Dmel\P{lacW}krz^S047819 Insertion

General Information
Symbol	Dmel\P{lacW}krz^S047819	Species	D. melanogaster
Name		FlyBase ID	FBti0016838
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, krz	Viability / fertility
Causes allele(s)	Ecol\lacZ^krz-S047819, krz^S047819	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	3R ( 100E3 )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	100E3 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Roman et al., 2000) krz (Roman et al., 2000)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^krz-S047819 (Roman et al., 2000) krz^S047819 (Roman et al., 2000)

Lethality References lethal (Kyriakakis et al., 2008) lethal \| embryonic stage (Tipping et al., 2010) lethal \| recessive (Roman et al., 2000, Mukherjee et al., 2005)
Sterility References sterile (Molnar et al., 2011)
Phenotype Manifest In
	embryonic/first instar larval cuticle (Tipping et al., 2010) embryonic/larval fat body (Roman et al., 2000) imaginal disc \| third instar larval stage (Molnar et al., 2011) lamellocyte (Roman et al., 2000) melanotic mass \| ectopic (Roman et al., 2000) presumptive embryonic/larval nervous system \| embryonic stage 15 (Tipping et al., 2010)
Detailed Description
Statement Reference Expression of krz[Scer\UAS.cMa] in the central nervous system under the control of Scer\GAL4[wor.PA] rescues the lethality of krz[d1]/krz[S047819]. The surviving flies are sterile and display slightly folded wings. Wing discs in rescued larvae have a normal appearance. (Molnar et al., 2011) krz[d1]/krz[S047819] mutant animals survive until the third larval instar, where they become immobile and flaccid and form melanotic tumors. The imaginal discs of these mutant larvae are very reduced in size and display increased cell death. Expression of krz[Scer\UAS.cMa] in the central nervous system under the control of Scer\GAL4[wor.PA] rescues the lethality of krz[d1]/krz[S047819]. The surviving flies are sterile and display slightly folded wings. Wing discs in rescued larvae have a normal appearance. (Molnar et al., 2011) Expression of krz[+t5.7] in krz[S047819] mutant animals fully rescues the embryonic nervous system and cuticule phenotype. (Tipping et al., 2010) 49% of krz[S047819] mutant cuticles display a large hole either at the anterior or posterior region of the embryo, 31% of mutant embryos display two holes, and 17% of the cuticles display twisting along the anterior-posterior axis. Mutants display gastrulation defects and embryonic lethality. The embryonic nervous system is disrupted in stage 15 krz[S047819] embryos, and animals display holes in the nerve cord, or a twisted nerve cord phenotype. (Tipping et al., 2010) Expression of rl[Sem.S.Scer\UAS] under the control of Scer\GAL4[da.G32] in a krz[S047819]/+ mutant background results in the development of even more additional veins in the wing and a further reduction in wing size. Expression of rl[Sem.S.Scer\UAS] and krz[Scer\UAS.T:Zzzz\GS-TAP] under the control of Scer\GAL4[da.G32] in a krz[S047819]/+ mutant background restores the wing vein phenotype to that seen in animals expressing just rl[Sem.S.Scer\UAS] under the control of Scer\GAL4[da.G32] in a wild-type background. Wing size is larger in these flies compared to animals expressing rl[Sem.S.Scer\UAS] under the control of Scer\GAL4[da.G32] in a wild-type background. The mild irregularities in ommatidial pattern observed in animals expressing rl[Sem.S.Scer\UAS] under the control of Scer\GAL4[da.G32] is strongly enhanced in a krz[S047819]/+ mutant background, and smaller rougher eyes are observed. Expression of krz[Scer\UAS.T:Zzzz\GS-TAP] restores the regular ommatidial pattern and size of the eye in animals expressing rl[Sem.S.Scer\UAS] under the control of Scer\GAL4[da.G32] in a krz[S047819]/+ mutant background. The survival rate of animals expressing rl[Sem.S.Scer\UAS] under the control of Scer\GAL4[da.G32] drops to 8% in a krz[S047819]/+ mutant background. The survival rate of animals expressing rl[Sem.S.Scer\UAS] under the control of Scer\GAL4[da.G32] in a krz[S047819]/+ mutant background increases to 38% when krz[Scer\UAS.T:Zzzz\GS-TAP] is co-expressed. (Tipping et al., 2010) Homozygous adults which have been rescued from lethality by expression of krz[Scer\UAS.cRa] under the control of Scer\GAL4[hs.PB] during development (using twice daily heat shocks) die at a faster rate than heterozygous siblings or wild-type controls on medium containing no added NaCl. On medium containing 0.4M NaCl, homozygous adults die at a much faster rate than heterozygous siblings or wild-type controls. (Johnson et al., 2008) Expression of krz[Scer\UAS.T:Zzzz\TAP] under the control of Scer\GAL4[da.G32] in a krz[S047819] mutant background completely rescues lethality. Expression of krz[Scer\UAS.T:Zzzz\GS-TAP] under the control of Scer\GAL4[da.G32] in a krz[S047819] mutant background completely rescues