A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{SUPor-P}LpinKG00562 Insertion

General Information
Symbol Dmel\P{SUPor-P}LpinKG00562 Species D. melanogaster
Name FlyBase ID FBti0021332
Feature type transposable_element_insertion_site
Description
Inserted element P{SUPor-P} Expression data
Affected gene(s) kermit, Lpin, w Viability / fertility viable, fertile
Causes allele(s) kermitKG00562, LpinKG00562, wLpinK.KG00562 Stock availability 1 publicly available
LINE ID KG00562
Genomic Location
Chromosomal location 2R ( 44B5 ) Sequence location 2R:4,033,487..4,033,487 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0132689 FBti0066593 FBti0110188 FBti0125030 FBti0046584 FBti0069195 FBti0109399 FBti0021332 FBti0112313 FBti0106813 FBti0055342 FBti0104751 FBti0011125 FBti0105518 FBti0113004 FBti0057082 FBti0040152 FBti0066747 FBti0143321 FBti0056124 FBti0110911 FBti0026347 FBti0005206 FBti0007609 FBti0105058 FBti0111068 FBti0107164 FBti0057567 FBti0033624 FBti0125031_2 FBti0125031_1 FBti0108198 FBti0111605
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{SUPor-P}; created and vetted by the Gene Disruption Project (GDP). The P{SUPor-P} construct carries two visible markers: a w[+mC] mini-white marker flanked by su(Hw)-binding sites, designed to reduce the variablity of the phenotype of the white gene marker due position effects, in addition to the mini-yellow marker y[+mDint2].
(Bellen et al., 2004)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:4,033,487..4,033,487 [+]
(Gene Disruption Project members, 2001-)
Orientation
Cytological location
(computed by FlyBase)
44B5 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
44A8 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
BH615041
(Gene Disruption Project members, 2001-, FlyBase, 1992-)
hide Inserted Element
Construct P{SUPor-P}
Location-dependent
role
Size 11.467Kb
Associated alleles
gypsy\su(Hw)BR430
wBR.E.BR
y+mDint2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
kermit
(Gene Disruption Project members, 2001-, Pereira et al., 2011)
Lpin
(Pereira et al., 2011)
w
(Pereira et al., 2011)
hide Alleles and Phenotypes
Causes alleles
kermitKG00562
(Pereira et al., 2011)
LpinKG00562
(Pereira et al., 2011)
wLpinK.KG00562
(Pereira et al., 2011)
Lethality
References
partially lethal - majority die | dominant
(Pereira et al., 2011)
viable
(Gene Disruption Project members, 2001-)
Sterility
References
fertile
(Gene Disruption Project members, 2001-)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Homozygotes show semi-lethality (48 +/- 2%). Heterozygotes (from crosses to either Canton-S or w[1118] flies) show semi-lethality (60 +/- 7% and 52 +/- 8% respectively). The lethality of crosses between the P{SUPor-P}Lpin[KG00562] line and the Df(2R)Exel7095 deficiency which covers the mutated region (16 +- 3%) does not differ from that of wild type (17 +/- 4%), indicating that the lethality of the P{SUPor-P}Lpin[KG00562] line is a gain of function phenotype (this phenotype is associated with the overexpression of kermit in this line).
(Pereira et al., 2011)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
KG00562
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
13293
y1 w67c23; P{SUPor-P}LpinKG00562
hide Linkouts
hide Comments
Location 2R:3657795-3657796 confirmed by FlyBase alignment of dbGSS accession BH615041 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
KG00562
(Gene Disruption Project members, 2001-, FlyBase, 1992-, Pereira et al., 2011)
P{SUPor-P}KG00562
(FlyBase, 2005, FlyBase, 1992-)
P{SUPor-P}LpinKG00562
 
Secondary FlyBase IDs
hide References ( 6 )
Research paper
Pereira et al., 2011, Braz. J. med. biol. Res. 44(4): 283--290
Overexpression of kermit/dGIPC is associated with lethality in Drosophila melanogaster. [FBrf0213415]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]