Dmel\P{SUPor-P}polo^16-1 Insertion

General Information
Symbol	Dmel\P{SUPor-P}polo^16-1	Species	D. melanogaster
Name		FlyBase ID	FBti0012155
Feature type	transposable_element_insertion_site
Description
Inserted element	P{SUPor-P}	Expression data
Affected gene(s)	polo	Viability / fertility
Causes allele(s)	polo^16-1	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	3L ( 77B2-77B3 )	Sequence location
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	77B2-77B3 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{SUPor-P}
Location-dependent role
Size	11.467Kb
Associated alleles	gypsy\su(Hw)BR⁴³⁰ w^BR.E.BR y^+mDint2
Molecular map
Affected Gene(s)
Insertion may affect gene	polo (Roseman et al., 1995)
Alleles and Phenotypes
Causes alleles	polo^16-1 (Roseman et al., 1995)

Lethality References lethal \| recessive (Roseman et al., 1995)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference Heterozygosity for polo[16-1] does not result in increased levels of achiasmate X or 4th chromosome non-disjunction in In(1)FM7/X females (the In(1)FM7 chromosome is present to fully suppress X chromosomal exchange). (Xiang et al., 2007) polo[KG03033]/+ suppresses the elevated levels of achiasmate X and 4th chromosome nondisjunction seen in In(1)FM7/X mtrm[126]/+ females. The precocious nuclear envelope breakdown (NEB) seen in stage 12 oocytes of mtrm[126]/+ females is strongly suppressed by polo[16-1]/+. The defects in karyosome morphology seen in mtrm[126]/+ oocytes observed within 20 minutes of NEB are also largely suppressed by polo[16-1]/+. polo[16-1]/+ largely suppresses the karyosome maintenance defects seen in mtrm[126]/+ oocytes. polo[16-1]/+ fully suppresses the defect in X and 4th chromosome centromere co-orientation that is seen in mtrm[126]/+ females. (Xiang et al., 2007) sub[1]/sub[131]; polo[16-1]/+ rare survivors are sickly and small with abdominal patterning defects. Late larval developmental arrest is observed. Severe defects in spindle organization are observed, and spindles are unusually short. Larvae show a higher percentage of cells in mitosis compared to sub[1]/sub[131] or polo[16-1]/+, and nearly four times that of wild-type. (Cesario et al., 2006) polo¹/polo^16-1 females do not show gross defects in meiotic spindle formation. (Jang et al., 2005)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	4565 P{SUPor-P}polo^16-1/MKRS
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{SUPor-P}l(3)77Aa^16-1 (Roseman et al., 1995, FlyBase, 1992-) P{SUPor-P}polo^16-1 (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 5 )
Research paper	Xiang et al., 2007, PLoS Biol. 5(12): e323 The inhibition of polo kinase by matrimony maintains G2 arrest in the meiotic cell cycle. [FBrf0202869] Cesario et al., 2006, J. Cell Sci. 119(Pt 22): 4770--4780 Kinesin 6 family member Subito participates in mitotic spindle assembly and interacts with mitotic regulators. [FBrf0192855] Jang et al., 2005, Molec. Biol. Cell 16(10): 4684--4694 The kinesinlike protein Subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytes. [FBrf0188037] Roseman et al., 1995, Genetics 141(3): 1061--1074 A P element containing suppressor of Hairy-wing binding regions has novel properties for mutagenesis in Drosophila melanogaster. [FBrf0084323]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]

Dmel\P{SUPor-P}polo16-1 Insertion

Dmel\P{SUPor-P}polo^16-1 Insertion