216a
(FlyBase, 1992-)
P{EP}Hmt4-20EP1216a
 
P{EP}skpAEP1216a
(FlyBase, 1992-)
P{EP}Suv4-20EP1216a
(FlyBase, 2005, FlyBase, 1992-)
PEP1216a
(Sakaguchi et al., 2008)
Secondary FlyBase IDs
hide References ( 15 )
Research paper
Sakaguchi et al., 2008, Genetics 179(1): 317--322
Functional characterization of the Drosophila Hmt4-20/Suv4-20 histone methyltransferase. [FBrf0204822]
Zhu et al., 2005, Genetics 170(2): 767--777
A screen for genes that influence fibroblast growth factor signal transduction in Drosophila. [FBrf0187664]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Noureddine et al., 2002, Dev. Cell 2(6): 757--770
Drosophila Roc1a encodes a RING-H2 protein with a unique function in processing the Hh signal transducer Ci by the SCF E3 ubiquitin ligase. [FBrf0148948]
Wing et al., 2002, Nat. Cell Biol. 4(6): 451--456
Drosophila Morgue is an F box/ubiquitin conjugase domain protein important for grim-reaper mediated apoptosis. [FBrf0149135]
Benos et al., 2001, Genome Res. 11(5): 710--730
From First Base: the sequence of the tip of the X chromosome of Drosophila melanogaster, a comparison of two sequencing strategies. [FBrf0135823]
Abdelilah-Seyfried et al., 2000, Genetics 155(2): 733--752
A gain-of-function screen for genes that affect the development of the Drosophila adult external sensory organ. [FBrf0128367]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1996-, FlyBase inference based on genome sequence analysis.
FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]