lethality. (Kyriakakis et al., 2008) krz^S047819 homozygous adults which have been rescued from lethality by expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^hs.PB show a reduced initial activity phase (during the first minute) in an open field arena compared to control flies. The spontaneous locomotor activity is not significantly different from control flies from the second minute onwards (the assay was carried out for 10 minutes). krz^S047819 homozygous adults which have been rescued from lethality by expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^hs.PB and which are also expressing krz^Scer\UAS.cRa under the control of Scer\GAL4^{Or83b.T:Hsim\VP16} still show a reduced initial activity phase in an open field arena compared to control flies. There are no significant differences in spontaneous activity between krz^S047819 homozygous adults which have been rescued from lethality by expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^hs.PB and control flies measured in the dark over a period of days. krz^S047819 homozygous adults which have been rescued from lethality by expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^hs.PB fail to show a robust increase in activity after mechanical stimulation, in contrast to control flies. (Liu et al., 2007) The majority of homozygous krz^S047819 mutants die as embryos, and less than 25% reach the third instar larval stage (Mukherjee et al., 2005) The notching and vein thickening phenotypes seen in dx^S1M hemizygous males are enhanced by krz^S047819/+. Survival of N^Ax-9/N^Ax-E2 dx^ENU/+ flies to adulthood is significantly decreased by krz^S047819/+. (Mukherjee et al., 2005) Homozygotes die throughout development. Most animals fail to hatch and none of the surviving larvae live to puparium formation. Prior to death the mutant larvae become immobile and flaccid. Third instar larvae develop melanotic tumours, which form throughout the body cavity but most frequently collect in the posterior of the larva. Tumours are frequently found floating free in the haemocoel. Large numbers of lamellocytes are found circulating through the haemocoel of mutant third instar larvae (these cells typically form in wild-type pupae). The fat bodies of mutant larvae shortly after the second moult have a rough appearance compared to those of wild-type larvae. At approximately 18-20 hours after the second moult the fat bodies of surviving mutant larvae begin to dissociate into individual cells (the wild-type fat body does not normally dissociate until after pupariation). Melanotic tumours begin to form on the surface of the mutant fat bodies shortly after the second moult. (Roman et al., 2000) Fully complements the male sterility of mod⁰⁷⁵⁷⁰. Lethality is rescued by krz^+t12. krz^+t5.8 rescues the lethality, formation of melanotic tumours and disaggregation of fat bodies seen in krz^S047819 animals. Expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^hs.PB using heat shock is sufficient to rescue some of the krz^S047819 mutant animals at each larval instar. Rescue of lethality becomes significantly more efficient at older stages. Expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^Kr.PM does not rescue the lethality of krz^S047819 animals. Expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^elav-C155 rescues the lethality of krz^S047819 animals. Approximately 1/3 of the rescued animals fail to expand their wings after eclosion. Melanotic tumours are visible in the abdomens of most of the rescued animals. (Roman et al., 2000)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}krz^S047819 (Roman et al., 2000, FlyBase, 1992-) P{lacW}l(3)S047819^S047819 (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 9 )
Research paper	Molnar et al., 2011, PLoS Genet. 7(3): e1001335 Role of the Drosophila non-visual ß-arrestin kurtz in hedgehog signalling. [FBrf0213301] Tipping et al., 2010, EMBO J. 29(19): 3222--3235 β-arrestin Kurtz inhibits MAPK and Toll signalling in Drosophila development. [FBrf0211995] Johnson et al., 2008, Insect Biochem. Mol. Biol. 38(11): 1016--1022 Functional characterization of kurtz, a Drosophila non-visual arrestin, reveals conservation of GPCR desensitization mechanisms. [FBrf0207367] Kyriakakis et al., 2008, Fly 2(4): 229--235 Tandem affinity purification in Drosophila: The advantages of the GS-TAP system. [FBrf0210465] Liu et al., 2007, Genetics 175(3): 1197--1212 Exploratory activity in Drosophila requires the kurtz nonvisual arrestin. [FBrf0200160] Mukherjee et al., 2005, Nat. Cell Biol. 7(12): 1191--1201 Regulation of Notch signalling by non-visual beta-arrestin. [FBrf0191265] Roman et al., 2000, Genetics 155(3): 1281--1295 kurtz, a novel nonvisual arrestin, is an essential neural gene in Drosophila. [FBrf0128623] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacW}krzS047819 Insertion

Dmel\P{lacW}krz^S047819 Insertion