A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{SUPor-P}UvragKG04163 Insertion

General Information
Symbol Dmel\P{SUPor-P}UvragKG04163 Species D. melanogaster
Name FlyBase ID FBti0021400
Feature type transposable_element_insertion_site
Description
Inserted element P{SUPor-P} Expression data
Affected gene(s) Uvrag Viability / fertility viable, fertile
Causes allele(s) UvragKG04163 Stock availability 1 publicly available
LINE ID KG04163
Genomic Location
Chromosomal location 2L ( 34B4 ) Sequence location 2L:13,286,886..13,286,886 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0131756 FBti0050411 FBti0025058 FBti0104688 FBti0107107 FBti0021400 FBti0124771 FBti0068202 FBti0049112 FBti0049610 FBti0068642 FBti0045775 FBti0077960 FBti0054279 FBti0023341 FBti0024465 FBti0124772_2 FBti0124772_1 FBti0054918
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{SUPor-P}; created and vetted by the Gene Disruption Project (GDP). The P{SUPor-P} construct carries two visible markers: a w[+mC] mini-white marker flanked by su(Hw)-binding sites, designed to reduce the variablity of the phenotype of the white gene marker due position effects, in addition to the mini-yellow marker y[+mDint2].
(Bellen et al., 2004)
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Description
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What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:13,286,886..13,286,886 [+]
(Gene Disruption Project members, 2001-)
Orientation
Cytological location
(computed by FlyBase)
34B4 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
34B3 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
BH615119
(Gene Disruption Project members, 2001-, FlyBase, 1992-)
hide Inserted Element
Construct P{SUPor-P}
Location-dependent
role
Size 11.467Kb
Associated alleles
gypsy\su(Hw)BR430
wBR.E.BR
y+mDint2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Uvrag
(Gene Disruption Project members, 2001-, Lee et al., 2011)
hide Alleles and Phenotypes
Causes alleles
UvragKG04163
(Gene Disruption Project members, 2001-, Lee et al., 2011)
Lethality
References
viable
(Gene Disruption Project members, 2001-, Lee et al., 2011)
Sterility
References
fertile
(Gene Disruption Project members, 2001-)
hide Phenotype Manifest In
male genitalia
(Lee et al., 2011)
hide Detailed Description
Statement
Reference
~50% of homozygous males show incomplete genitalia rotation, though the direction of rotation is wild type. The spermiduct does not coil around the gut in these mutants, as it does in wild type. ~75% of Uvrag[KG04163]/Uvrag[B7] or Uvrag[KG04163]/Uvrag[B21] males show incomplete genitalia rotation, though the direction of rotation is wild type.
(Lee et al., 2011)
The presence of Scer\GAL4[Abd-B-LDN] enhances the Uvrag[KG04163]/Uvrag[B21] male genital rotation phenotype, though the reason for this is unknown. Expression of N[dsRNA.P.Scer\UAS] or mam[N.Scer\UAS] using Scer\GAL4[Abd-B-LDN] partially rescues the genital rotation defect in Uvrag[KG04163]/Uvrag[B21] males.
(Lee et al., 2011)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
KG04163
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
13360
y1 w67c23; P{SUPor-P}UvragKG04163
hide Linkouts
hide Comments
Location 2L:13286886-13286887 confirmed by FlyBase alignment of dbGSS accession BH615119 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
KG04163
(Gene Disruption Project members, 2001-, FlyBase, 1992-, Lee et al., 2011)
P{SUPor-P}CG6116KG04163
(FlyBase, 2005, FlyBase, 1992-)
P{SUPor-P}UvragKG04163
 
Secondary FlyBase IDs
hide References ( 6 )
Research paper
Lee et al., 2011, Dev. Biol. 356(2): 588--597
UVRAG is required for organ rotation by regulating Notch endocytosis in Drosophila. [FBrf0214470]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]