A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{PZ}CenG1ArN149 Insertion

General Information
Symbol Dmel\P{PZ}CenG1ArN149 Species D. melanogaster
Name FlyBase ID FBti0015387
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data
Affected gene(s) CenG1A Viability / fertility viable
Causes allele(s) CenG1ArN149 Stock availability none publicly available
LINE ID n(2)rN149
Genomic Location
Chromosomal location 2L ( 34E1 ) Sequence location 2L:13,878,121..13,878,121 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0152143 FBti0048987 FBti0026044 FBti0015387 FBti0008076 FBti0112706 FBti0034246 FBti0028361 FBti0034252 FBti0103274 FBti0054570 FBti0105069 FBti0057539 FBti0018782 FBti0102806 FBti0110656 FBti0124787 FBti0112189 FBti0109644 FBti0008681 FBti0104161 FBti0105272 FBti0027067 FBti0050782 FBti0045940 FBti0049592 FBti0055055
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
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FB2013_03
References
Spradling et al., 1999, Genetics 153(1): 135--177
FB2013_02
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:13,878,121..13,878,121 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
34E1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026444
(FlyBase, 1992-)
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
CenG1A
(Ashburner et al., 1999)
hide Alleles and Phenotypes
Causes alleles
CenG1ArN149
(Ashburner et al., 1999, Ashburner et al., 1999.10.12)
Lethality
References
viable
(Ashburner et al., 1999)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
No obvious phenotype.
(Ashburner et al., 1999)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
n(2)rN149
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
Location 2L:13878121-13878122 confirmed by FlyBase alignment of dbGSS accession AQ026444 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{PZ}BG:DS08220.1rN149
(Ashburner et al., 1999, FlyBase, 1992-)
P{PZ}cenG1ArN149
(FlyBase, 1992-, FlyBase, 2005, )
P{PZ}CenG1ArN149
 
P{PZ}rN149
(Ashburner et al., 1999)
Secondary FlyBase IDs
hide References ( 11 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Supplementary material
Ashburner et al., 1999, Genetics 153(1):
Table S1. P-element insertions in the Adh region. [FBrf0112150]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
Roote, 2000.6.13, New P derivatives.
New P derivatives. [FBrf0129261]
Ashburner et al., 1999.10.12, Adh.
Adh. [FBrf0112153]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]