Dmel\P{lacW}trc^S066917a Insertion

General Information
Symbol	Dmel\P{lacW}trc^S066917a	Species	D. melanogaster
Name		FlyBase ID	FBti0013685
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	trc	Viability / fertility
Causes allele(s)	trc^S066917a	Stock availability	1 publicly available
LINE ID	S066917
Genomic Location
Chromosomal location	3L ( 76D1 )	Sequence location	3L:19,826,530..19,826,530 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:19,826,530..19,826,530 [-] (Levis and Hoskins R., 2012.1.10)
Orientation	- (Levis and Hoskins R., 2012.1.10) + (Geng et al., 2000)
Cytological location (computed by FlyBase)	76D1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	76C4--76C6 (in situ hybridization reported) (Deak et al., 1997) 76D1_r5 (reported as inferred from sequence location) (Levis and Hoskins R., 2012.1.10)
Comments concerning location	Genomic location previously reported as 3L:19826239..19,827,520 [+] . (Geng et al., 2000) The insertion site was mapped to a BamHI-HindIII restriction fragment. The orientation of the insertion was not reported. (Geng et al., 2000)
Sequence Data
Flanking sequence	JM973014 (Levis and Hoskins R., 2012.1.10)
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	trc (Szeged Stock Center, 1998-, Geng et al., 2000)
Alleles and Phenotypes
Causes alleles	trc^S066917a (Szeged Stock Center, 1998-, Geng et al., 2000, Deak et al., 1997)

Lethality References lethal (He et al., 2005) lethal \| embryonic stage \| recessive (Deak et al., 1997) lethal \| recessive (Szeged Stock Center, 1998-)
Sterility References
Phenotype Manifest In
	arista (He and Adler, 2002) denticle (He et al., 2005) wing hair \| P-stage \| cell autonomous \| somatic clone \| supernumerary (Geng et al., 2000) wing hair \| somatic clone \| supernumerary (Cong et al., 2001, Geng et al., 2000) wing hair \| supernumerary (He et al., 2005)
Detailed Description
Statement Reference Homozygous second instar larvae have many split denticles per segment. (He et al., 2005) Mutant cells in trc^S066917a large clones in the pupal wing often have an irregular shape, and their average cross-sectional area is ~1.6-fold larger than control neighboring cells. Hair morphogenesis is delayed in the trc^S066917a mutant cells. trc^S066917a mutants show a multiple wing hair phenotype. Expression of trc^{S292A.Scer\UAS} under the control of Scer\GAL4^ap-md544 in a trc^S066917a/+ background enhances the multiple wing hair phenotype seen when trc^S066917a is expressed in a wild-type background. (He et al., 2005) Expression of Hsap\NDRG1^Scer\UAS.cHa under the control of Scer\GAL4^Act5C.PI rescues both the lethality and the multiple wing hair phenotype of trc^S066917a mutants. (He et al., 2005) fry¹ trc^S066917a mats^e03077 triple heterozygotes have a weak multiple wing hair phenotype in 40% of wings. The split denticle phenotype of mats^e03077 trc^S066917a double mutant second instar larvae is no more severe than the split denticle phenotype of each single mutant. (He et al., 2005) Hemizygous flies have branched arista laterals. (He and Adler, 2002) trc^S066917a fry¹ double mutant clones do not have a stronger multiple wing hair phenotype (5.37 hairs/cell) than either single mutant. (Cong et al., 2001) Homozygous clones have a multiple wing hair phenotype (5.74 hairs/cell). (Cong et al., 2001) Mutant cells in homozygous clones in the wing show a strong multiple hair cell phenotype. The number of hairs per cell varies between different clones from an average of 3.5 hairs per cell to more than 7 hairs per cell. Mutant cells in pupal wings have on average a weaker phenotype than clones in adult wings. A substantial number of cells in the clone in pupal wings do not have a multiple wing hair phenotype, in contrast to adult wings. When a wing cell produces a single hair in pupal clones, the hair appears to be the same length as neighbouring wild-type hairs. Only mutant cells show the multiple hair phenotype in pupal wings containing homozygous clones. (Geng et al., 2000)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	S066917 (Levis and Hoskins R., 2012.1.10)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	37725 y^d2 w¹¹¹⁸ P{ey-FLP.N}1; P{lacW}trc^S066917a P{neoFRT}80B/TM3, P{Car20y}AS1, Ser¹
Linkouts
Comments
	The P insertion is in a negative orientation with repect to trc gene transcription (Geng et al., 2000)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}l(3)S066917^S066917 (Szeged Stock Center, 1998-, FlyBase, 1992-) P{lacW}l(3)S066917^S066917a (FlyBase, 1992-) P{lacW}trc^S066917a (FlyBase, 1992-, Geng et al., 2000, ) S066917 (Levis and Hoskins R., 2012.1.10)
Secondary FlyBase IDs

References ( 11 )
Research paper	Cooper et al., 2010, Genetics 185(3): 811--822 Molecular Genetic Analysis of Chd3 and Polytene Chromosome Region 76B-D in Drosophila melanogaster. [FBrf0211329] He et al., 2005, Mol. Biol. Cell 16(2): 689--700 The tricornered Ser/Thr protein kinase is regulated by phosphorylation and interacts with furry during Drosophila wing hair development. [FBrf0184141] He et al., 2005, Mol. Biol. Cell 16(9): 4139--4152 Drosophila mob family proteins interact with the related tricornered (trc) and warts (wts) kinases. [FBrf0188034] He and Adler, 2002, Dev. Genes Evol. 212(5): 218--229 The genetic control of arista lateral morphogenesis in Drosophila. [FBrf0148956] Cong et al., 2001, Development 128(14): 2793--2802 The furry gene of Drosophila is important for maintaining the integrity of cellular extensions during morphogenesis. [FBrf0138360] Geng et al., 2000, Genetics 156(4): 1817--1828 The tricornered Gene, which is required for the integrity of epidermal cell extensions, encodes the Drosophila nuclear DBF2-related kinase. [FBrf0131297] Deak et al., 1997, Genetics 147(4): 1697--1722 P-element insertion alleles of essential genes on the third chromosome of Drosophila melanogaster: correlation of physical and cytogenetic maps in chromosomal region 86E-87F. [FBrf0099762] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase	Levis and Hoskins R., 2012.1.10, Updated location data forDeak lines Updated location data forDeak lines [FBrf0217112]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]
Stock list	Szeged Stock Center, 1998-, Szeged Stock Center P-element collection. (Computer file) Szeged Stock Center P-element collection. (Computer file) [FBrf0111308]

Dmel\P{lacW}trcS066917a Insertion

Dmel\P{lacW}trc^S066917a Insertion