Dmel\P{hs-Fas3.K}HF-ET Insertion

General Information
Symbol	Dmel\P{hs-Fas3.K}HF-ET	Species	D. melanogaster
Name		FlyBase ID	FBti0013401
Feature type	transposable_element_insertion_site
Description
Inserted element	P{hs-Fas3.K}	Expression data
Affected gene(s)	Fas3	Viability / fertility
Causes allele(s)	Fas3^HF-ET	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	2L ( 36F2-36F4 )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	36F2-36F4 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{hs-Fas3.K}
Location-dependent role
Size
Associated alleles	Fas3^hs.PK
Molecular map
Affected Gene(s)
Insertion may affect gene	Fas3 (Kose et al., 1997)
Alleles and Phenotypes
Causes alleles	Fas3^HF-ET (Kose et al., 1997)

Lethality References
Sterility References
Phenotype Manifest In
	abdominal segmental nerve (Kose et al., 1997) aCC neuron (Kose et al., 1997)
Detailed Description
Statement Reference The aCC growth cone extends normally through the ISN pathway and reliably selects its normal target muscle in Fas3^HF-ET flies, despite misexpressing Fas3 during axon pathfinding and target selection. SNa innervation is normal in Fas3^HF-ET flies, despite misexpression of Fas3 in the SNa motorneurons during axon pathfinding. Muscle 1 lacks innervation in 4% of cases in embryos carrying both Fas3^Mhc.PC and Fas3^HF-ET. The aCC growth cone stops short of muscle 1 and instead bends its head slightly posteriorly along muscle 2 in 25% of cases in these embryos. Muscle 1 lacks innervation in 12% of cases in embryos carrying both Fas3^Mhc.PC and Fas3^HF-ET and expressing Fas3^hs.PK (which is present in the P{hs-Fas3.K}HF-ET insertion) due to heat shock. The aCC growth cone mistargets muscle 2 in 30% of cases in these embryos. Abnormal axon collaterals stem out of the SNa nerve in 17% of cases in 18 hour embryos carrying both Fas3^Mhc.PC and Fas3^HF-ET. These collaterals are thin, probably consisting of only one or two axons, and misinnervate muscles 12, 13, 26 or 27. (Kose et al., 1997)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{hs-Fas3.K}HF-ET (Kose et al., 1997, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 2 )
Research paper	Kose et al., 1997, Development 124(20): 4143--4152 Homophilic synaptic target recognition mediated by immunoglobulin-like cell adhesion molecule Fasciclin III. [FBrf0099327]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]