Dmel\P{EP}Pdk1^EP837 Insertion

General Information
Symbol	Dmel\P{EP}Pdk1^EP837	Species	D. melanogaster
Name		FlyBase ID	FBti0016500
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	Pdk1	Viability / fertility
Causes allele(s)	Pdk1³, Pdk1⁴, Pdk1⁵, Pdk1^EP837	Stock availability	none publicly available
LINE ID	EP(3)0837
Genomic Location
Chromosomal location	3L ( 61B1 )	Sequence location	3L:131,762..131,762 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)	3 (BDGP Project Members, 1994-1999)
Sequence Location	3L:131,762..131,762 [+] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	61B1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	61A4-61A4 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ254649 (FlyBase, 1992-)
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Pdk1 (BDGP Project Members, 2000-, Jones et al., 2009, Eddison et al., 2011)
Alleles and Phenotypes
Causes alleles	Pdk1³ (Rintelen et al., 2001) Pdk1⁴ (Rintelen et al., 2001) Pdk1⁵ (Rintelen et al., 2001) Pdk1^EP837 (Cho et al., 2001, Radimerski et al., 2002, Rintelen et al., 2001, Gene Disruption Project members, 2001-, Reiling et al., 2005, Reiling and Hafen, 2004, Jones et al., 2009, Eddison et al., 2011)

Lethality References lethal (Rintelen et al., 2001) lethal \| recessive (Rintelen et al., 2001) viable (Roote, 2003.9.11) viable \| somatic clone (Rintelen et al., 2001)
Sterility References male sterile (Roote, 2003.9.11, Rintelen et al., 2001)
Phenotype Manifest In
	adult head (Roote, 2003.9.11) eye (Cho et al., 2001, Rintelen et al., 2001) ommatidium (Cho et al., 2001) wing (Cho et al., 2001) wing disc \| somatic clone (Scott et al., 2007)
Detailed Description
Statement Reference Neuronal expression of Pdk1[EP837] under the control of Scer\GAL4[elav-C155] increases sensitivity to ethanol sedation. (Eddison et al., 2011) Age-related locomotor impairment is significantly delayed in mutant males compared to controls. Mutant males show increased resistance to paraquat compared to controls. (Jones et al., 2009) Homozygous clones in the wing disc are reduced in size by 78% compared to their wild-type twin spot clones. (Scott et al., 2007) The reduced size of Pk61C[5] clones in the wing disc is not altered if the clones are also homozygous for Atg1[Δ3D]. (Scott et al., 2007) Animals expressing both Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF have a large-eye phenotype. Expression of Lk6^EP30.18 in this background strongly suppresses this phenotype. (Reiling et al., 2005) Flies co-expressing Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF have a large eye phenotype. The large eye phenotype caused by co-expression of Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of scyl^EP22.1. The large eye phenotype caused by co-expression of Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of chrb^EP1035. The large eye phenotype caused by co-expression of Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF is suppressed nearly to wild type by simultaneous co-expression of both scyl^EP22.1 and chrb^EP1035. (Reiling and Hafen, 2004) Pk61C⁴/Pk61C⁵ surviving flies have reduced heads. (Roote, 2003.9.11) Co-expression of Pk61C^EP837 enhances the bent wing phenotype caused by expression of S6k^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544. (Radimerski et al., 2002) Expression of Pk61C^EP837 under the control of Scer\GAL4^ap-md544 has no effect on the wing. (Radimerski et al., 2002) When Pk61C^EP837 and Akt1^{Scer\UAS.T:Ivir\HA1} are coexpressed under the control of Scer\GAL4^GMR.PF, the eyes display a severely crumpled morphology. The eye bristles are enlarged even more severely, and the boundaries of all ommatidia and photoreceptor cells disappear. The addition of Pka-R1^BDK.Scer\UAS to Pk61C^EP837, Akt1^{Scer\UAS.T:Ivir\HA1} flies has no effect on the eye phenotype. (Cho et al., 2001) Expression of Pk61C^EP837 when driven by Scer\GAL4^GMR.PF causes an increase in ommatidial size (about 1.33 fold bigger than wild-type). When driven by Scer\GAL4^ap-md544 the wing is convex towards the dorsal side. (Cho et al., 2001) Coexpression of Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF causes a substantial increase in eye size. Coexpression of Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PB in clones of cells in the eye results in a cell autonomous increase in cell size. (Rintelen et al., 2001) Expression of Pk61C^EP837 under the control of Scer\GAL4^GMR.PF results in a slight but significant increase in eye size. Expression of Pk61C^EP837 under the control of Scer\GAL4^GMR.PB in clones of cells in the eye does not increase cell size. (Rintelen et al., 2001) The heads of mosaic flies with homozygous heads (generated using the "ey-FLP" system) are reduced in size. (Rintelen et al., 2001) Pten^dj189/Pten^c494 ; Pk61C⁴/Pk61C⁵ animals can survive to adulthood, although the abdomen is unproportionally reduced in size and leg structures are deformed. (Rintelen et al., 2001) Homozygous Pk61C⁵ larvae and transheterozygous Pk61C⁵/Pk61C^EP3091 larvae die during the second instar. Pk61C⁵/Pk61C⁴ flies are delayed in development for one day, smaller than normal and have an 18% reduction in body weight. This reduction in size and weight is primarily due to a decrease in cell size. Pk61C⁵/Pk61C⁴ male flies are almost completely sterile, although they show no obvious defect in sperm morphology or in mating behaviour. Photoreceptor cells within homozygous clones in the eye are about 30% smaller than heterozygous cells outside the clone. This phenotype is cell autonomous. The heads of mosaic flies with homozygous heads (generated using the "ey-FLP" system) are reduced in size. (Rintelen et al., 2001) Pk61C⁵/Pk61C⁴ flies are delayed in development for one day, smaller than normal and have an 18% reduction in body weight. This reduction in size and weight is primarily due to a decrease in cell size. Pk61C⁵/Pk61C⁴ male flies are almost completely sterile, although they show no obvious defect in sperm morphology or in mating behaviour. The heads of mosaic flies with homozygous heads (generated using the "ey-FLP" system) are reduced in size. (Rintelen et al., 2001)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(3)0837 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	Location 3L:115294-115295 confirmed by FlyBase alignment of dbGSS accession AQ254649 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP837 (Reiling and Hafen, 2004, Jones et al., 2009) EP837^PDK1 (Eddison et al., 2011) EP(3)387 (Radimerski et al., 2002) EP(3)0837 (Rintelen et al., 2001, FlyBase, 1992-) EPPDK1 (Reiling et al., 2005) P{EP}EP0837 (FlyBase, 1992-) P{EP}Pdk1^EP837 P{EP}Pk61C^EP0837 (FlyBase, 1992-) P{EP}Pk61C^EP837 (FlyBase, 1992-, FlyBase, 2005) UAS-dPDK1 (Radimerski et al., 2002)
Secondary FlyBase IDs

