A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}EP3632 Insertion

General Information
Symbol Dmel\P{EP}EP3632 Species D. melanogaster
Name FlyBase ID FBti0021145
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) Dsk Viability / fertility
Causes allele(s) DskEP3632 Stock availability 1 publicly available
LINE ID EP(3)3632
Genomic Location
Chromosomal location 3R ( 81F6 ) Sequence location 3R:16,536..16,536 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0061077 FBti0061078 FBti0061079 FBti0061080 FBti0061676 FBti0061786 FBti0062734 FBti0020294 FBti0061797 FBti0061757 FBti0061677 FBti0061678 FBti0061679 FBti0061680 FBti0061682 FBti0061685 FBti0061784 FBti0070648 FBti0043519 FBti0067652 FBti0050737 FBti0021145 FBti0038926 FBti0030127
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:16,536..16,536 [+]
(FlyBase, 2005)
Orientation
Cytological location
(computed by FlyBase)
81F6 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
87F7-87F7 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
Comments concerning
location
hide Sequence Data
Flanking sequence
AZ300908
(FlyBase, 1992-)
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Dsk
(BDGP Project Members, 2000-, Chen and Ganetzky, 2012)
hide Alleles and Phenotypes
Causes alleles
DskEP3632
(Chen and Ganetzky, 2012)
Lethality
References
Sterility
References
hide Phenotype Manifest In
embryonic/larval neuromuscular junction | third instar larval stage
(Chen and Ganetzky, 2012)
NMJ bouton | third instar larval stage
(Chen and Ganetzky, 2012)
hide Detailed Description
Statement
Reference
Compared with wild-type, homozygous Dsk[EP3632] mutants exhibit mild neuromuscular junction undergrowth. Compared with wild-type, Dsk[GD6123]/Dsk[EP3632] mutants exhibit mild neuromuscular junction undergrowth.
(Chen and Ganetzky, 2012)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(3)3632
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
17146
w1118; P{EP}EP3632/TM6B, Tb1
hide Linkouts
hide Comments
Location 3R:16535-16536 determined by FlyBase alignment of dbGSS accession AZ300908 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
dskEP3632
(Chen and Ganetzky, 2012)
dskEP
(Chen and Ganetzky, 2012)
EP(3)3632
(BDGP Project Members, 2000-, FlyBase, 1992-)
P{EP}EP3632
(FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 8 )
Research paper
Chen and Ganetzky, 2012, J. Cell Biol. 196(4): 529--543
A neuropeptide signaling pathway regulates synaptic growth in Drosophila. [FBrf0217500]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]