A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{EP}moodyEP1529 Insertion

General Information
Symbol Dmel\P{EP}moodyEP1529 Species D. melanogaster
Name FlyBase ID FBti0011935
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) CG4313, moody Viability / fertility
Causes allele(s) moodyEP1529 Stock availability 1 publicly available
LINE ID EP(X)1529
Genomic Location
Chromosomal location X ( 2C8 ) Sequence location X:1,943,646..1,943,646 [-]
Map ( GBrowse ) detailed view FBti0131883 FBti0037354 FBti0039836 FBti0071296 FBti0007384 FBti0037230 FBti0029710 FBti0023555 FBti0029347 FBti0048814 FBti0029352 FBti0048211 FBti0052570 FBti0070115 FBti0011935 FBti0056333 FBti0007542 FBti0054603 FBti0108518 FBti0021919 FBti0068632
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
X:1,943,646..1,943,646 [-]
(BDGP Project Members, 2000-)
Orientation
Cytological location
(computed by FlyBase)
2C8 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
2C4-2C4 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
2C7-2C8 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ073244
(Benos et al., 2001, FlyBase, 1992-,  
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
CG4313
(Benos et al., 2001)
moody
(BDGP Project Members, 2000-, Benos et al., 2001)
hide Alleles and Phenotypes
Causes alleles
moodyEP1529
(Kunwar et al., 2003, Bainton et al., 2005)
Lethality
References
viable
(Bainton et al., 2005)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
moodyEP1529 flies are fully viable and show normal levels of climbing and locomotion. However, these flies show increased sensitivity to cocaine and nicotine, as measured by their behavorial responses. In contrast, these flies are resistant to the intoxicating effects of ethanol as they have an increased mean elution time from the inebriometer, although their ethanol absorbance is normal. The blood-brain barrier is not affected in moodyEP1529 flies.
(Bainton et al., 2005)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(X)1529
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
17007
w1118 P{EP}moodyEP1529
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hide Comments
Location X:1905769-1905770 confirmed by FlyBase alignment of dbGSS accession AQ073244 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
Maps to clone: 22E5
(Benos et al., 2001)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EP1529
(Bainton et al., 2005)
EP(1)1529
(Benos et al., 2001)
EP(X)1529
(FlyBase, 1992-)
P{EP}CG4322EP1529
(FlyBase, 2005, FlyBase, 1992-)
P{EP}EP1529
(FlyBase, 1992-)
P{EP}moodyEP1529
(FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 11 )
Research paper
Bainton et al., 2005, Cell 123(1): 145--156
moody encodes two GPCRs that regulate cocaine behaviors and blood-brain barrier permeability in Drosophila. [FBrf0189897]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Kunwar et al., 2003, PLoS Biol. 1(3): 372--384
Tre1, a G protein-coupled receptor, directs transepithelial migration of Drosophila germ cells. [FBrf0167988]
Benos et al., 2001, Genome Res. 11(5): 710--730
From First Base: the sequence of the tip of the X chromosome of Drosophila melanogaster, a comparison of two sequencing strategies. [FBrf0135823]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Rorth, 1996, Proc. Natl. Acad. Sci. USA 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0125078]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]