Dmel\P{GT1}BG01978 Insertion

General Information
Symbol	Dmel\P{GT1}BG01978	Species	D. melanogaster
Name		FlyBase ID	FBti0017668
Feature type	transposable_element_insertion_site
Description
Inserted element	P{GT1}	Expression data
Affected gene(s)	twit	Viability / fertility	viable, fertile
Causes allele(s)		Stock availability	1 publicly available
LINE ID	BG01978
Genomic Location
Chromosomal location	2L ( 38F2 )	Sequence location	2L:20,852,538..20,852,538 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{GT1}.BG A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GT1}; created and vetted by the Gene Disruption Project (GDP). The P{GT1} construct is described as a dual-tagged gene trap (FBrf0132344):it carries (1) w[+mGT1] mini-white marker, designed to confer a pigmented eye phenotype only if the construct is inserted within a host gene, and (2) a promoter-less Ser\GAL4 gene which is expressed if it is produced as a fusion with the mRNA produced by the host gene. The construct also carries a Tn\neoR selectable marker (FBrf0132344). (Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:20,852,538..20,852,538 [+] (Gene Disruption Project members, 2001-)
Orientation
Cytological location (computed by FlyBase)	38F2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	38F1 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	BH011449 (Gene Disruption Project members, 2001-, FlyBase, 1992-)
Inserted Element
Construct	P{GT1}
Location-dependent role	GAL4 gene trap (Lukacsovich and Asztalos, 1999.7.15)
Size	8.447, 8.447Kb
Associated alleles	Scer\GAL4^GT1 Tn\neoR^Hsp70Bb.PS w^+mGT
Molecular map
Affected Gene(s)
Insertion may affect gene	twit (Gene Disruption Project members, 2001-)
Alleles and Phenotypes
Causes alleles

Lethality References viable (Gene Disruption Project members, 2001-)
Sterility References fertile (Gene Disruption Project members, 2001-)
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	BG01978 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	12591 w¹¹¹⁸; P{GT1}BG01978
Linkouts
Comments
	Location 2L:20840131-20840132 confirmed by FlyBase alignment of dbGSS accession BH011449 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	BG01978 (Gene Disruption Project members, 2001-, FlyBase, 1992-) P{GT1}BG01978 (FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 7 )
Research paper	Kim and Marqués, 2012, Dev. Neurobiol. 72(12): 1541--1558 The Ly6 neurotoxin-like molecule target of wit regulates spontaneous neurotransmitter release at the developing neuromuscular junction in Drosophila. [FBrf0219871] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Lukacsovich and Asztalos, 1999.7.15, Dual tagging gene-trap of novel genes in Drosophila melanogaster. Dual tagging gene-trap of novel genes in Drosophila melanogaster. [FBrf0108534]
FlyBase analysis	FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]