Dmel\P{EP}Hr4^EP1232 Insertion

General Information
Symbol	Dmel\P{EP}Hr4^EP1232	Species	D. melanogaster
Name		FlyBase ID	FBti0011842
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	Hr4	Viability / fertility
Causes allele(s)	Hr4^EP1232	Stock availability	1 publicly available
LINE ID	EP(X)1232
Genomic Location
Chromosomal location	X ( 2B17 )	Sequence location	X:1,845,712..1,845,712 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	X:1,845,712..1,845,712 [+] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	2B17 ( inferred by FlyBase from sequence location )
Cytological location (reported)	2A4-2A4 (reported as inferred from sequence location) (BDGP Project Members, 2000-) 2B16-2B18 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ254763 (Benos et al., 2001, FlyBase, 1992-)
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Hr4 (Gene Disruption Project members, 2001-, Tseng and Hariharan, 2002, Pena-Rangel et al., 2002, Mueller et al., 2005, Abdelilah-Seyfried et al., 2000)
Alleles and Phenotypes
Causes alleles	Hr4^EP1232 (Abdelilah-Seyfried et al., 2000, Mueller et al., 2005, Pena-Rangel et al., 2002, Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-)

Lethality References partially lethal - majority die (Tseng and Hariharan, 2002)
Sterility References
Phenotype Manifest In
	dorsocentral bristle (Abdelilah-Seyfried et al., 2000) eye (Tseng and Hariharan, 2002, Mueller et al., 2005) ommatidium (Tseng and Hariharan, 2002) scutellar bristle (Abdelilah-Seyfried et al., 2000) tormogen cell (Abdelilah-Seyfried et al., 2000) wing (Tseng and Hariharan, 2002)
Detailed Description
Statement Reference Expression of CG3600^EP1232 under the control of Scer\GAL4^GMR.PF results in large, rough eyes. (Mueller et al., 2005) pnr^MD237/+ adults expressing CG3600^EP1232 under the control of Scer\GAL4^pnr-MD237 show loss of some macrochaetae and microchaetae. (Pena-Rangel et al., 2002) Co-expression of CycE^Scer\UAS.cLa does not suppress the small eye phenotype caused by expression of CG3600^EP1232 under the control of Scer\GAL4^ey.PH. (Tseng and Hariharan, 2002) Expression of CG3600^EP1232 under the control of Scer\GAL4^ey.PH results in eyes that are somewhat reduced in size. Flies expressing CG3600^EP1232 under the control of Scer\GAL4^hs.2sev have rough, slightly glassy eyes. Flies expressing CG3600^EP1232 under the control of Scer\GAL4^en-e16E have folds in the wing. (Tseng and Hariharan, 2002) Expression of CG3600^EP1232 under the control of Scer\GAL4^sca-537.4 results in the loss of external support cells of a few scutellar and dorsocentral macrochaetae without loss of the sheath cell. Sensory organs with no socket and one shaft are seen. (Abdelilah-Seyfried et al., 2000)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(X)1232 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Kyoto	122041 w¹¹¹⁸ P{EP}Hr4^EP1232
Linkouts
Comments
	Location X:1807835-1807836 confirmed by FlyBase alignment of dbGSS accession AQ254763 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) Maps to clone: 39E1 (Benos et al., 2001) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP(1)1232 (Benos et al., 2001) P{EP}CG3600^EP1232 (FlyBase, 1992-) P{EP}EP1232 (FlyBase, 1992-) P{EP}EP1232^EP1232 (FlyBase, 1992-, FlyBase, 2005) P{EP}Hr4^EP1232
Secondary FlyBase IDs

References ( 13 )
Research paper	Mueller et al., 2005, Genetics 171(3): 1137--1152 Genetic modifier screens on hairless gain-of function phenotypes reveal genes involved in cell differentiation, cell growth and apoptosis in Drosophila melanogaster. [FBrf0190751] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Pena-Rangel et al., 2002, Genetics 160(3): 1035--1050 A misexpression study examining dorsal thorax formation in Drosophila melanogaster. [FBrf0147055] Tseng and Hariharan, 2002, Genetics 162(1): 229--243 An overexpression screen in Drosophila for genes that restrict growth or cell-cycle progression in the developing eye. [FBrf0152035] Benos et al., 2001, Genome Res. 11(5): 710--730 From First Base: the sequence of the tip of the X chromosome of Drosophila melanogaster, a comparison of two sequencing strategies. [FBrf0135823] Abdelilah-Seyfried et al., 2000, Genetics 155(2): 733--752 A gain-of-function screen for genes that affect the development of the Drosophila adult external sensory organ. [FBrf0128367] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}Hr4EP1232 Insertion

Dmel\P{EP}Hr4^EP1232 Insertion