A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}E23EP2297 Insertion

General Information
Symbol Dmel\P{EP}E23EP2297 Species D. melanogaster
Name FlyBase ID FBti0021100
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) E23 Viability / fertility
Causes allele(s) E23EP2297 Stock availability none publicly available
LINE ID EP(2)2297
Genomic Location
Chromosomal location 2L ( 23E5 ) Sequence location 2L:3,354,863..3,354,863 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0116173_1 FBti0056348 FBti0113567 FBti0052011 FBti0024182 FBti0054800 FBti0021100 FBti0111898 FBti0102631 FBti0057320 FBti0068799 FBti0021608 FBti0101945
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:3,354,863..3,354,863 [+]
(FlyBase, 2005)
Orientation
Cytological location
(computed by FlyBase)
23E5 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
54C1-54C1 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
Comments concerning
location
hide Sequence Data
Flanking sequence
AZ300942
(FlyBase, 1992-)
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
E23
(BDGP Project Members, 2000-, FlyBase Curators, 2008)
hide Alleles and Phenotypes
Causes alleles
E23EP2297
(FlyBase Curators, 2008)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(2)2297
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
Location 2L:3354862-3354863 determined by FlyBase alignment of dbGSS accession AZ300942 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EP(2)2297
(BDGP Project Members, 2000-, FlyBase, 1992-)
P{EP}E23EP2297
 
P{EP}EP2297
(FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 11 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Supplementary material
Parks et al., 2004, Nature Genetics 36(3):
Supplementary Table 1: Deletions generated at Bloomington between trans-heterozygous P element insertions. [FBrf0179070]
Personal communication to FlyBase
Deal and Cook, 2003.1.30, Isolation and characterization of Df(2L)BSC28.
Isolation and characterization of Df(2L)BSC28. [FBrf0155398]
Deal and Cook, 2003.4.2, Isolation and characterization of Df(2L)BSC31.
Isolation and characterization of Df(2L)BSC31. [FBrf0157306]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations.
Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]