Dmel\P{GT1}robo^BG01092 Insertion

General Information
Symbol	Dmel\P{GT1}robo^BG01092	Species	D. melanogaster
Name		FlyBase ID	FBti0018081
Feature type	transposable_element_insertion_site
Description
Inserted element	P{GT1}	Expression data
Affected gene(s)	robo	Viability / fertility	viable, fertile
Causes allele(s)	robo^BG01092	Stock availability	1 publicly available
LINE ID	BG01092
Genomic Location
Chromosomal location	2R ( 59A3 )	Sequence location	2R:18,588,259..18,588,259
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:18,588,259..18,588,259 (Gene Disruption Project members, 2001-)
Orientation
Cytological location (computed by FlyBase)	59A3 ( inferred by FlyBase from sequence location )
Cytological location (reported)	58F6 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	BH023725 (Gene Disruption Project members, 2001-, FlyBase, 1992-)
Inserted Element
Construct	P{GT1}
Location-dependent role	GAL4 gene trap (Lukacsovich and Asztalos, 1999.7.15)
Size	8.447, 8.447Kb
Associated alleles	Scer\GAL4^GT1 Tn\neoR^Hsp70Bb.PS w^+mGT
Molecular map
Affected Gene(s)
Insertion may affect gene	robo (Gene Disruption Project members, 2001-, Berni et al., 2008)
Alleles and Phenotypes
Causes alleles	robo^BG01092 (Gene Disruption Project members, 2001-, Harbison et al., 2004, Norga et al., 2003, Berni et al., 2008)

Lethality References viable (Gene Disruption Project members, 2001-)
Sterility References fertile (Gene Disruption Project members, 2001-)
Phenotype Manifest In
	commissure (Berni et al., 2008) longitudinal connective (Berni et al., 2008) macrochaeta (Norga et al., 2003) midline crossing tract (Berni et al., 2008) presumptive embryonic/larval central nervous system (Berni et al., 2008)
Detailed Description
Statement Reference robo[BG01092] homozygous flies show a consistent and significant shortening of the period of the behavioural rhythm compared to wild-type controls under free-running conditions. Heterozygous robo[BG01092] mutants exhibit largely normal behavioural rhythms. robo[BG01092] mutant flies exhibit a similar avoidance response to benzaldehyde as in wild-type. robo[BG01092] mutants display normal eclosion behaviour. Homozygous robo[BG01092] mutants exhibit axon guidance defects. The overall pattern of the CNS axon scaffold is fairly normal; the longitudinal axon tracts as well as the anterior and posterior commissures are spaced normally and usually have normal thickness. The main defect observed is related to the anterior defasciculation of the longitudinal tracts, whereby in certain segments up to five axonal bundles can be observed. in addition, commissural bundles in some segments appear slightly abnormal and wander between commissures, extending their projections along and over the midline instead of projecting towards the contralateral side, resulting in a distinct phenotype. Quantification reveals that the most prominent defect in robo[BG01092] individuals is the defasciculation of longitudinal axon bundles. robo[BG01092] responds to a light pulse during the early night (ZT 15) with an approximate 3hr delay, as in wild-type, although the phase shift in mutant flies is larger than in wild-type. After ZT 21, the magnitude of the hase respose is slightly smaller in robo[BG01092] individuals compared to wild-type. After 10 days under synchronizing temperature cycles (25-20[o]C, where the warmer temperatures are reminiscent of the light period), where robo[BG01092] flies disply close to 24hr rhythms under temperature entrainment, as wild-type, the endogenous free-running period is significantly shorter in robo[BG01092] homozygotes than in wild-type controls robo[BG01092] mutants display a similar morning anticipation as wild-type in a 12hr LD regime. However the anticipation of the evening transition starts at least half an hour earlier than in wild-type controls, consistent with the shorter periodicity in the activity rhythms in free-running conditions. Small LNv neurons are mislocalised in roughly half of the robo[BG01092] brain hemispheres analysed. Misplaced neurons are located in the dorsal protocerebrum and occasionally found next to the large LNvs. Supernumerary cells are not detected in pdf-positive clusters robo[BG01092] brains. Subtly altered axon trajectories are found in a high proportion of the dissected robo[BG01092] brains. The axon bundle running along the posterio optic tract does not run in the same way in robo[BG01092] brains as in wild-type. To quantify this difference the angle between the initial turn of the axon fibre and a base line with the same origin that reaches to an intermediate point in the posterior optic tract just on top of the esophageal foramen at the midline. Using this assay, wild-type brains tend to display \'negative\' angles, while most robo[BG01092] brains exhibit \'positive\' angles. robo[BG01092] brains show no apparent difference in the trajectory of photoreceptor axons at the level of the optic lobe. robo[BG01092] is homozygous viable. robo[BG01092]/robo[1] transheterozygotes exhibit ectopic midline crossing. (Berni et al., 2008) robo[BG01092] Pdf[01] double mutants exhibit a lengthening of the free-running period in a dose-dependent manner. Introducing a copy of robo[BG01092] into Pdf[01] mutants gives rise to >80% rhythmic individuals. The average activity plot of robo[BG01092] Pdf[01] flies shows a modest increase in the activity ~1hr before the lights-on transition, indicating that the morning anticipation is rescued even in the absence of Pdf neuropeptide. robo[BG01092] Pdf[01] double mutants show a significantly lengthened period of locomotor activity. Transheterozygous robo[BG01092] Pdf[01] flies display wild-type period and rhythmicity. (Berni et al., 2008) Homozygous males and females show a significant increase in starvation resistance compared to controls. (Harbison et al., 2004) Mutants exhibit a quantitative gain of bristles. (Norga et al., 2003)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	BG01092 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	12778 w¹¹¹⁸; P{GT1}robo^BG01092
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{GT1}robo^BG01092 (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 7 )
Research paper	Berni et al., 2008, Europ. J. Neurosci. 27(2): 396--407 The axon-guidance roundabout gene alters the pace of the Drosophila circadian clock. [FBrf0202846] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Harbison et al., 2004, Genetics 166(4): 1807--1823 Quantitative trait loci affecting starvation resistance in Drosophila melanogaster. [FBrf0174696] Norga et al., 2003, Curr. Biol. 13(16): 1388--1397 Quantitative analysis of bristle number in Drosophila mutants identifies genes involved in neural development. [FBrf0162000]
Personal communication to FlyBase	Lukacsovich and Asztalos, 1999.7.15, Dual tagging gene-trap of novel genes in Drosophila melanogaster. Dual tagging gene-trap of novel genes in Drosophila melanogaster. [FBrf0108534]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177]

Dmel\P{GT1}roboBG01092 Insertion

Dmel\P{GT1}robo^BG01092 Insertion