A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}endoAEP593 Insertion

General Information
Symbol Dmel\P{EP}endoAEP593 Species D. melanogaster
Name FlyBase ID FBti0016445
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) endoA Viability / fertility
Causes allele(s) endoAEP593 Stock availability none publicly available
LINE ID EP(3)0593
Genomic Location
Chromosomal location 3R ( 91D4 ) Sequence location 3R:14,732,374..14,732,374 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0053443 FBti0105986 FBti0108573 FBti0100158 FBti0074674 FBti0030249 FBti0036194 FBti0016445 FBti0029057 FBti0107142 FBti0016437 FBti0027160 FBti0011617 FBti0011320 FBti0057287 FBti0051163 FBti0126160 FBti0052527 FBti0113035 FBti0104422 FBti0057794 FBti0021483 FBti0110024 FBti0043242 FBti0021843
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
3
(BDGP Project Members, 1994-1999)
Sequence Location
3R:14,732,374..14,732,374 [+]
(FlyBase, 2005)
Orientation
Cytological location
(computed by FlyBase)
91D4 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
91D4-91D4 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ254591
(FlyBase, 1992-)
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
endoA
(BDGP Project Members, 2000-)
hide Alleles and Phenotypes
Causes alleles
endoAEP593
(Gene Disruption Project members, 2001-, Verstreken et al., 2002)
Lethality
References
lethal | larval stage | recessive
(Verstreken et al., 2002)
lethal | pupal stage
(Verstreken et al., 2002)
viable
(Verstreken et al., 2002)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Homozygous mutants die during the third instar larval stage. endoAEP593/endoAEP927 and endoAEP593/endoAEP464 transheterozygotes die as pupae. endoAEP3502/endoAEP593 transheterozygotes are viable.
(Verstreken et al., 2002)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(3)0593
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
Location 3R:14732373-14732374 determined by FlyBase alignment of dbGSS accession AQ254591 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EP(3)0593
(FlyBase, 1992-)
P{EP}endoAEP593
(FlyBase, 2005, FlyBase, 1992-, )
P{EP}endophilinEP593
(FlyBase, 1992-)
P{EP}EP0593
(FlyBase, 1992-)
P{EP}P(EP0593)
(Verstreken et al., 2002)
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Verstreken et al., 2002, Cell 109(1): 101--112
Endophilin mutations block clathrin-mediated endocytosis but not neurotransmitter release. [FBrf0146915]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]