Dmel\P{lacW}mth¹ Insertion

General Information
Symbol	Dmel\P{lacW}mth¹	Species	D. melanogaster
Name		FlyBase ID	FBti0012557
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	mth	Viability / fertility
Causes allele(s)	mth¹	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	3L ( 61C1 )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	61C1 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	mth (Lin et al., 1998, Ja et al., 2009)
Alleles and Phenotypes
Causes alleles	mth¹ (Lin et al., 1998, Song et al., 2002, Ja et al., 2009)

Lethality References viable (Lin et al., 1998, Ja et al., 2009)
Sterility References fertile (Lin et al., 1998)
Phenotype Manifest In
	male germline stem cell (Wallenfang et al., 2006)
Detailed Description
Statement Reference mth[1] mutant larvae exposed to 2.0-5.0 μg/mL of endosulfan showed significantly higher survival rates compared to controls. (Sharma et al., 2011) Mutant neuromuscular junctions show reliable homeostatic compensation (increase in quantal content) after treatment with philanthotoxin-433 for 10 minutes. (Dickman and Davis, 2009) Animals bearing mth[Δ] in trans to mth[1] are viable and show no obvious morphological or behavioural defects. (Ja et al., 2009) Animals bearing mth[Δ] in trans to mth[1] are viable and show no obvious morphological or behavioural defects. Virgin female mth[Δ]/mth[1] mutants exhibit normal ovulation and mating frequencies after Acp70A injection. (Ja et al., 2009) Germline stem cells (GSCs) from newly eclosed mutant males show an S-phase index similar to wild-type. No decrease in S-phase index is seen in GSCs from mutant males that have been aged for 35 days, in contrast to wild-type aged controls. (Wallenfang et al., 2006) Mutant flies show increased survival under hyperoxia (100% oxygen) compared to controls. (Walker and Benzer, 2004) Mutants exhibit no gross morphological defects in the embryonic nervous system. In mth¹ homozygotes, the amplitudes of evoked excitatory junctional potentials (EJPs) are reduced to about 63% of those seen in controls. In mth¹/mth^Δ6 they reduced to about 57%. Electronic stimulation in the presence of 1uM Tetrodotoxin (TTX, a Na⁺ channel inhibitor) at a intensity of 2.5 fold over threshold abolishes nerve-evoked EJPs as also seen in wild-type. Stimulation with intensities 40-fold over threshold elicits saturated EJPs also as normal, however the electrotonic EJPs recorded from mutants are reduced to about half of control levels. The mean amplitude, the cumulative amplitude distribution, and the frequency of unitary mEJPs in mutant larvae show no significant differences from controls. The quantal content of evoked release in mutant neuromuscular junctions is equally reduced for all ^Ca<up>2+</up>_es. At 1mM^Ca<up>2+</up>_e, the quantal content is reduced to about half that of controls, and to about 40% in mth¹/mth^Δ6. Evoked excitatory junctional currents (EJCs) are reduced to about half of control amplitudes in mutants. Saturated extracellular EJPs are also significantly reduced to almost quarter of those in controls. Depolarisation-dependent Ca²⁺ entry and/or Ca²⁺ extrusion are normal in mutants. Calcimycin-induced release on mutants is significantly reduced to about half of control levels. The effect of latroinsectoxins on the frequency of mEJPs in mutants is indistinguishable from controls. The size of synaptic areas and the density of their associated vesicle populations are abnormal in mutant boutons. The mean synaptic area is reduced ro about 55% of controls. In addition the density of synaptic vesicles within 0-500 nm from the plasma membrane of a synaptic site is reduced to about a 2/3 of controls. The density of vesicles clustered around a synaptic site is also reduced. The density of vesicles within a distance of 0-50 nm and 50-300 nm from a synaptic area is reduced to about 3/4 and 2/3 of controls respectively. The density of vesicles located further away is not significantly reduced. During high frequency stimulation at 10Hz control EJPs depress within 3 s of stimulation to about 75% of their initial amplitude and are thereafter sustained, while in mutants, EJPs show neither an initial depression nor fatigue during the sustained period. Increasing the stimulation frequency to 30 Hz has no further effects on mutant amplitudes, while controls are depressed further. Phorbol 12-myristate 13-acetate (PMA) induced facilitation is absent in homozygous mutants at 0.5 mM ^Ca<up>2+</up>_e (as compared to wild-type where an increase in EJP amplitudes to ~238% of controls is seen). However at a ^Ca<up>2+</up>_e of 0.7 mM, PMA slightly facilitates evoked release in mutants. (Song et al., 2002) Homozygous flies live on average 35% longer than control flies at both 25^oC and 29^oC. Homozygous adults are more resistant to dietary paraquat, show a greater than 50% increase in average survival time in a starvation test and survive longer at 36^oC than control flies. Homozygous males and females weigh 20 to 30% more than control flies. (Lin et al., 1998)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	27896 w^*; P{lacW}mth¹
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}mth¹ (Lin et al., 1998, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 9 )
Research paper	Sharma et al., 2011, Chemosphere 82(3): 370--376 Transcriptome analysis provides insights for understanding the adverse effects of endosulfan in Drosophila melanogaster. [FBrf0212610] Dickman and Davis, 2009, Science 326(5956): 1127--1130 The schizophrenia susceptibility gene dysbindin controls synaptic homeostasis. [FBrf0209454] Ja et al., 2009, Protein Sci. 18(11): 2203--2208 The Drosophila G protein-coupled receptor, Methuselah, exhibits a promiscuous response to peptides. [FBrf0209193] Wallenfang et al., 2006, Aging Cell 5(4): 297--304 Dynamics of the male germline stem cell population during aging of Drosophila melanogaster. [FBrf0193116] Walker and Benzer, 2004, Proc. Natl. Acad. Sci. U.S.A. 101(28): 10290--10295 Mitochondrial 'swirls' induced by oxygen stress and in the Drosophila mutant hyperswirl. [FBrf0179503] Song et al., 2002, Neuron 36(1): 105--119 Presynaptic regulation of neurotransmission in Drosophila by the G protein-coupled receptor Methuselah. [FBrf0152269] Lin et al., 1998, Science 282(5390): 943--946 Extended life-span and stress resistance in the Drosophila mutant methuselah. [FBrf0105270] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacW}mth1 Insertion

Dmel\P{lacW}mth¹ Insertion