Dmel\P{GawB}OK6 Insertion

General Information
Symbol	Dmel\P{GawB}OK6	Species	D. melanogaster
Name		FlyBase ID	FBti0023258
Feature type	transposable_element_insertion_site
Description
Inserted element	P{GawB}	Expression data	GAL4 reporter/driver
Affected gene(s)	RapGAP1, Scer\GAL4	Viability / fertility
Causes allele(s)	Scer\GAL4^Rapgap1-OK6	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	2L ( 28A4-28B2 )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	28A4-28B2 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{GawB}
Location-dependent role	GAL4 driver/enhancer trap (Brand and Perrimon, 1993)
Size	11.279Kb
Associated alleles	Scer\GAL4^wB w^+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene	RapGAP1 (Sanyal, 2009) Scer\GAL4 (Babcock et al., 2004, Franco et al., 2004, Banerjee et al., 2004, Bogdanik et al., 2004, Caygill and Johnston, 2008, Owald et al., 2010, Russo et al., 2009, Cerrato et al., 2006, Marques et al., 2002, McCabe et al., 2003, Sanchez-Soriano et al., 2005, Aberle et al., 2002, Landgraf et al., 2003, McCabe et al., 2004, Kuppers-Munther et al., 2004, Marques et al., 2003, Hekmat-Scafe et al., 2005, Sherwood et al., 2004, Ataman, 2006, Ang et al., 2006, Kittel et al., 2006, Parker et al., 2006, Serpe and O'Connor, 2006, van der Plas et al., 2006, Wang et al., 2007, Pak et al., 2011, Wang et al., 2011, Inada et al., 2011, Hallermann et al., 2010, Chen and Ganetzky, 2012, Keegan et al., 2011, Tamura et al., 2011, Wairkar et al., 2009, Cauchi et al., 2008, Sanyal, 2009, Ratnaparkhi et al., 2008, Franciscovich et al., 2008, Wu et al., 2008, Wu et al., 2008, Fradkin et al., 2008, Higashi-Kovtun et al., 2010, Banovic et al., 2010, Bachmann et al., 2010, Xiong et al., 2010, Tsurudome et al., 2010, Liu et al., 2010, Pilgram et al., 2011, Merino et al., 2009, Freeman et al., 2011, Liu et al., 2011, Szul et al., 2011, James and Broihier, 2011, Ball et al., 2010, Beck et al., 2012, Nechipurenko and Broihier, 2012, Jansen et al., 2009, Akerboom et al., 2012, Chouhan et al., 2010)
Alleles and Phenotypes
Causes alleles	Scer\GAL4^Rapgap1-OK6 (Babcock et al., 2004, Franco et al., 2004, Banerjee et al., 2004, Bogdanik et al., 2004, Marques et al., 2002, McCabe et al., 2003, Sanchez-Soriano et al., 2005, Aberle et al., 2002, Landgraf et al., 2003, McCabe et al., 2004, Kuppers-Munther et al., 2004, Caygill and Johnston, 2008, Marques et al., 2003, Owald et al., 2010, Hekmat-Scafe et al., 2005, Sherwood et al., 2004, Ataman, 2006, Ang et al., 2006, Kittel et al., 2006, Russo et al., 2009, Cerrato et al., 2006, Parker et al., 2006, Serpe and O'Connor, 2006, van der Plas et al., 2006, Wang et al., 2007, Pak et al., 2011, Wang et al., 2011, Inada et al., 2011, Hallermann et al., 2010, Chen and Ganetzky, 2012, Keegan et al., 2011, Tamura et al., 2011, Wairkar et al., 2009, Cauchi et al., 2008, Sanyal, 2009, Ratnaparkhi et al., 2008, Franciscovich et al., 2008, Wu et al., 2008, Wu et al., 2008, Fradkin et al., 2008, Higashi-Kovtun et al., 2010, Banovic et al., 2010, Bachmann et al., 2010, Xiong et al., 2010, Tsurudome et al., 2010, Liu et al., 2010, Pilgram et al., 2011, Merino et al., 2009, Freeman et al., 2011, Liu et al., 2011, Szul et al., 2011, James and Broihier, 2011, Ball et al., 2010, Beck et al., 2012, Nechipurenko and Broihier, 2012, Jansen et al., 2009, Akerboom et al., 2012, Chouhan et al., 2010)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference embryonic stage motor neuron (McCabe et al., 2003, Landgraf et al., 2003) larval stage Tv neuron \| subset (Marques et al., 2003) motor neuron (Marques et al., 2002, Marques et al., 2003, McCabe et al., 2004) larval stage -- pupal stage trachea \| restricted (Aberle et al., 2002) salivary gland (Aberle et al., 2002) wing disc (Aberle et al., 2002) motor neuron (Aberle et al., 2002) motor neuron (Kuppers-Munther et al., 2004) Comment:expression assayed in cultured cells
Additional Information
Statement Reference Expression is observed in only a few processes in cultured motor neurons. (Kuppers-Munther et al., 2004) Larval expression observed in motorneurons. (McCabe et al., 2004) Expression is observed in some or all Tv neurons in a randomly variable manner. (Marques et al., 2003) Expression observed in motor neurons from first larval instar through pupariation. (Aberle et al., 2002)

