A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{GT1}eysBG02208 Insertion

General Information
Symbol Dmel\P{GT1}eysBG02208 Species D. melanogaster
Name FlyBase ID FBti0017696
Feature type transposable_element_insertion_site
Description
Inserted element P{GT1} Expression data
Affected gene(s) eys Viability / fertility viable, fertile
Causes allele(s) eysBG02208 Stock availability 1 publicly available
LINE ID BG02208
Genomic Location
Chromosomal location 2L ( 22E1 ) Sequence location 2L:2,357,287..2,357,287 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0049083 FBti0131568 FBti0045931 FBti0067091 FBti0051320 FBti0067660 FBti0054055 FBti0076930 FBti0017696 FBti0035535 FBti0047767 FBti0044825 FBti0056918 FBti0024519 FBti0026255 FBti0048059 FBti0124516 FBti0113196 FBti0021187 FBti0026184 FBti0034036 FBti0110486 FBti0057214 FBti0040122 FBti0076938 FBti0112155 FBti0006638 FBti0101196 FBti0005163 FBti0109008 FBti0066859 FBti0109966 FBti0027596 FBti0102245
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GT1}; created and vetted by the Gene Disruption Project (GDP). The P{GT1} construct is described as a dual-tagged gene trap (FBrf0132344):it carries (1) w[+mGT1] mini-white marker, designed to confer a pigmented eye phenotype only if the construct is inserted within a host gene, and (2) a promoter-less Ser\GAL4 gene which is expressed if it is produced as a fusion with the mRNA produced by the host gene. The construct also carries a Tn\neoR selectable marker (FBrf0132344).
(Bellen et al., 2004)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:2,357,287..2,357,287 [-]
(Gene Disruption Project members, 2001-)
Orientation
Cytological location
(computed by FlyBase)
22E1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
22E1 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
BH011478
(Gene Disruption Project members, 2001-, FlyBase, 1992-)
hide Inserted Element
Construct P{GT1}
Location-dependent
role
GAL4 gene trap
(Lukacsovich and Asztalos, 1999.7.15)
Size 8.447, 8.447Kb
Associated alleles
Scer\GAL4GT1
Tn\neoRHsp70Bb.PS
w+mGT
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
eys
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
eysBG02208
(Gene Disruption Project members, 2001-, Zelhof, 2006)
Lethality
References
viable
(Gene Disruption Project members, 2001-)
Sterility
References
fertile
(Gene Disruption Project members, 2001-)
hide Phenotype Manifest In
photoreceptor cell
(Husain et al., 2006)
hide Detailed Description
Statement
Reference
Inter-rhabdomeral space is prevented in eysBG02208 mutants.
(Husain et al., 2006)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
BG02208
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
12661
w1118; P{GT1}eysBG02208
hide Linkouts
hide Comments
Location 2L:2357287-2357288 confirmed by FlyBase alignment of dbGSS accession BH011478 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
BG02208
(Gene Disruption Project members, 2001-, FlyBase, 1992-, Zelhof, 2006)
P{GT1}CG7245BG02208
(FlyBase, 1992-)
P{GT1}eysBG02208
(FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 8 )
Research paper
Husain et al., 2006, Dev. Cell 11(4): 483--493
The agrin/perlecan-related protein eyes shut is essential for epithelial lumen formation in the Drosophila retina. [FBrf0193952]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Supplementary material
Zelhof, 2006, Nature 443(7112):
[FBrf0198649]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
Lukacsovich and Asztalos, 1999.7.15, Dual tagging gene-trap of novel genes in Drosophila melanogaster.
Dual tagging gene-trap of novel genes in Drosophila melanogaster. [FBrf0108534]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]