Dmel\P{SUPor-P}Lsd-2^KG00149 Insertion

General Information
Symbol	Dmel\P{SUPor-P}Lsd-2^KG00149	Species	D. melanogaster
Name		FlyBase ID	FBti0021423
Feature type	transposable_element_insertion_site
Description
Inserted element	P{SUPor-P}	Expression data
Affected gene(s)	Lsd-2	Viability / fertility	viable, fertile
Causes allele(s)	Lsd-2^KG00149	Stock availability	1 publicly available
LINE ID	KG00149
Genomic Location
Chromosomal location	X ( 13A9 )	Sequence location	X:14,970,008..14,970,008 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	X:14,970,008..14,970,008 [+] (Gene Disruption Project members, 2001-)
Orientation
Cytological location (computed by FlyBase)	13A9 ( inferred by FlyBase from sequence location )
Cytological location (reported)	13A11 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	BH609997 (Gene Disruption Project members, 2001-, FlyBase, 1992-)
Inserted Element
Construct	P{SUPor-P}
Location-dependent role
Size	11.467Kb
Associated alleles	gypsy\su(Hw)BR⁴³⁰ w^BR.E.BR y^+mDint2
Molecular map
Affected Gene(s)
Insertion may affect gene	Lsd-2 (Gene Disruption Project members, 2001-, Gronke et al., 2003, Zhang et al., 2011)
Alleles and Phenotypes
Causes alleles	Lsd-2^KG00149 (Gene Disruption Project members, 2001-, Welte et al., 2005, Teixeira et al., 2003, Gronke et al., 2003, Gronke, 2003, Zhang et al., 2011)

Lethality References viable (Gene Disruption Project members, 2001-, Welte et al., 2005)
Sterility References fertile (Gene Disruption Project members, 2001-, Welte et al., 2005)
Phenotype Manifest In
	fat body (Gronke et al., 2003) lipid droplet \| embryonic stage \| maternal effect (Welte et al., 2005) nurse cell (Teixeira et al., 2003)
Detailed Description
Statement Reference Lsd-2[KG00149] mutants have no ectopic lipid storage in salivary glands. (Tian et al., 2011) Fat body lipid storage in Seipin[null]; Lsd-2[KG00149] animals is similar to Seipin[null] single mutants. (Tian et al., 2011) The net plus-end (phase II) transport of lipid droplets along microtubules is disrupted in Lsd-2^KG00149 embryos; lipid droplets are distributed throughout the periphery in the mutants during this phase, while droplets are localized basally in wild-type embryos. Quantification of droplet motion shows that both plus-end and minus-end periods of uninterrupted motion are slightly decreased in relation to the wild type in phase II, but the decrease in plus-end motion is larger, resulting in abolishment of net transport. For both directions, motion is slightly slower in the mutants than in wild-type embryos. The parameters of droplet motion in phase II and phase III (net minus-end transport) are essentially the same in Lsd-2^KG00149 embryos. Lsd-2^KG00149/+ embryos only show this phenotype when the Lsd-2^KG00149 allele is inherited from the mother. Embryonic development does not appear to be affected in Lsd-2^KG00149 mutants and the distribution of other organelles is not affected. Many embryos hatch and give rise to fertile adults. (Welte et al., 2005) Mutants are lean, containing significantly lower levels of triacylglycerols (37.2% of controls). (Gronke et al., 2003) Lsd-2¹/Lsd-2^KG00149 nurse cells show an abnormal pattern of neutral lipid accumulation. (Teixeira et al., 2003)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	KG00149 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	13382 y¹ P{SUPor-P}Lsd-2^KG00149; ry⁵⁰⁶
Linkouts
Comments
	Location X:14909912-14909913 confirmed by FlyBase alignment of dbGSS accession BH609997 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	KG00149 (Gene Disruption Project members, 2001-, FlyBase, 1992-) Lsd2^KG00149 (Zhang et al., 2011) P{SUPor-P}CG9057^KG00149 (FlyBase, 1992-) P{SUPor-P}Lsd-2^KG00149 (Gronke et al., 2003, FlyBase, 2005, FlyBase, 1992-) P{SUPor-P}Lsd2^KG00149 (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0027412
References ( 10 )
Research paper	Tian et al., 2011, PLoS Genet. 7(4): e1001364 Tissue-autonomous function of Drosophila seipin in preventing ectopic lipid droplet formation. [FBrf0213596] Zhang et al., 2011, Dev. Biol. 353(2): 259--265 Drosophila long-chain acyl-CoA synthetase acts like a gap gene in embryonic segmentation. [FBrf0213522] Welte et al., 2005, Curr. Biol. 15(14): 1266--1275 Regulation of lipid-droplet transport by the perilipin homolog LSD2. [FBrf0187339] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Gronke et al., 2003, Curr. Biol. 13(7): 603--606 Control of fat storage by a Drosophila PAT domain protein. [FBrf0158816] Teixeira et al., 2003, Mech. Dev. 120(9): 1071--1081 Drosophila perilipin/ADRP homologue Lsd2 regulates lipid metabolism. [FBrf0173134]
Supplementary material	Gronke, 2003, Curr. Biol. 13(7): Control of fat storage by a Drosophila PAT domain protein. [FBrf0159692]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177]

Dmel\P{SUPor-P}Lsd-2KG00149 Insertion

Dmel\P{SUPor-P}Lsd-2^KG00149 Insertion