A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}EP1321EP1321 Insertion

General Information
Symbol Dmel\P{EP}EP1321EP1321 Species D. melanogaster
Name FlyBase ID FBti0011863
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) CG2145, EP1321 Viability / fertility
Causes allele(s) EP1321EP1321 Stock availability 1 publicly available
LINE ID EP(X)1321
Genomic Location
Chromosomal location X ( 9F12 ) Sequence location X:10,824,836..10,824,836 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0063906 FBti0014734 FBti0014737 FBti0014733 FBti0014735 FBti0035772 FBti0131890 FBti0033513 FBti0070507 FBti0025143 FBti0011863 FBti0055988 FBti0052988 FBti0035715 FBti0143644
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
X:10,824,836..10,824,836 [-]
(BDGP Project Members, 2000-)
Orientation
Cytological location
(computed by FlyBase)
9F12 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
10A1-10A1 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
10A1-10A2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ073188
(FlyBase, 1992-,  
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
CG2145
(BDGP Project Members, 2000-)
EP1321
(Kraut et al., 2001, Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
EP1321EP1321
(Kraut et al., 2001, Gene Disruption Project members, 2001-)
Lethality
References
Sterility
References
hide Phenotype Manifest In
anterior fascicle & synapse
(Kraut et al., 2001)
anterior fascicle & synapse | supernumerary
(Kraut et al., 2001)
intersegmental nerve
(Kraut et al., 2001)
hide Detailed Description
Statement
Reference
Mutant larvae expressing EP1321EP1321 under the control of Scer\GAL4elav-C155 have excessive or ectopic synapses. Mutant larvae expressing EP1321EP1321 under the control of Scer\GAL4elav-C155 have reduced or abnormal synapses.
(Kraut et al., 2001)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(X)1321
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11290
w1118 P{EP}EP1321EP1321
hide Linkouts
hide Comments
Location X:10776021-10776022 confirmed by FlyBase alignment of dbGSS accession AQ073188 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EP(1)1321
(Semeshin et al., 2008)
EP(x) 1321
(Kraut et al., 2001)
EP(X)1321
(FlyBase, 1992-)
P{EP}EP1321
(FlyBase, 1992-)
P{EP}EP1321EP1321
(FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 11 )
Research paper
Semeshin et al., 2008, Genetica 132(3): 267--279
Interbands behave as decompacted autonomous units in Drosophila melanogaster polytene chromosomes. [FBrf0203085]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Marcus, 2003, Genetics 163(2): 591--597
Female site-specific transposase-induced recombination: a high-efficiency method for fine mapping mutations on the X chromosome in Drosophila. [FBrf0157240]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Supplementary material
Kraut et al., 2001, Curr. Biol. 11(6):
A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. [FBrf0135968]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]