A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{lacW}Hsc70-3G0407 Insertion

General Information
Symbol Dmel\P{lacW}Hsc70-3G0407 Species D. melanogaster
Name FlyBase ID FBti0013896
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) Ecol\lacZ, Hsc70-3 Viability / fertility
Causes allele(s) Ecol\lacZHsc70-3.G0407, Hsc70-3G0407 Stock availability 1 publicly available
LINE ID P{lacW}l(1)G0407
Genomic Location
Chromosomal location X ( 10E4 ) Sequence location X:11,699,761..11,700,242 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0048823 FBti0059142 FBti0018120 FBti0071306 FBti0148790 FBti0013212 FBti0028452 FBti0054545 FBti0015664 FBti0030193 FBti0021510 FBti0011821 FBti0036768 FBti0013210 FBti0057635 FBti0036759 FBti0069164 FBti0028797 FBti0068605 FBti0068856 FBti0068997 FBti0037325 FBti0051585 FBti0013936 FBti0102440 FBti0013896 FBti0072042 FBti0111886 FBti0011918 FBti0028159 FBti0024259 FBti0013320 FBti0059344 FBti0068064 FBti0071472 FBti0048782 FBti0043589 FBti0100802
Member of Large Scale Dataset(s)
Dataset

A set of X chromosome mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or semi-lethal phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Peter et al., 2002, Bellen et al., 2011)
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Description
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What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
X:11,699,761..11,700,242 [-]
(FlyBase, 2007.6)
Orientation
Cytological location
(computed by FlyBase)
10E4 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
10E (in situ hybridization reported)
(Peter et al., 2002)
Comments concerning
location
hide Sequence Data
Flanking sequence
AJ426876
(FlyBase, 1992-, Peter et al., 2002, FlyBase, 2007.6)
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(Loewen and Feany, 2010)
Hsc70-3
(Schaefer et al., 1999.8.12, Loewen and Feany, 2010)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZHsc70-3.G0407
(Loewen and Feany, 2010)
Hsc70-3G0407
(Schaefer et al., 1999.8.12, Peter et al., 2002, Loewen and Feany, 2010)
Lethality
References
lethal | larval stage
(Peter et al., 2002)
lethal | pharate adult stage
(Peter et al., 2002)
lethal | prepupal stage
(Peter et al., 2002)
lethal | pupal stage
(Peter et al., 2002)
partially lethal - majority die | recessive
(Schaefer et al., 1999.8.12)
Sterility
References
hide Phenotype Manifest In
pupa
(Peter et al., 2002)
hide Detailed Description
Statement
Reference
Lethality occurs from the third larval instar through to the pharate adult stage. Mutant pupae are short.
(Peter et al., 2002)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
P{lacW}l(1)G0407
(FlyBase, 2007.6)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
12011
w67c23 P{lacW}Hsc70-3G0407/FM7c
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
Hsc70-3G0407
(Loewen and Feany, 2010)
P{lacW}Hsc70-3G0407
(Peter et al., 2002, FlyBase, 1992-, )
P{lacW}l(1)G0407G0407
(Schaefer et al., 1999.8.12, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Loewen and Feany, 2010, PLoS ONE 5(9): e13084
The unfolded protein response protects from tau neurotoxicity in vivo. [FBrf0211994]
Peter et al., 2002, EMBO Rep. 3(1): 34--38
Mapping and identification of essential gene functions on the X chromosome of Drosophila. [FBrf0144855]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material
Peter et al., 2002, EMBO Rpts 3(1):
Supplementary material. PX-lines.txt. [FBrf0173176]
Personal communication to FlyBase
Schaefer et al., 1999.8.12, Goettingen lethals - set 3.
Goettingen lethals - set 3. [FBrf0109189]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2007.6, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0199566]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]