Dmel\P{}nos^BN Insertion

General Information
Symbol	Dmel\P{}nos^BN	Species	D. melanogaster
Name		FlyBase ID	FBti0014903
Feature type	transposable_element_insertion_site
Description
Inserted element	P-element	Expression data
Affected gene(s)	nos	Viability / fertility
Causes allele(s)	nos^BN	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	3R ( 91F4 )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	91F4 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P-element
Location-dependent role
Size
Associated alleles
Molecular map
Affected Gene(s)
Insertion may affect gene	nos (Kobayashi et al., 1996, Rouget et al., 2010)
Alleles and Phenotypes
Causes alleles	nos^BN (Forbes and Lehmann, 1998, Gamberi et al., 2002, Schaner et al., 2003, Kobayashi et al., 1996, Rouget et al., 2010)

Lethality References
Sterility References
Phenotype Manifest In
	abdominal segment (Curtis et al., 1995, Becalska et al., 2011) dorsal closure embryo (Gamberi et al., 2002) embryonic abdominal segment (Verrotti and Wharton, 2000, Yu et al., 2010) germline cell (Deshpande et al., 1999) pole cell (Kobayashi et al., 1996, Asaoka-Taguchi et al., 1999) pole cell \| embryonic stage 12 \| maternal effect (Sato et al., 2007) pole cell \| embryonic stage 13 \| maternal effect (Sato et al., 2007) pole cell \| embryonic stage 14 \| maternal effect (Sato et al., 2007) pole cell \| embryonic stage 15 \| maternal effect (Sato et al., 2007) pole cell \| embryonic stage 16 \| maternal effect (Sato et al., 2007) pole cell \| maternal effect (Hayashi et al., 2004)
Detailed Description
Statement Reference nos[BN],nos[+1+3.T:MS2\MCP.BS]/nos[BN] embryos display a reduction in abdominal segmentation, forming an average of 6 of the 8 abdominal segments characteristic of wild-type embryos. (Becalska et al., 2011) nos[BN],nos[+1+3.T:MS2\MCP.BS]/nos[BN] embryos carrying a Df(2L)BSC32/+ mutation display a reduction in abdominal segmentation, forming an average of 2.4 abdominal segments. nos[BN],nos[+1+3.T:MS2\MCP.BS]/nos[BN] embryos carrying a aub[QC42]/+ mutation display a reduction in abdominal segmentation, forming an average of 2-3 abdominal segments. Mutant embryos display the normal number of pole cells. nos[BN],nos[+1+3.T:MS2\MCP.BS]/nos[BN] embryos carrying a aub[HN]/+ mutation display a reduction in abdominal segmentation, forming an average of 3-4 abdominal segments. nos[BN],nos[+1+3.T:MS2\MCP.BS]/nos[BN] embryos carrying a squ[HE47]/+ mutation do not display a reduction in abdominal segmentation compared to nos[BN],nos[+1+3.T:MS2\MCP.BS]/nos[BN] embryos. rump[1] mutant embryos carrying a nos[BN], nos[+1+3.T:MS2\MCP.BS]/nos[BN] mutation display an increase in the number of embryos that develop less than 8 abdominal segments. rump[1] mutant embryos carrying an aub[QC42]/+ mutation, and the mutation nos[BN], nos[+1+3.T:MS2\MCP.BS]/nos[BN] , display an increase in the number of embryos that develop less than 8 abdominal segments. (Becalska et al., 2011) wah[+t9.9] rescues the reduced abdominal segments phenotype seen in embryos produced by nos[ΔBX], nos[BN]/nos[BN] females that are also heterozygous for wah[1]. (Yu et al., 2010) nos[ΔBX], nos[BN]/nos[BN] females produce embryos than have a reduction in the average number of abdominal segments. nos[BN] homozygous ovaries are superficially normal. nos[BN] females are considerably less fecund than wild type, but nevertheless produce eggs continuously over an 11-day interval. (Yu et al., 2010) nos[ΔBX], nos[BN]/nos[BN] females that are also heterozygous for wah[1] give rise to embryos that rarely hatch, concomitant with a reduction in the average number of abdominal segments from 3.7 to 1.9. nos[ΔBX], nos[BN]/nos[BN] females that are also heterozygous for wah[06536], wah[c04892] or Df(3R)Exel8162 give rise to embryos with a reduction in the average number of abdominal segments. wah[1] nos[BN]/nos[BN] females appear to be significantly less fertile than nos[BN]/nos[BN] females. In contrast to nos[BN] homozygous ovaries, ovaries from wah[1] nos[BN]/nos[BN] females have few normal ovarioles: many contain either no maturing egg chambers or only a single mature stage 13/14 oocyte; and many contain aberrant, small egg chambers that contain only one or two highly polyploid germ line nuclei. In contrast to nos[BN]/nos[BN] females, wah[1], nos[BN]/nos[BN] females produce eggs for only 5-6 days after eclosion. wah[06536], nos[BN]/nos[BN] females exhibit more than tenfold lower fecundity between days 9 and 13 than between days 3 and 8. wah[c04892], nos[BN]/nos[BN] females exhibit more than tenfold lower fecundity between days 9 and 13 than between days 3 and 8. wah[1], nos[BN]/nos[L7] females do not exhibit age-dependent loss of fecundity. A single copy of wah[+t9.9] can rescue the age-dependent female infertility of wah[1], nos[BN]/nos[BN] females. However, wah[1], nos[BN] homozygotes