Dmel\P{SUPor-P}Sk2^KG05894 Insertion

General Information
Symbol	Dmel\P{SUPor-P}Sk2^KG05894	Species	D. melanogaster
Name		FlyBase ID	FBti0023507
Feature type	transposable_element_insertion_site
Description
Inserted element	P{SUPor-P}	Expression data
Affected gene(s)	Sk2	Viability / fertility	viable, fertile
Causes allele(s)	Sk2^KG05894	Stock availability	1 publicly available
LINE ID	KG05894
Genomic Location
Chromosomal location	3L ( 63A3 )	Sequence location	3L:3,047,135..3,047,135 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10
FB2012_01	References McElwain et al., 2011, PLoS ONE 6(11): e26993
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:3,047,135..3,047,135 [+] (Gene Disruption Project members, 2001-)
Orientation
Cytological location (computed by FlyBase)	63A3 ( inferred by FlyBase from sequence location )
Cytological location (reported)	63B5 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	BH840624 (Gene Disruption Project members, 2001-, FlyBase, 1992-) BH840625 (Gene Disruption Project members, 2001-, FlyBase, 1992-)
Inserted Element
Construct	P{SUPor-P}
Location-dependent role
Size	11.467Kb
Associated alleles	gypsy\su(Hw)BR⁴³⁰ w^BR.E.BR y^+mDint2
Molecular map
Affected Gene(s)
Insertion may affect gene	Sk2 (Gene Disruption Project members, 2001-, Yonamine et al., 2011)
Alleles and Phenotypes
Causes alleles	Sk2^KG05894 (Gene Disruption Project members, 2001-, Herr et al., 2004, Yonamine et al., 2011)

Lethality References viable (Gene Disruption Project members, 2001-, Herr et al., 2004, Yonamine et al., 2011)
Sterility References female sterile \| recessive (Herr et al., 2004) fertile (Gene Disruption Project members, 2001-, Yonamine et al., 2011)
Phenotype Manifest In

Detailed Description
Statement Reference One copy of Sk2[KG05894] fails to suppress the maternal effect lethality caused by expression of wntD[Scer\UAS.P\T.cEa] under the control of Scer\GAL4[Act.PU]. (McElwain et al., 2011) Sk2[KG05894] flies appear morphologically normal at room temperature. (Yonamine et al., 2011) Mutant animals show a marked increase in sphingolipid content at all stages of the life cycle compared to wild-type animals. Homozygotes show no reduction in viability at any stage of the life cycle and adult longevity is normal. Adults show impaired flight ability (20% reduction in flight performance), although the morphology and patterning of the indirect flight muscles appears normal. Homozygous females produce only 43% of the number of eggs that wild-type females produce. The ovaries do not show morphological defects and no abnormal masses other than an increase in fatty tissue that normally surrounds the gonads. The ovaries are consistently enlarged however, due to an increase in the number of mature eggs. (Herr et al., 2004)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	KG05894 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	14133 y¹ w^67c23; P{SUPor-P}Sk2^KG05894 ry⁵⁰⁶
Linkouts
Comments
	Location 3L:3030667-3030668 confirmed by FlyBase alignment of dbGSS accessions BH840625 and BH840624 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	KG05894 (Gene Disruption Project members, 2001-, FlyBase, 1992-) P{SUPor-P}CG2159^KG05894 (FlyBase, 1992-) P{SUPor-P}Sk2^KG05894 (FlyBase, 2005, FlyBase, 1992-) P{SUPor-P}Sphk2^KG05894 (FlyBase, 1992-) Sk2^KG05894 (Yonamine et al., 2011)
Secondary FlyBase IDs

References ( 7 )
Research paper	McElwain et al., 2011, PLoS ONE 6(11): e26993 A suppressor/enhancer screen in Drosophila reveals a role for wnt-mediated lipid metabolism in primordial germ cell migration. [FBrf0216663] Yonamine et al., 2011, J. Cell Biol. 192(4): 557--567 Sphingosine kinases and their metabolites modulate endolysosomal trafficking in photoreceptors. [FBrf0213095] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Herr et al., 2004, J. Biol. Chem. 279(13): 12685--12694 Characterization of the Drosophila sphingosine kinases and requirement for Sk2 in normal reproductive function. [FBrf0174774]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177]

Dmel\P{SUPor-P}Sk2KG05894 Insertion

Dmel\P{SUPor-P}Sk2^KG05894 Insertion