A Database of Drosophila Genes & Genomes

FB2008_06, released July 3, 2008
 

Dmel\P{lacW}ActnG0077 Insertion

General Information
Symbol Dmel\P{lacW}ActnG0077 Species D. melanogaster
Name FlyBase ID FBti0012876
Feature type transposable_element_insertion_site Created / Updated 1999-06-27/1999-06-27
Description
Inserted element P{lacW} Localized function
Affected gene(s) Actn Expression data
Causes allele(s) ActnG0077 Viability / fertility
LINE ID P{lacW}l(1)G0077 Stock availability
Genomic Location
Chromosomal location X ( 2C5 ) Sequence location X:1,926,550..1,927,277 [-]
Map ( GBrowse ) detailed view FBti0018242 FBti0056593 FBti0033667 FBti0075853 FBti0012876 FBti0099714 FBti0057940 FBti0037354 FBti0039836 FBti0071296 FBti0007384 FBti0037230 FBti0029347 FBti0023555 FBti0029710 FBti0029352 FBti0048814 FBti0048211
hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
X:1,926,550..1,927,277 [-]
(FBrf0199566)
Orientation
Cytological location
(computed by FlyBase)
2C5 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
2C1--2C2 (in situ hybridization reported)
(Peter et al., 2002)
Chromosome in situ
hide Sequence Data
Flanking sequence
AJ300034
(Benos et al., 2001, FlyBase, 1992-, Peter et al., 2002, FBrf0199566)
hide Inserted Element
Construct P{lacW}
Location-dependent
role 		 
Size 10.691Kb
Associated alleles
w+mC
Ecol\lacZP\T.W
Molecular map
Transposon class
Element type
hide Affected Gene(s)
Insertion within gene
Insertion may
affect gene
Actn
(Schaefer et al., 1999.3.29)
Orientation
(relative to gene)
Gene
Orientation
References
 
 
 
hide Alleles and Phenotypes
Causes alleles
ActnG0077
(Peter et al., 2002, Schaefer et al., 1999.6.15, Schaefer et al., 1999.3.29, Benos et al., 2001)
Lethality
References
lethal | larval stage
(Peter et al., 2002)
lethal | prepupal stage
(Peter et al., 2002)
lethal | pupal stage
(Peter et al., 2002)
lethal | recessive
(Schaefer et al., 1999.3.29)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
ActnG0077 vriunspecified double heterozygotes have progressive locomotory defects affecting the rear legs. vri5R7.2 ActnG0077 double heterozygotes have missing hairs at the anterior wing margin and on the wing surface.
(Szuplewski et al., 2003)
Lethality occurs from the third larval instar through to the pupal stage.
(Peter et al., 2002)
hide Expression Data
Reporter Expression
Stage
Tissue/Position
Reference
Additional Information
Statement
Reference
Assay mode
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
P{lacW}l(1)G0077
(FBrf0199566)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11802
P{lacW}ActnG0077 w67c23/FM7c
hide Linkouts
hide Comments
Maps to clone: 22E5
(Benos et al., 2001)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}ActnG0077
(Benos et al., 2001, Peter et al., 2002, FlyBase, 1992-, Schaefer et al., 1999.3.29)
Secondary FlyBase IDs
    hide References ( 10 )
    Research paper
    Beinert et al., 2004, Chromosoma 113(6): 271--275
    Systematic gene targeting on the X chromosome of Drosophila melanogaster. [FBrf0183806]
    Szuplewski et al., 2003, Development 130(16): 3651--3662
    The Drosophila bZIP transcription factor Vrille is involved in hair and cell growth. [FBrf0160992]
    Benos et al., 2001, Genome Res. 11(5): 710--730
    From First Base: the sequence of the tip of the X chromosome of Drosophila melanogaster, a comparison of two sequencing strategies. [FBrf0135823]
    Roulier et al., 1992, J. Cell Biol. 116(9-10): 911--922
    Perturbations of Drosophila -actinin cause muscle paralysis, weakness, and atrophy but do not confer obvious nonmuscle phenotypes. [FBrf0056452]
    Sparrow et al., 1991, J. molec. Biol. 222: 963--982
    Functional and ultrastructural effects of a missense mutation in the indirect flight muscle-specific actin gene of Drosophila melanogaster. [FBrf0054553]
    Supplementary material
    Peter et al., 2002, EMBO Rpts 3(1):
    Supplementary material. PX-lines.txt. [FBrf0173176]
    Personal communication to FlyBase
    Schaefer et al., 1999.3.29, P{lacW} insertions.
    P{lacW} insertions. [FBrf0107552]
    Schaefer et al., 1999.6.15, Goettingen lethals - set 2.
    Goettingen lethals - set 2. [FBrf0108529]
    FlyBase analysis
    FlyBase, 1992-, FlyBase curation
    FlyBase curation. [FBrf0105495]
    Assessment of transgenic construct insertion sites. [FBrf0199566]