expressing a single copy of wah[+t9.9] exhibit age-dependent infertility, producing no eggs after the 8th day post-eclosion. In ovarioles from very young wah[1], nos[BN]/nos[BN] flies, the spectrosomes of germline stem cells and cystoblasts and the fusomes of immature cysts appear normal. But slightly older ovaries are bereft of germline cells: some germaria contain only one or two germline cysts and many have none. In these older ovaries, differentiation or development of the few remaining cysts is abnormal, many of which contain one or two large polyploid cells. Doubly homozygous mof[1]; nos[BN] females are nearly as prolific as nos[BN] controls 3-8 days post-eclosion. However, their fecundity drops dramatically 9-13 days post-eclosion. (Yu et al., 2010) Heterozygous embryos do not show segmentation defects. (Jain and Gavis, 2008) The frequency and severity of abdominal segmentation defects in embryos derived from rump¹ homozygous females is increased by one copy of nos^BN. Abdominal segments 4 and 5 are most frequently affected. The ability of nos^nos.+2.Hsp83 to rescue abdominal segmentation of nos^BN embryos is severely compromised in a rump¹/Df(3R)by416 background; more than 90% of the embryos develop fewer than 4 abdominal segments. (Jain and Gavis, 2008) Approximately 19% of pole cells in embryos derived from nos[BN] females are undergoing apoptosis at stage 12-14. Around 33% of pole cells are undergoing apoptosis at stage 14-16. (Sato et al., 2007) Removal of zygotic W (W[05014]/Df(3L)H99) suppresses the pole cell apoptosis seen in embryos derived from nos[BN] females. Approximately 7% of pole cells examined at stage 12-14 are undergoing apoptosis. Expression of W[Scer\UAS.P\T.T:Zzzz\FLAG] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] enhances the pole cell apoptosis seen in stage 14-16 embryos derived from nos[BN] females. Expression of Tao-1[D168A.Scer\UAS.P\T.T:Zzzz\FLAG] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] suppresses the pole cell apoptosis seen in stage 13-16 embryos derived from nos[BN] females. Expression of Tao-1[Scer\UAS.P\T.T:Zzzz\FLAG] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] enhances the pole cell apoptosis seen in stage 13-16 embryos derived from nos[BN] females. Expression of skl[Scer\UAS.P\T.cSa] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] enhances the pole cell apoptosis seen in stage 13-16 embryos derived from nos[BN] females. Removal of zygotic W (W[05014]/Df(3L)H99) suppresses the pole cell apoptosis seen when Tao-1[Scer\UAS.P\T.T:Zzzz\FLAG] is expressed under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] in stage 13-16 embryos derived from nos[BN] females. Removal of zygotic W (W[05014]/Df(3L)H99) suppresses the pole cell apoptosis seen when skl[Scer\UAS.P\T.cSa] is expressed under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] in stage 13-16 embryos derived from nos[BN] females. (Sato et al., 2007) Only about 55% of the progeny of hb¹⁵; nos^BN germ-line clone mothers mated to wild-type fathers hatch into first instar larvae, but most of those that hatch make it to adulthood (42% of all progeny). This embryonic lethality is rescued by nos^+t5.7. (Deshpande et al., 2005) Homozygous Df(3L)H99 embryos that are derived from nos^BN homozygous females show a suppression of the apoptosis of the pole cells that is seen in embryos derived from nos^BN homozygous females mated to wild-type males. These rescued "pole cells" (from homozygous Df(3L)H99 embryos that are derived from nos^BN homozygous females) can become integrated into somatic tissues, such as midgut epithelium, tracheal epithelium and gastric caeca, when transplanted into host embryos (this is not seen with either control pole cells or pole cells derived from nos^BN homozygous females mated to wild-type males). The pole cells that are integrated into somatic tissues are morphologically indistinguishable from their neighbouring host cells. In addition, the rescued "pole cells" are incorporated into gonads in approximately 25% of cases, when transplanted into host embryos (this phenotype is not seen when pole cells derived from nos^BN homozygous females mated to wild-type males are transplanted into host embryos). These pole cells are morphologically indistinguishable from the host pole cells, but do not contribute to egg or sperm production. (Hayashi et al., 2004) Beginning at stage 9/10, most pole cells are lost in embryos derived from homozygous females. The pole cells in these embryos sometimes show irregular shapes characteristic of apoptotic cells and 19.7% are TUNEL-positive. (Hayashi et al., 2004) Homozygotes show severe head involution defects in only 4% of larvae. (Gamberi et al., 2002) Hmag\nos1^nos.UTR fails to rescue the abdominal defects of embryos derived from