Dmel\P{GT1}Pka-C1^BG02142 Insertion

General Information
Symbol	Dmel\P{GT1}Pka-C1^BG02142	Species	D. melanogaster
Name		FlyBase ID	FBti0018116
Feature type	transposable_element_insertion_site
Description
Inserted element	P{GT1}	Expression data
Affected gene(s)	Pka-C1, Scer\GAL4	Viability / fertility
Causes allele(s)	Pka-C1^BG02142, Scer\GAL4^{Pka-C1-BG02142}	Stock availability	3 publicly available
LINE ID	BG02142
Genomic Location
Chromosomal location	2L ( 30C5 )	Sequence location	2L:9,699,218..9,699,218 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{GT1}.BG A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GT1}; created and vetted by the Gene Disruption Project (GDP). The P{GT1} construct is described as a dual-tagged gene trap (FBrf0132344):it carries (1) w[+mGT1] mini-white marker, designed to confer a pigmented eye phenotype only if the construct is inserted within a host gene, and (2) a promoter-less Ser\GAL4 gene which is expressed if it is produced as a fusion with the mRNA produced by the host gene. The construct also carries a Tn\neoR selectable marker (FBrf0132344). (Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:9,699,218..9,699,218 [+] (Gene Disruption Project members, 2001-)
Orientation
Cytological location (computed by FlyBase)	30C5 ( inferred by FlyBase from sequence location )
Cytological location (reported)	30C6 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	BH023761 (Gene Disruption Project members, 2001-, FlyBase, 1992-)
Inserted Element
Construct	P{GT1}
Location-dependent role	GAL4 gene trap (Lukacsovich and Asztalos, 1999.7.15)
Size	8.447, 8.447Kb
Associated alleles	Scer\GAL4^GT1 Tn\neoR^Hsp70Bb.PS w^+mGT
Molecular map
Affected Gene(s)
Insertion may affect gene	Pka-C1 (Gene Disruption Project members, 2001-, Chen and Ganetzky, 2012, Tunstall et al., 2012) Scer\GAL4 (Tunstall et al., 2012)
Alleles and Phenotypes
Causes alleles	Pka-C1^BG02142 (Gene Disruption Project members, 2001-, Dworkin and Gibson, 2006, Chen and Ganetzky, 2012, Tunstall et al., 2012) Scer\GAL4^{Pka-C1-BG02142} (Tunstall et al., 2012)

Lethality References lethal (Tunstall et al., 2012) lethal \| recessive (Dworkin and Gibson, 2006)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference Heterozygosity for both Pka-C1[BG02142] and Df(1)Exel9051 results in a significant reduction in neuromuscular junction (NMJ) synaptic bouton numbers, compared with either single mutant or wild-type. (Chen and Ganetzky, 2012) Pka-C1[BG02142] is homozygous lethal. (Tunstall et al., 2012)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	BG02142 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 3 )
Bloomington	12752 w¹¹¹⁸; P{GT1}Pka-C1^BG02142/CyO
Kyoto	108885 w¹¹¹⁸; P{GT1}Pka-C1^BG02142/CyO 111486 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{GT1}Pka-C1^BG02142 P{neoFRT}40A/CyO y⁺
Linkouts
Comments
	Location 2L:9699218-9699219 confirmed by FlyBase alignment of dbGSS accession BH023761 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	BG02142 (Gene Disruption Project members, 2001-, FlyBase, 1992-, Tunstall et al., 2012) P{GT1}Pka-C1^BG02142 (FlyBase, 2005, FlyBase, 1992-, ) PKA-C1^BG02142 (Chen and Ganetzky, 2012) PKA-C1^GT (Chen and Ganetzky, 2012)
Secondary FlyBase IDs

References ( 9 )
Research paper	Chen and Ganetzky, 2012, J. Cell Biol. 196(4): 529--543 A neuropeptide signaling pathway regulates synaptic growth in Drosophila. [FBrf0217500] Tunstall et al., 2012, PLoS ONE 7(4): e35641 A Screen for Genes Expressed in the Olfactory Organs of Drosophila melanogaster Identifies Genes Involved in Olfactory Behaviour. [FBrf0218110] Dworkin and Gibson, 2006, Genetics 173(3): 1417--1431 Epidermal growth factor receptor and transforming growth factor-beta signaling contributes to variation for wing shape in Drosophila melanogaster. [FBrf0193945] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Lukacsovich and Asztalos, 1999.7.15, Dual tagging gene-trap of novel genes in Drosophila melanogaster. Dual tagging gene-trap of novel genes in Drosophila melanogaster. [FBrf0108534]
FlyBase analysis	FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{GT1}Pka-C1BG02142 Insertion

Dmel\P{GT1}Pka-C1^BG02142 Insertion