Dmel\P{lacW}trol^G0271 Insertion

General Information
Symbol	Dmel\P{lacW}trol^G0271	Species	D. melanogaster
Name		FlyBase ID	FBti0013150
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	trol	Viability / fertility
Causes allele(s)	trol^G0271	Stock availability	2 publicly available
LINE ID	P{lacW}l(1)G0271
Genomic Location
Chromosomal location	X ( 3A4 )	Sequence location	X:2,411,669..2,412,176 [-]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	X:2,411,669..2,412,176 [-] (FlyBase, 2007.6)
Orientation
Cytological location (computed by FlyBase)	3A4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	3A3--3A4 (in situ hybridization reported) (Peter et al., 2002)
Comments concerning location
Sequence Data
Flanking sequence	AJ300055 (Benos et al., 2001, FlyBase, 1992-, Peter et al., 2002, FlyBase, 2007.6)
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	trol (Schaefer et al., 1999.6.15, Voigt et al., 2002, Peter et al., 2002)
Alleles and Phenotypes
Causes alleles	trol^G0271 (Schaefer et al., 1999.6.15, Peter et al., 2002, Voigt et al., 2002)

Lethality References lethal \| larval stage (Peter et al., 2002) lethal \| prepupal stage (Peter et al., 2002) lethal \| pupal stage (Peter et al., 2002) lethal \| recessive (Schaefer et al., 1999.6.15) lethal \| recessive \| third instar larval stage (Voigt et al., 2002)
Sterility References
Phenotype Manifest In
	embryonic/larval brain (Voigt et al., 2002) embryonic/larval optic lobe (Voigt et al., 2002) haltere disc (Voigt et al., 2002) wing disc (Voigt et al., 2002)
Detailed Description
Statement Reference Lethality occurs from the third larval instar through to the pupal stage. (Peter et al., 2002) Hemizygous larvae develop smaller optic lobes than controls, while other parts of the brain show overgrowth defects. The thoracic ganglion region is disrupted. The wing discs of hemizygous larvae are convoluted and reduced in size compared to controls. The wing pouch is rotated 90[o] relative to the dorsoventral axis and the dorsal body wall region is heavily reduced in size. The haltere disc is heavily convoluted in the mutant larvae. Hemizygous larvae take at least 1 day longer to reach third larval instar compared to controls, and eventually die as third instar larvae. (Voigt et al., 2002)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	P{lacW}l(1)G0271 (FlyBase, 2007.6)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	11848 P{lacW}trol^G0271 w^67c23/FM7c
Kyoto	111803 P{lacW}trol^G0271 w^* P{neoFRT}19A/FM7c; P{ey-FLP.N}5
Linkouts
Comments
	Maps to clone: BACR25B3 (Benos et al., 2001)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}l(1)G0271^G0271 (Schaefer et al., 1999.6.15, Peter et al., 2002, FlyBase, 1992-) P{lacW}trol^G0271
Secondary FlyBase IDs

References ( 7 )
Research paper	Voigt et al., 2002, Dev. Dynamics 224(4): 403--412 Perlecan participates in proliferation activation of quiescent Drosophila neuroblasts. [FBrf0151905] Benos et al., 2001, Genome Res. 11(5): 710--730 From First Base: the sequence of the tip of the X chromosome of Drosophila melanogaster, a comparison of two sequencing strategies. [FBrf0135823] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Peter et al., 2002, EMBO Rpts 3(1): Supplementary material. PX-lines.txt. [FBrf0173176]
Personal communication to FlyBase	Schaefer et al., 1999.6.15, Goettingen lethals - set 2. Goettingen lethals - set 2. [FBrf0108529]
FlyBase analysis	FlyBase, 2007.6, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0199566] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]

Dmel\P{lacW}trolG0271 Insertion

Dmel\P{lacW}trol^G0271 Insertion