nos^BN females. (Mochizuki et al., 2000) No genetic interaction in terms of abdominal segmentation is seen in the cup^unspecified/+ ; nos^BN combination. No genetic interaction in terms of abdominal segmentation is seen in the cup^unspecified/nos^ΔBX ; nos^BN combination. (Verrotti and Wharton, 2000) The average number of pole cells per embryo is reduced compared to controls in embryos derived from homozygous females. (Asaoka-Taguchi et al., 1999) When embryos are also mutant for Sxl^fP7B0, the germ cells form two clusters instead of one. (Deshpande et al., 1999) Germ cells clump prematurely and stay in the middle of the embryo. (Deshpande et al., 1999) The effects of loss of maternal and zygotic nos product on germ cell migration is studied in females with hb¹⁵ nos^BN mutant germ line clones crossed to nos¹⁸/Df(3R)Dl-FX3 males. Germ cells are formed in the embryos indicating nos function is not required for their formation. nos activity is essential for germ cell migration, from stage 10 onwards. Following exit of the germ cells of the posterior midgut pocket, germ cells fail to migrate over the surface of the gut and instead cluster tightly together on the outer gut surface. Mutant germ cells are of varying size and often have an irregular surface. Zygotic nos expression cannot compensate for the loss of maternal nos. The few germ cells that appear to associate with the somatic embryonic gonad are unable to incorporate into the adult gonad even in the presence of a zygotically active copy of the gene. (Forbes and Lehmann, 1998) Pole cells fail to migrate into the gonads. Transplantation of pole cells into hosts that can form normal abdomens demonstrates the pole cells cannot penetrate the gonads. No ovo^D1 females transplanted with nos^BN pole cells produce progeny, also demonstrating the pole cells cannot penetrate the gonads. (Kobayashi et al., 1996) Two copies of P{Dvnos} partially rescues the mutant phenotype. (Curtis et al., 1995) One copy of P{Dmnos} is sufficient to completely rescue the nos^L7 abdominal segmentation phenotype. (Curtis et al., 1995)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{}nos^BN (Kobayashi et al., 1996, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 17 )
Research paper	Becalska et al., 2011, Dev. Biol. 349(1): 46--52 Aubergine is a component of a nanos mRNA localization complex. [FBrf0212348] Rouget et al., 2010, Nature 467(7319): 1128--1132 Maternal mRNA deadenylation and decay by the piRNA pathway in the early Drosophila embryo. [FBrf0212183] Yu et al., 2010, genesis 48(3): 161--170 E(nos)/CG4699 required for nanos function in the female germ line of Drosophila. [FBrf0210233] Jain and Gavis, 2008, Development 135(5): 973--982 The Drosophila hnRNP M homolog Rumpelstiltskin regulates nanos mRNA localization. [FBrf0202711] Sato et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104(18): 7455--7460 Maternal Nanos represses hid/skl-dependent apoptosis to maintain the germ line in Drosophila embryos. [FBrf0200950] Deshpande et al., 2005, Mech. Dev. 122(5): 645--657 Nanos downregulates transcription and modulates CTD phosphorylation in the soma of early Drosophila embryos. [FBrf0187981] Hayashi et al., 2004, Proc. Natl. Acad. Sci. U.S.A. 101(28): 10338--10342 Nanos suppresses somatic cell fate in Drosophila germ line. [FBrf0179243] Schaner et al., 2003, Dev. Cell 5(5): 747--757 A conserved chromatin architecture marks and maintains the restricted germ cell lineage in worms and flies. [FBrf0167477] Gamberi et al., 2002, Development 129(11): 2699--2710 An anterior function for the Drosophila posterior determinant Pumilio. [FBrf0148963] Mochizuki et al., 2000, Dev. Genes Evol. 210(12): 591--602 Expression and evolutionary conservation of nanos-related genes in Hydra. [FBrf0135230] Verrotti and Wharton, 2000, Development 127(23): 5225--5232 Nanos interacts with Cup in the female germline of Drosophila. [FBrf0131417] Asaoka-Taguchi et al., 1999, Nat. Cell Biol. 1(7): 431--437 Maternal Pumilio acts together with Nanos in germline development in Drosophila embryos. [FBrf0111783] Deshpande et al., 1999, Cell 99(3): 271--281 Novel functions of nanos in downregulating mitosis and transcription during the development of the Drosophila germline. [FBrf0111849] Forbes and Lehmann, 1998, Development 125(4): 679--690 Nanos and Pumilio have critical roles in the development and function of Drosophila germline stem cells. [FBrf0101903] Kobayashi et al., 1996, Nature 380(6576): 708--711 Essential role of the posterior morphogen nanos for germline development in Drosophila. [FBrf0087514] Curtis et al., 1995, Development 121(6): 1899--1910 nanos is an evolutionarily conserved organizer of anterior-posterior polarity. [FBrf0078260]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{}nosBN Insertion

Dmel\P{}nos^BN Insertion