FB2025_01 , released February 20, 2025
Insertion: Dmel\P{GawB}NP5130
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General Information
Symbol
Dmel\P{GawB}NP5130
Species
D. melanogaster
Name
FlyBase ID
FBti0013268
Feature type
transposable_element_insertion_site
Also Known As
esg-GAL4, esgGal4, escargot-gal4, NP5130, esgGal4NP5130, Esg-GAL4NP5130, esg::Gal4
Description
Inserted Element
P{GawB}
LINE ID

NP5130-3-1

Affected gene(s)
Scer\GAL4
Viability / fertility
fertile, viable
Associated allele(s)
Scer\GAL4NP5130
Stock availability
11 publicly available
Genomic Location
Chromosomal Location
2L ( 35D2 )
Sequence Location
2L:15,333,772..15,333,772 [-]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{GawB}.NP
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:15,333,772..15,333,772 [-]
(FlyBase, 2007.6)
Orientation
Cytological location (computed by FlyBase)
35D2 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Inserted Element
Construct
P{GawB}
(FlyBase, 2007.6)
Size (Kb)

11.279

Component Allele(s)
Scer\GAL4wB
w+mW.hs
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
intestinal stem cell
(Ma et al., 2013, Choi et al., 2008)
enteroblast
(Ma et al., 2013, Choi et al., 2008)
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 14
embryonic/larval heart anchoring cell
(Zmojdzian et al., 2008)
larval stage
larval imaginal tissue
(Martin and Morata, 2006)
larval stage -- pupal stage
histoblast
(Foronda et al., 2012)
histoblast nest
(Foronda et al., 2012)
second instar larval stage
leg disc | restricted
(Goto and Hayashi, 1999)
third instar larval stage
leg disc | proximal
(Goto and Hayashi, 1999)
embryonic/larval spiracular tracheal branch | distal | restricted
(Pitsouli and Perrimon, 2010)
pupal stage | male
anterior dorsal histoblast nest abdominal 6
(Foronda et al., 2012)
anterior dorsal histoblast nest abdominal 7
(Foronda et al., 2012)
posterior dorsal histoblast nest abdominal 6
(Foronda et al., 2012)
posterior dorsal histoblast nest abdominal 7
(Foronda et al., 2012)
adult stage
intestinal stem cell
(Guo et al., 2013, Buchon et al., 2010)
enteroblast
(Guo et al., 2013)
AC thermosensory neuron
(Jin et al., 2021)
adult stage | female
intestinal stem cell
(Micchelli and Perrimon, 2006)
enteroblast
(Micchelli and Perrimon, 2006)
Additional Information
Statement
Reference

Drives expression in histoblasts and histoblast nests, in enteroblasts, and in intestinal stem cells.

(FlyBase Curators, 2017)

ScerGAL4esg-NP5130 drives expression in abdominal histoblast nests and histoblasts, but not in cuticular larval epidermal cells.

(Foronda et al., 2012)

ScerGAL4esg-NP5130 expression is observed in 36% of small cells that occupy a basal position within the adult midgut epithelium.

(Micchelli and Perrimon, 2006)

About half of esg-positive (ScerGAL4esg-NP5130 expression) cells lack N signalling (do not express EcollacZDdc.be2.E(spl)m8-HLH); these exhibit characteristics of intestinal stem cells and enteroblasts.

(Micchelli and Perrimon, 2006)

Expression in second instar leg discs is excluded from the distal-most regions. Third instar expression is observed in the proximal region of the leg discs.

(Goto and Hayashi, 1999)
 
Marker for
embryonic/larval heart anchoring cell
(Zmojdzian et al., 2008)
enteroblast
(Lin et al., 2013, Ma et al., 2013, Choi et al., 2008)
histoblast
(Foronda et al., 2012)
histoblast nest
(Foronda et al., 2012)
intestinal stem cell
(Lin et al., 2013, Ma et al., 2013, Choi et al., 2008)
Reflects expression of
esg
(Guo et al., 2013, Lin et al., 2013, Ma et al., 2013, Foronda et al., 2012, Buchon et al., 2010, Zmojdzian et al., 2008, Martin and Morata, 2006, Micchelli and Perrimon, 2006, Kondoh et al., 2003, Goto and Hayashi, 1999)
Reporter construct used in assay
Avic\GFPEGFP.nls.UAS.Tag:MYC
(Foronda et al., 2012)
Mmus\Cd8aUAS.cLa.EGFP
(Buchon et al., 2010, Micchelli and Perrimon, 2006)
tkvGD2549
(Guo et al., 2013)
Avic\GFPUAS.cUa
(Ma et al., 2013, Choi et al., 2008)
Data on Genetic Line
Line ID

