Dmel\P{EP}tau^EP3597 Insertion

General Information
Symbol	Dmel\P{EP}tau^EP3597	Species	D. melanogaster
Name		FlyBase ID	FBti0016569
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	RpS10a, tau	Viability / fertility
Causes allele(s)	tau^EP3597	Stock availability	none publicly available
LINE ID	EP(3)3597
Genomic Location
Chromosomal location	3R ( 98A14 )	Sequence location	3R:23,478,147..23,478,315 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	3 (BDGP Project Members, 1994-1999)
Sequence Location	3R:23,478,147..23,478,315 [+] (FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)	98A14 ( inferred by FlyBase from sequence location )
Cytological location (reported)	98A6-98A6 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ254739 (FlyBase, 1992-)
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	RpS10a (BDGP Project Members, 2000-, Doerflinger et al., 2003) tau (Doerflinger et al., 2003, Yeh et al., 2010)
Alleles and Phenotypes
Causes alleles	tau^EP3597 (Doerflinger et al., 2003, Yeh et al., 2010)

Lethality References viable (Doerflinger et al., 2003)
Sterility References fertile (Doerflinger et al., 2003)
Phenotype Manifest In

Detailed Description
Statement Reference A heterozygous tau[EP3597] background reduced the bristle loss phenotype found upon overexpression of Hsap\MAPT[Scer\UAS.cWa] under the control of Scer\GAL4[Eq1]. (Yeh et al., 2010) Homozygotes have no obvious morphological or behavioural defects. The organisation of microtubules in the follicle and germline cells is indistinguishable from wild type. (Doerflinger et al., 2003)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(3)3597 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	Location 3R:23478145-23478315 determined by FlyBase alignment of dbGSS accession AQ254739 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP(3)3597 (Doerflinger et al., 2003, FlyBase, 1992-) P{EP}EP3597 (FlyBase, 1992-) P{EP}tau^EP3597 (Doerflinger et al., 2003, FlyBase, 2005, FlyBase, 1992-, ) tau^EP3597 (Yeh et al., 2010)
Secondary FlyBase IDs

References ( 10 )
Research paper	Yeh et al., 2010, Biochem. Biophys. Res. Commun. 391(1): 510--516 Drosophila notal bristle as a novel assessment tool for pathogenic study of Tau toxicity and screening of therapeutic compounds. [FBrf0209785] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Doerflinger et al., 2003, Development 130(17): 3965--3975 The role of PAR-1 in regulating the polarised microtubule cytoskeleton in the Drosophila follicular epithelium. [FBrf0160491] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}tauEP3597 Insertion

Dmel\P{EP}tau^EP3597 Insertion