Dmel\P{lacW}G0280a Insertion

General Information
Symbol	Dmel\P{lacW}G0280a	Species	D. melanogaster
Name		FlyBase ID	FBti0015480
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	tyn	Viability / fertility
Causes allele(s)		Stock availability	2 publicly available
LINE ID	P{lacW}l(1)G0280
Genomic Location
Chromosomal location	X ( 1A1 )	Sequence location	X:72,384..72,752 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{lacW}.G A set of X chromosome mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or semi-lethal phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800). Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Peter et al., 2002, Bellen et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	X:72,384..72,752 [+] (FlyBase, 2007.6)
Orientation	? (Schaefer et al., 1999.12.14)
Cytological location (computed by FlyBase)	1A1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	1A (in situ hybridization reported) (Schaefer et al., 1999.12.14) 1B1--1B4 (in situ hybridization reported) (Peter et al., 2002)
Comments concerning location
Sequence Data
Flanking sequence	AJ299990 (Peter et al., 2002, FlyBase, 2007.6)
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	tyn (Levis, 2002.1.3)
Alleles and Phenotypes
Causes alleles

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	P{lacW}l(1)G0280 (FlyBase, 2007.6)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	12291 P{lacW}G0280a w^67c23 P{lacW}G0280b, l(1)G0280^G0280/FM7c
Kyoto	111940 P{lacW}G0280a P{lacW}G0280b, l(1)G0280^G0280 w^* P{neoFRT}19A/FM7c; P{ey-FLP.N}5
Linkouts
Comments
	P{lacW}G0280a maps 90bp upstream of the 5\' end of the SP71 gene. (Levis, 2002.1.3) See: l(1)G0280^G0280; not known which insertion (if either) is responsible for lethal phenotype. (Schaefer et al., 1999.12.14)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}G0280a (Levis, 2002.1.3, Peter et al., 2002, FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 9 )
Research paper	Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Peter et al., 2002, EMBO Rep. 3(1): 34--38 Mapping and identification of essential gene functions on the X chromosome of Drosophila. [FBrf0144855] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Peter et al., 2002, EMBO Rpts 3(1): Supplementary material. PX-lines.txt. [FBrf0173176]
Personal communication to FlyBase	Levis, 2002.1.3, Flanking sequence for P{lacW}G0280a - Correction. Flanking sequence for P{lacW}G0280a - Correction. [FBrf0141835] Schaefer et al., 1999.12.14, Goettingen lethals - set 6. Goettingen lethals - set 6. [FBrf0125056]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2007.6, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0199566] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]