Dmel\P{UAS-lacZ.Abd-B.5F24}25,2a Insertion

General Information
Symbol	Dmel\P{UAS-lacZ.Abd-B.5F24}25,2a	Species	D. melanogaster
Name		FlyBase ID	FBti0014018
Feature type	transposable_element_insertion_site
Description
Inserted element	P{UAS-lacZ.Abd-B.5F24}	Expression data
Affected gene(s)	sd	Viability / fertility
Causes allele(s)	sd^Fab-X	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	X ( 13F1-13F5 )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02	Controlled Vocabulary Terms wing blade
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation	? (Cavalli, 1999)
Cytological location (computed by FlyBase)	13F1-13F5 ( near gene of known cytology )
Cytological location (reported)	13F (in situ hybridization reported) (Cavalli, 1999)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{UAS-lacZ.Abd-B.5F24}
Location-dependent role
Size
Associated alleles	Ecol\lacZ^{Abd-B.5F24.Scer\UAS} w^Fab-7.5F24
Molecular map
Affected Gene(s)
Insertion may affect gene	sd (Bantignies et al., 2003)
Alleles and Phenotypes
Causes alleles	sd^Fab-X (Bantignies et al., 2003)

Lethality References
Sterility References
Phenotype Manifest In
	wing (Bantignies et al., 2003, Grimaud et al., 2006) wing blade (Canudas et al., 2005)
Detailed Description
Statement Reference sd^Fab-X flies show a strong crumpled wing phenotype when raised at 28.5^oC. (Grimaud et al., 2006) The wing phenotype of sd^Fab-X flies is suppressed in the following double mutant genotypes: sd^Fab-X;AGO1⁷²/AGO1⁴⁵, sd^Fab-X;Dcr-2^L811fsX, sd^Fab-X;piwi¹ and sd^Fab-X;piwi². However, this phenotype is not suppressed in the following double mutants: sd^Fab-X;spn-E^hls-E1, sd^Fab-X;Dcr-1^Q1147X/+. (Grimaud et al., 2006) 16% of homozygous females show wing blade destruction in one wing, and 72% show wing blade destruction in both wings. (Canudas et al., 2005) The wing blade destruction phenotype of homozygous sd[Fab-X] females is strongly suppressed by Bin1[R7-18]/Df(3R)sbd45: 13% of the double mutants show wing blade destruction in one wing, and 4% show wing blade destruction in both wings. The wing blade destruction phenotype of homozygous sd[Fab-X] females is significantly suppressed by Bin1[R7-18]/+ (39% show wing blade destruction in one wing, 36% show wing blade destruction in both wings), Rpd3[def24]/+ (26% show wing blade destruction in one wing, 30% show wing blade destruction in both wings) or Trl[R67]/+ (32% show wing blade destruction in one wing, 51% show wing blade destruction in both wings). (Canudas et al., 2005) The addition of Fab-7¹ suppresses the wing phenotype seen in sd^Fab-X animals. The addition of additional insertions of the P{UAS-lacZ.Abd-B.5F24} transgene (P{UAS-lacZ.Abd-B.5F3}22,51 or P{UAS-lacZ.Abd-B.5F24}21,17) represses this suppression allowing the wing phenotype to manifest again. The addition of P{Mcp-w#11}45 or P{UAS-lacZ.U/l5}1,1 does not have this effect. (Bantignies et al., 2003) Hemizygotes and heterozygotes have wild-type wing, but 95% of homozygotes have a display a destruction of the wing blade. (Bantignies et al., 2003)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	5F24 25,2 (Cavalli, 1999, Lupo et al., 2001) P{UAS-lacZ.Abd-B.5F24}25,2 (FlyBase, 1992-) P{UAS-lacZ.Abd-B.5F24}25,2a (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 6 )
Research paper	Grimaud et al., 2006, Cell 124(5): 957--971 RNAi components are required for nuclear clustering of Polycomb group response elements. [FBrf0189910] Canudas et al., 2005, Nucleic Acids Res. 33(15): 4857--4864 dSAP18 and dHDAC1 contribute to the functional regulation of the Drosophila Fab-7 element. [FBrf0188188] Bantignies et al., 2003, Genes Dev. 17(19): 2406--2420 Inheritance of Polycomb-dependent chromosomal interactions in Drosophila. [FBrf0167600] Lupo et al., 2001, Mol. Cell 7(1): 127--136 Drosophila chromosome condensation proteins Topoisomerase II and Barren colocalize with Polycomb and maintain Fab-7 PRE silencing. [FBrf0134762]
Review	Cavalli, 1999, Science 286(5441): 955--958 Epigenetic inheritance of active chromatin after removal of the main transactivator. [FBrf0112149]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]