References ( 17 )
Research paper	Eddison et al., 2011, Neuron 70(5): 979--990 arouser Reveals a Role for Synapse Number in the Regulation of Ethanol Sensitivity. [FBrf0213908] Jones et al., 2009, Exp. Gerontol. 44(8): 532--540 A forward genetic screen in Drosophila implicates insulin signaling in age-related locomotor impairment. [FBrf0208462] Scott et al., 2007, Curr. Biol. 17(1): 1--11 Direct induction of autophagy by Atg1 inhibits cell growth and induces apoptotic cell death. [FBrf0194462] Reiling et al., 2005, Curr. Biol. 15(1): 24--30 Diet-dependent effects of the Drosophila Mnk1/Mnk2 homolog Lk6 on growth via eIF4E. [FBrf0183826] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Reiling and Hafen, 2004, Genes Dev. 18(23): 2879--2892 The hypoxia-induced paralogs Scylla and Charybdis inhibit growth by down-regulating S6K activity upstream of TSC in Drosophila. [FBrf0180258] Radimerski et al., 2002, Nat. Cell Biol. 4(3): 251--255 dS6K-regulated cell growth is dPKB/dPI(3)K-independent, but requires dPDK1. [FBrf0145164] Cho et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98(11): 6144--6149 Drosophila phosphoinositide-dependent kinase-1 regulates apoptosis and growth via the phosphoinositide 3-kinase-dependent signaling pathway. [FBrf0137097] Rintelen et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98(26): 15020--15025 PDK1 regulates growth through Akt and S6K in Drosophila. [FBrf0141724] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase	Roote, 2003.9.11, Df(3L)ED4079. Df(3L)ED4079. [FBrf0161607] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}Pdk1EP837 Insertion

Dmel\P{EP}Pdk1^EP837 Insertion