A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{GT1}Sac1BG02228 Insertion

General Information
Symbol Dmel\P{GT1}Sac1BG02228 Species D. melanogaster
Name FlyBase ID FBti0018121
Feature type transposable_element_insertion_site
Description
Inserted element P{GT1} Expression data
Affected gene(s) Sac1 Viability / fertility
Causes allele(s) Sac1BG02228 Stock availability 2 publicly available
LINE ID BG02228
Genomic Location
Chromosomal location 3L ( 61F4 ) Sequence location 3L:1,248,056..1,248,056 [-]
Map ( GBrowse ) detailed view FBti0050885 FBti0102159 FBti0005583 FBti0002901_2 FBti0002901_1 FBti0038866 FBti0009923 FBti0105118 FBti0056457 FBti0104317 FBti0125469_2 FBti0125469_1 FBti0112782 FBti0113175 FBti0039663 FBti0105797 FBti0105806 FBti0018121 FBti0106257 FBti0043437 FBti0125470_2 FBti0125470_1 FBti0132694 FBti0113946
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:1,248,056..1,248,056 [-]
(Gene Disruption Project members, 2001-)
Orientation
Cytological location
(computed by FlyBase)
61F4 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
61F4 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
BH023767
(Gene Disruption Project members, 2001-, FlyBase, 1992-)
hide Inserted Element
Construct P{GT1}
Location-dependent
role
GAL4 gene trap
(Lukacsovich and Asztalos, 1999.7.15)
Size 8.447, 8.447Kb
Associated alleles
Scer\GAL4GT1
Tn\neoRHsp70Bb.PS
w+mGT
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Sac1
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
Sac1BG02228
(Gene Disruption Project members, 2001-, Wei et al., 2003)
Lethality
References
lethal | embryonic stage | recessive
(Wei et al., 2003)
Sterility
References
hide Phenotype Manifest In
embryonic/first instar larval cuticle | dorsal
(Wei et al., 2003)
hide Detailed Description
Statement
Reference
pucE69 Sac1L2F double heterozygous embryos show defects in the dorsal cuticle (anterior head holes and/or puckering). Sac1BG02228 shows no genetic interactions with pucE69 or bsk1.
(Wei et al., 2003)
Homozygous embryos have dorsal anterior holes in the cuticle. The mutant embryos do not show premature apical constriction of amnioserosa cells. Sac1BG02228/Sac1L2F embryos have dorsal anterior holes in the cuticle and puckering of the dorsal cuticle.
(Wei et al., 2003)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
BG02228
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
12780
w1118; P{GT1}Sac1BG02228/TM6B, Tb1
Kyoto
114332
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{GT1}Sac1BG02228 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb1
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hide Comments
Location 3L:1231588-1231589 confirmed by FlyBase alignment of dbGSS accession BH023767 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{GT1}CG9128BG02228
(FlyBase, 1992-)
P{GT1}Sac1BG02228
(FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 6 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Wei et al., 2003, Curr. Biol. 13(21): 1882--1887
The Sac1 lipid phosphatase regulates cell shape change and the JNK cascade during dorsal closure in Drosophila. [FBrf0167407]
Personal communication to FlyBase
Lukacsovich and Asztalos, 1999.7.15, Dual tagging gene-trap of novel genes in Drosophila melanogaster.
Dual tagging gene-trap of novel genes in Drosophila melanogaster. [FBrf0108534]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]