Marker for	embryonic motor neuron (McCabe et al., 2003) larval motor neuron (McCabe et al., 2004) larval motor neuron Tv neuron (Marques et al., 2003) motor neuron (Kuppers-Munther et al., 2004)
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	Insertion 34bp upstream of the Rapgap1 transcription start site (at position 7576638 of scaffold AE014134, genome release r5.13). (Sanyal, 2009)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	Gal4-OK6 (Bachmann et al., 2010) OK6 (McCabe et al., 2003, Merino et al., 2009, Cerrato et al., 2006, Sanyal, 2009, Tsurudome et al., 2010, Freeman et al., 2011, Keegan et al., 2011) OK6::Gal4 (Russo et al., 2009) OK6-Gal4 (Sanchez-Soriano et al., 2005, Kuppers-Munther et al., 2004, Landgraf et al., 2003, Marques et al., 2002, Aberle et al., 2002, Ataman, 2006, Ang et al., 2006, Parker et al., 2006, Banovic et al., 2010, Serpe and O'Connor, 2006, Franco et al., 2004, Wang et al., 2011, Pak et al., 2011, Tamura et al., 2011, Inada et al., 2011, Higashi-Kovtun et al., 2010, Xiong et al., 2010, Liu et al., 2010, Liu et al., 2011, Ball et al., 2010, Chen and Ganetzky, 2012, Chouhan et al., 2010, Beck et al., 2012, Akerboom et al., 2012) OK6-GAL4 (Hekmat-Scafe et al., 2005, Sherwood et al., 2004, McCabe et al., 2004, Bogdanik et al., 2004, Wang et al., 2007, Cauchi et al., 2008, Ratnaparkhi et al., 2008, Franciscovich et al., 2008, Pilgram et al., 2011, Chouhan et al., 2010, Jansen et al., 2009) ok6-GAL4 (Kittel et al., 2006, Hallermann et al., 2010, Owald et al., 2010) Ok6-GAL4 (Babcock et al., 2004) OK6GAL4 (Banerjee et al., 2004) OK6Gal4 (Nechipurenko and Broihier, 2012, Caygill and Johnston, 2008, James and Broihier, 2011) OK-6 GAL4 (Wairkar et al., 2009) OK6-gal4 (Wu et al., 2008, Wu et al., 2008) OK-Gal4 (van der Plas et al., 2006) Ok-GAL4 (Fradkin et al., 2008) P{GawB}OK6 (Marques et al., 2002, Aberle et al., 2002, McCabe et al., 2003, Marques et al., 2003, Kuppers-Munther et al., 2004, McCabe et al., 2004, FlyBase, 1992-, Szul et al., 2011, ) P<up>w+, OK6::Gal4</up> (Chouhan et al., 2010)
Secondary FlyBase IDs

References ( 60 )

Generate a list of	All references relating to this insertion ( 60 )

List References by type	Research paper ( 57 ) Supplementary material ( 2 ) FlyBase analysis ( 1 )
Recent research papers ( 14 )
	Akerboom et al., 2012, J. Neurosci. 32(40): 13819--13840 Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging. [FBrf0219564] Beck et al., 2012, J. Neurosci. 32(20): 7058--7073 Regulation of Fasciclin II and Synaptic Terminal Development by the Splicing Factor Beag. [FBrf0218385] Chen and Ganetzky, 2012, J. Cell Biol. 196(4): 529--543 A neuropeptide signaling pathway regulates synaptic growth in Drosophila. [FBrf0217500] Nechipurenko and Broihier, 2012, J. Cell Biol. 196(3): 345--362 FoxO limits microtubule stability and is itself negatively regulated by microtubule disruption. [FBrf0217391] Freeman et al., 2011, Mol. Cell. Neurosci. 46(2): 535--547 NFAT regulates pre-synaptic development and activity-dependent plasticity in Drosophila. [FBrf0212894] Inada et al., 2011, PLoS ONE 6(12): e29019 Optical dissection of neural circuits responsible for Drosophila larval locomotion with halorhodopsin. [FBrf0217087] James and Broihier, 2011, Development 138(15): 3273--3286 Crimpy inhibits the BMP homolog Gbb in motoneurons to enable proper growth control at the Drosophila neuromuscular junction. [FBrf0214390] Keegan et al., 2011, Nucleic Acids Res. 39(16): 7249--7262 Functional conservation in human and Drosophila of Metazoan ADAR2 involved in RNA editing: loss of ADAR1 in insects. [FBrf0215032] Liu et al., 2011, J. Neurosci. 31(6): 2052--2063 Drosophila Acyl-CoA Synthetase Long-Chain Family Member 4 Regulates Axonal Transport of Synaptic Vesicles and Is Required for Synaptic Development and Transmission. [FBrf0212968] Pak et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(30): 12390--12395 Mutation of the conserved polyadenosine RNA binding protein, ZC3H14/dNab2, impairs neural function in Drosophila and humans. [FBrf0214553] Pilgram et al., 2011, J. Neurosci. 31(2): 492--500 The RhoGAP crossveinless-c Interacts with Dystrophin and Is Required for Synaptic Homeostasis at the Drosophila Neuromuscular Junction. [FBrf0212767] Szul et al., 2011, Cell. Logist. 1(2): 69--76 The Garz Sec7 domain guanine nucleotide exchange factor for Arf regulates salivary gland development in Drosophila. [FBrf0213963] Tamura et al., 2011, PLoS ONE 6(11): e27408 Ku70 Alleviates Neurodegeneration in Drosophila Models of Huntington's Disease. [FBrf0216723] Wang et al., 2011, J. Clin. Invest. 121(10): 4118--4126 The ALS-associated proteins FUS and TDP-43 function together to affect Drosophila locomotion and life span. [FBrf0216240] Show all research papers ...