Dmel\P{lacW}disp^S037707 Insertion

General Information
Symbol	Dmel\P{lacW}disp^S037707	Species	D. melanogaster
Name		FlyBase ID	FBti0015544
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	disp	Viability / fertility
Causes allele(s)	disp^S037707	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	3R ( 83C5 )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	83C5 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	disp (Burke et al., 1999)
Alleles and Phenotypes
Causes alleles	disp^S037707 (Burke et al., 1999, Gallet et al., 2003)

Lethality References lethal \| embryonic stage \| recessive \| rescuable maternal effect \| segment polarity expression pattern (Burke et al., 1999) lethal \| pupal stage \| recessive (Burke et al., 1999)
Sterility References
Phenotype Manifest In
	1st posterior cell \| somatic clone (Burke et al., 1999) denticle \| germline clone \| maternal effect (Gallet et al., 2003) denticle belt (Burke et al., 1999) embryonic/first instar larval cuticle \| germline clone (Gallet et al., 2006) embryonic/first instar larval cuticle \| germline clone \| maternal effect (Gallet et al., 2003) embryonic/first instar larval cuticle \| ventral (Burke et al., 1999) wing \| somatic clone (Burke et al., 1999)
Detailed Description
Statement Reference First instar larvae in which disp^S037707 germline clones have been induced exhibit only type 4 cuticle cells. (Gallet et al., 2006) Expression of hh^Scer\UAS.cIa, under the control of Scer\GAL4^unspecified in disp^S037707 mutant embryos rescues some type 1 cuticle, but no type 2 or type 1 cuticle is formed. (Gallet et al., 2006) Expression of hh^Scer\UAS.cIa with Scer\GAL4^en-e16E in disp^S037707 germ-line clone embryos doesn\'t rescue naked cuticle formation, but does increase denticle diversity in the ventral cuticle: row 3,4 and 5 type denticles are all present. Expression of hh^{N.Scer\UAS.cGa} with Scer\GAL4^en-e16E in disp^S037707 germ-line clone embryos doesn\'t rescue naked cuticle formation, but does increase denticle diversity in the ventral cuticle: row 3,4 and 5 type denticles are all present: denticles type 3 and 4 cover each segment, whereas type 5 denticles have almost totally disappeared. (Gallet et al., 2003) The ventral cuticles of disp^S037707 germ-line clone embryos lack naked cuticle, instead forming a lawn of small denticles different from row 5 type. (Gallet et al., 2003) The overgrowth of the anterior compartment of the wing disc seen in larvae expressing hh^{N.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^en-e16E in a hh^ts2 background is still seen if the discs are also carrying disp^S037707. The ability of hh^{SF.Scer\UAS.T:Rnor\CD2} to rescue the growth defects of hh^ts2 wing imaginal discs when expressed under the control of Scer\GAL4^en-e16E is unaffected if the discs are also homozygous for disp^S037707. (Burke et al., 1999) Homozygotes lacking zygotic disp function survive until early pupal stages. Animals lacking both maternal and zygotic disp function die during embryogenesis with a strong segment-polarity phenotype; the embryos display a lawn of denticle belts and fail to secrete ventral cuticle. The embryos are rescued by a disp⁺ paternal chromosome. Homozygous clones in the wing can contribute to wild-type wing margin structures. Large clones in the posterior compartment cause a significant reduction in the distance between veins L3 and L4. The wing is reduced in size, but otherwise patterning remains normal. (Burke et al., 1999) disp^{Scer\UAS.T:Ivir\HA1} rescues disp^S037707 animals to adulthood when expressed under the control of Scer\GAL4^en-e16E. The rescued flies have normal patterning in the wing, leg, notum and abdomen and give rise to viable offspring, but have a dramatic reduction in eye size. (Burke et al., 1999)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}disp^S037707 (Burke et al., 1999, FlyBase, 1992-) P{lacW}l(3)S037707^S037707 (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 5 )
Research paper	Gallet et al., 2006, Development 133(3): 407--418 Cholesterol modification is necessary for controlled planar long-range activity of Hedgehog in Drosophila epithelia. [FBrf0190290] Gallet et al., 2003, Dev. Cell 4(2): 191--204 Cholesterol modification of hedgehog is required for trafficking and movement, revealing an asymmetric cellular response to hedgehog. [FBrf0155685] Burke et al., 1999, Cell 99(7): 803--815 Dispatched, a novel sterol-sensing domain protein dedicated to the release of cholesterol-modified hedgehog from signaling cells. [FBrf0122981] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacW}dispS037707 Insertion

Dmel\P{lacW}disp^S037707 Insertion