A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{PZ}l(3)04704a04704a Insertion

General Information
Symbol Dmel\P{PZ}l(3)04704a04704a Species D. melanogaster
Name FlyBase ID FBti0012631
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data
Affected gene(s) l(3)04704a Viability / fertility
Causes allele(s) l(3)04704a04704a Stock availability none publicly available
LINE ID
Genomic Location
Chromosomal location Sequence location
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
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Description
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FB2013_03
FB2013_02
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Chromosome (arm)
Sequence Location
Orientation
Cytological location
(computed by FlyBase)
Cytological location
(reported)
Comments concerning
location
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Flanking sequence
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
Size 14.545Kb
Associated alleles
Molecular map
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Insertion may
affect gene
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Causes alleles
Lethality
References
lethal
 
Sterility
References
hide Phenotype Manifest In
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Statement
Reference
The lethality caused by sevS11.T:Hsap\MYC in combination with Ras85DV12.sev is suppressed by the l(3)04704 line. The l(3)04704 line has two P{PZ} insertions: P{PZ}l(3)04704a04704a at 90D1--90D2 and P{PZ}l(3)04704b04704b at 97E5--97E6. It is not known which P{PZ} insertion in the l(3)04704 line is responsible for the suppression of the lethality caused by sevS11.T:Hsap\MYC in combination with Ras85DV12.sev.
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Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
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FlyView (LinkOut)
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Line ID
Origin as a multiple insertion line
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Aberration
Balancer
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Reported As
Symbol Synonym
P{PZ}l(3)04704a04704a
Secondary FlyBase IDs
hide References ( 6 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Maixner et al., 1998, Dev. Genet. 23(4): 347--361
A screen for mutations that prevent lethality caused by expression of activated Sevenless and Ras1 in the Drosophila embryo. [FBrf0105879]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]