FlyBase Insertion Report: Dmel\P{GawB}c316

General Information

Symbol

Dmel\P{GawB}c316

Species

D. melanogaster

Name

FlyBase ID

FBti0017056

Feature type

transposable_element_insertion_site

Also Known As

c316-GAL4, c316, c316{GAL4}, Amn(c316)-Gal4, DPM-Gal4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4

Viability / fertility

Associated allele(s)

Scer\GAL4^c316

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

(FlyBase, 1992-)

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Chen et al., 2012, Lee et al., 2011, Pitman et al., 2011, Wu et al., 2011, Donlea et al., 2009, Belgacem and Martin, 2007, Krashes et al., 2007, Mattaliano et al., 2007, Ferris et al., 2006, Keene et al., 2006, Carhan et al., 2005, Yu et al., 2005, Keene et al., 2004, Tamura et al., 2003, Waddell et al., 2000)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^c316

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

mushroom body dorsal paired medial cell

Comment: strong expression

(Wu et al., 2023, Higham et al., 2019, Chen et al., 2012, Lee et al., 2011, Pitman et al., 2011, Wu et al., 2011, Krashes et al., 2007, Waddell et al., 2000)

Kenyon cell | subset

(Wu et al., 2011)

adult brain | restricted

(Lee et al., 2011, Wu et al., 2011)

adult mushroom body alpha-lobe | restricted

(Pitman et al., 2011)

adult mushroom body beta-lobe | restricted

(Pitman et al., 2011)

mushroom body pedunculus | restricted

(Pitman et al., 2011)

neuron | subset of adult brain

(Chen et al., 2012)

adult mushroom body

Comment: medium expression

(Chen et al., 2012)

glial cell of adult brain

Comment: strong expression

(Chen et al., 2012)

eye

Comment: medium expression

(Chen et al., 2012)

adult mushroom body beta'-lobe | restricted

(Pitman et al., 2011)

adult mushroom body alpha'-lobe | restricted

(Pitman et al., 2011)

Additional Information

Statement

Reference

Drives expression in the mushroom body dorsal paired medial cell.

(FlyBase Curators, 2017)

ScerGAL4^c316 is expressed in several cells of the adult brain, including a subset of Kenyon cells and in the mushroom body dorsal paired medial neurons.

(Wu et al., 2011)

ScerGAL4^c316 is expressed in the mushroom body dorsal paired medial cell, and in that cell's dense arborization in the mushroom body alpha'/beta'-lobes. Use of GFP-reconstitution across synaptic partners (GRASP) reveals that there are close contacts between the mushroom body dorsal paired medial cell and the mushroom body neurons in the mushroom body lobes and pedunculus. The terminals of the mushroom body dorsal and anterior paired medial cells are in close association in the mushroom body alpha'/beta' lobes and in the pedunculus. Co-expression of ScerGAL4^c316 with a presynaptic marker preferentially labels the presynaptic terminals of the mushroom body dorsal paired medial cell in the mushroom body alpha/beta lobes.

(Pitman et al., 2011)

ScerGAL4^c316 is expressed in a subset of cells in the adult brain, including the mushroom body dorsal paired medial cells.

(Lee et al., 2011)

Marker for

mushroom body dorsal paired medial cell

(Wu et al., 2023, Lee et al., 2011, Pitman et al., 2011, Krashes et al., 2007)

Reflects expression of

amn

(Higham et al., 2019)

Reporter construct used in assay

Ecol\lacZ^UAS.cBa

(Waddell et al., 2000)

Mmus\Cd8a^UAS.mCherry

(Pitman et al., 2011)

brp^UAS.GFP

(Pitman et al., 2011)

Avic\GFP^{spGFP1-10.UAS.Tag:M(hCD4)}

(Pitman et al., 2011)

Mmus\Cd8a^UAS.cLa.EGFP

(Lee et al., 2011, Wu et al., 2011)

Dscam1^{exon17.2.UAS.GFP}

(Krashes et al., 2007)

shi^1.UAS

(Krashes et al., 2007)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

30830

w^*; P{GawB}c316

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

Amn(c316)-Gal4

(Cavaliere et al., 2013)

Amn-Gal4

(Cavaliere et al., 2013)

C316

(Donlea et al., 2009)

C316-GAL4

(Haynes et al., 2015, Wu et al., 2011)

C316-Gal4

(Wu et al., 2023, Weiss and Donlea, 2021, Sun et al., 2020)

DPM-Gal4

(Wu et al., 2023, Kanellopoulos et al., 2020)

P^GAL4C316

(Belgacem and Martin, 2007)

P{GAL4:c316}

(Carhan et al., 2005)

P{GAL4}c316

(Waddell et al., 2000, FlyBase, 1992-)

P{GawB}c316

amn(c316)-Gal4

(Higham et al., 2019)

c316

(Turrel et al., 2016, Pitman et al., 2011, Hong et al., 2008, Krashes et al., 2007, Ferris et al., 2006, Tamura et al., 2003)

c316 {GAL4}

(Keene et al., 2006)

c316-GAL4

(Bonanno et al., 2024, Al-Anzi and Zinn, 2018, Steck et al., 2018, Guven-Ozkan et al., 2016, Tong et al., 2016, Mattaliano et al., 2007, Yu et al., 2005)

c316-Gal4

(Draper et al., 2024, Georganta et al., 2021, Mayseless et al., 2018, Valadas et al., 2018, Chen et al., 2012, Tonoki and Davis, 2012, Lee et al., 2011, Neely et al., 2011)

c316-gal4

(Tonoki and Davis, 2015)

c316{GAL4}

(Krashes et al., 2007, Keene et al., 2004, Waddell et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (37)

Report Sections

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