NP5130-3-1

(FlyBase, 2007.6)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (11)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Location 2L:15329700-15329701 refined by FlyBase alignment of dbGSS accession AG218990 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)
Synonyms and Secondary IDs (23)
Reported As
Symbol Synonym
Escargot-Gal4
(Osman et al., 2012, Narbonne-Reveau et al., 2011)
Esg-GAL4
(Vonolfen et al., 2024, Hatini et al., 2013, Cronin et al., 2009, Zmojdzian et al., 2008)
Esg-GAL4NP5130
(Hatini et al., 2013)
Esg-Gal4
(Joshi et al., 2023, Saavedra et al., 2023, Xu et al., 2023, Kaur et al., 2022, Kim et al., 2021, Petrovsky and Großhans, 2018, Yu et al., 2015, Huang et al., 2014, Zeng et al., 2010, Ninov et al., 2007)
NP5130
(Wu et al., 2023, Liu et al., 2022, Zhao et al., 2021, Harsh et al., 2018, Ninov et al., 2007)
NP5130-GAL4
(Jin et al., 2021)
P{GAL4}NP5130
(FlyBase, 1992-)
P{GawB}NP5130
(Mukherjee et al., 2021, FlyBase, 1992-)
P{GawB}NP5130-3-1
(FlyBase, 2005, FlyBase, 1992-)
escargo Gal4
(Venkei et al., 2006)
escargot-GAL4
(Berns et al., 2014, Cronin et al., 2009)
escargot-Gal4
(Tang et al., 2013)
escargot-gal4
(Cordero et al., 2014)
esg-GAL4
(Xu et al., 2023, Chen et al., 2022, Buddika et al., 2021, Ding et al., 2021, Resnik-Docampo et al., 2021, Iijima et al., 2020, Upadhyay et al., 2020, Nakano et al., 2019, Kwon et al., 2015, Park et al., 2015, Banerjee and Chinthapalli, 2014, Jin et al., 2013, Lee et al., 2013, Bangi et al., 2012, Glittenberg et al., 2011, Ngo et al., 2010, Park et al., 2010, Choi et al., 2008, Lee and Kolodziej, 2002, Goto and Hayashi, 1999)
esg-GAL4NP5130
(Jneid et al., 2023)
esg-Gal4
(Gao et al., 2024, Nagai et al., 2024, Zhang et al., 2024, Jneid et al., 2023, Karuparti et al., 2023, Loudhaief et al., 2023, Nagai et al., 2023, Socha et al., 2023, Buddika et al., 2022, Chen et al., 2022, Liu et al., 2022, Liu et al., 2022, Mukherjee et al., 2022, Neophytou and Pitsouli, 2022, Wang and Spradling, 2022, Koreman et al., 2021, Mok and Choi, 2021, Nagai et al., 2021, Nászai et al., 2021, Nirala et al., 2021, Parkhitko et al., 2021, Tamamouna et al., 2021, Tang et al., 2021, Wu et al., 2021, Arnaoutov et al., 2020, Bajpai et al., 2020, Jin et al., 2020, Weaver et al., 2020, Gultekin and Steller, 2019, Wang et al., 2019, Harsh et al., 2018, Iatsenko et al., 2018, Obata et al., 2018, Xu et al., 2018, Yin and Xi, 2018, Zhai et al., 2018, Ngo et al., 2017, Tian et al., 2017, Zhai et al., 2017, Nagy et al., 2016, Singh et al., 2016, Wang et al., 2016, Beehler-Evans and Micchelli, 2015, Jin et al., 2015, Liu et al., 2015, Yu et al., 2015, Markstein et al., 2014, Montagne and Gonzalez-Gaitan, 2014, Song et al., 2014, Buchon et al., 2013, Guo et al., 2013, Karpac et al., 2013, Ma et al., 2013, Pitsouli and Perrimon, 2013, Tang et al., 2013, Wang et al., 2013, Lee et al., 2011, Biteau et al., 2010, Buchon et al., 2010, Ninov et al., 2010, Pitsouli and Perrimon, 2010, Ren et al., 2010, Buchon et al., 2009, Chatterjee and Ip, 2009, McKay et al., 2009, Villányi et al., 2008, Martin and Morata, 2006, Micchelli and Perrimon, 2006, Kondoh et al., 2003, Goto and Hayashi, 1999)
esg-Gal4NP5130
(Biteau and Jasper, 2014)
esg-gal4
(Bond and Foley, 2012)
esg::Gal4
(Wang et al., 2015, Wang et al., 2014)
esgNP5130-Gal4
(Requena et al., 2017)
esgP{GawB}NP5130-GAL4
(Buddika et al., 2021)
esgGal4
(Zhao et al., 2022, Lu et al., 2021, Zhao et al., 2021, Xu et al., 2020, Xu et al., 2019, Lu and Li, 2015, Meng and Biteau, 2015, Nie et al., 2015, Biteau and Jasper, 2014, Huang et al., 2014, Yu et al., 2014, Karpac et al., 2013, Lin et al., 2013, Ma et al., 2013, Quan et al., 2013, Biteau et al., 2010, Wang et al., 2010, Lee et al., 2009)
esgGal4NP5130
(Meng et al., 2020, McClelland et al., 2017, Meng and Biteau, 2015)
Name Synonyms
Secondary FlyBase IDs
    References (138)