A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{GT1}Hmt4-20BG00814 Insertion

General Information
Symbol Dmel\P{GT1}Hmt4-20BG00814 Species D. melanogaster
Name FlyBase ID FBti0018384
Feature type transposable_element_insertion_site
Description
Inserted element P{GT1} Expression data
Affected gene(s) Hmt4-20 Viability / fertility viable, fertile
Causes allele(s) Hmt4-20BG00814 Stock availability 1 publicly available
LINE ID BG00814
Genomic Location
Chromosomal location X ( 1B13 ) Sequence location X:543,525..543,525 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0063323 FBti0040187 FBti0114780 FBti0018384 FBti0011838 FBti0151491 FBti0049798 FBti0039449 FBti0055535 FBti0023358 FBti0034590 FBti0029703 FBti0012828 FBti0015685 FBti0071585 FBti0012840 FBti0029208 FBti0007441 FBti0071247 FBti0052408 FBti0049038 FBti0023210
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GT1}; created and vetted by the Gene Disruption Project (GDP). The P{GT1} construct is described as a dual-tagged gene trap (FBrf0132344):it carries (1) w[+mGT1] mini-white marker, designed to confer a pigmented eye phenotype only if the construct is inserted within a host gene, and (2) a promoter-less Ser\GAL4 gene which is expressed if it is produced as a fusion with the mRNA produced by the host gene. The construct also carries a Tn\neoR selectable marker (FBrf0132344).
(Bellen et al., 2004)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
X:543,525..543,525 [+]
(Gene Disruption Project members, 2001-)
Orientation
Cytological location
(computed by FlyBase)
1B13 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
1B10 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
AZ933374
(Gene Disruption Project members, 2001-, FlyBase, 1992-)
hide Inserted Element
Construct P{GT1}
Location-dependent
role
GAL4 gene trap
(Lukacsovich and Asztalos, 1999.7.15)
Size 8.447, 8.447Kb
Associated alleles
Scer\GAL4GT1
Tn\neoRHsp70Bb.PS
w+mGT
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Hmt4-20
(Gene Disruption Project members, 2001-, Phalke et al., 2009, Greil et al., 2007, Sakaguchi et al., 2008)
hide Alleles and Phenotypes
Causes alleles
Hmt4-20BG00814
(Schotta et al., 2004, Phalke et al., 2009, Greil et al., 2007, Sakaguchi et al., 2008)
Lethality
References
viable
(Gene Disruption Project members, 2001-, Schotta et al., 2004)
Sterility
References
fertile
(Gene Disruption Project members, 2001-)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Hmt4-20[BG00814] does not dominantly affect position effect variegation at the Sb locus which is caused by T(2;3)Sb[V].
(Sakaguchi et al., 2008)
Hmt4-20[BG00814] suppresses the position effect variegation of the mutant Sb phenotype which is seen in the T(2;3)Sb[V] chromosome.
(Sasai et al., 2007)
Suv4-20BG00814 homozygotes suppress the variegation of Sb caused by T(2;3)SbV, resulting in an increase in the number of adults with a Sb mutant phenotype (short bristles).
(Schotta et al., 2004)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
BG00814
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
12510
P{GT1}Hmt4-20BG00814 w1118
hide Linkouts
hide Comments
Location X:506313-506314 confirmed by FlyBase alignment of dbGSS accession AZ933374 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
BG00814
(Gene Disruption Project members, 2001-, FlyBase, 1992-, Phalke et al., 2009)
P{GT1}BG00814
(FlyBase, 1992-)
P{GT1}Hmt4-20BG00814
 
P{GT1}Suv4-20BG00814
(FlyBase, 2005, FlyBase, 1992-, Greil et al., 2007)
PBG00814
(Sakaguchi et al., 2008)
Secondary FlyBase IDs
hide References ( 11 )
Research paper
Phalke et al., 2009, Nat. Genet. 41(6): 696--702
Retrotransposon silencing and telomere integrity in somatic cells of Drosophila depends on the cytosine-5 methyltransferase DNMT2. [FBrf0208049]
Sakaguchi et al., 2008, Genetics 179(1): 317--322
Functional characterization of the Drosophila Hmt4-20/Suv4-20 histone methyltransferase. [FBrf0204822]
Greil et al., 2007, EMBO J. 26(3): 741--751
HP1 controls genomic targeting of four novel heterochromatin proteins in Drosophila. [FBrf0192671]
Sasai et al., 2007, FEBS J. 274(23): 6139--6151
The Drosophila jumonji gene encodes a JmjC-containing nuclear protein that is required for metamorphosis. [FBrf0202834]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Schotta et al., 2004, Genes Dev. 18(11): 1251--1262
A silencing pathway to induce H3-K9 and H4-K20 trimethylation at constitutive heterochromatin. [FBrf0178951]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
Lukacsovich and Asztalos, 1999.7.15, Dual tagging gene-trap of novel genes in Drosophila melanogaster.
Dual tagging gene-trap of novel genes in Drosophila melanogaster. [FBrf0108534]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]