Dmel\P{SUPor-P}Sox15^KG09145 Insertion

General Information
Symbol	Dmel\P{SUPor-P}Sox15^KG09145	Species	D. melanogaster
Name		FlyBase ID	FBti0024248
Feature type	transposable_element_insertion_site
Description
Inserted element	P{SUPor-P}	Expression data
Affected gene(s)	Sox15	Viability / fertility
Causes allele(s)	Sox15^KG09145	Stock availability	2 publicly available
LINE ID	KG09145
Genomic Location
Chromosomal location	2R ( 50E4 )	Sequence location	2R:10,096,285..10,096,285 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{SUPor-P}.KG A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{SUPor-P}; created and vetted by the Gene Disruption Project (GDP). The P{SUPor-P} construct carries two visible markers: a w[+mC] mini-white marker flanked by su(Hw)-binding sites, designed to reduce the variablity of the phenotype of the white gene marker due position effects, in addition to the mini-yellow marker y[+mDint2]. (Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:10,096,285..10,096,285 [-] (Gene Disruption Project members, 2001-)
Orientation
Cytological location (computed by FlyBase)	50E4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	50E6 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	BZ286333 (Gene Disruption Project members, 2001-, FlyBase, 1992-)
Inserted Element
Construct	P{SUPor-P}
Location-dependent role
Size	11.467Kb
Associated alleles	gypsy\su(Hw)BR⁴³⁰ w^BR.E.BR y^+mDint2
Molecular map
Affected Gene(s)
Insertion may affect gene	Sox15 (Gene Disruption Project members, 2001-, Miller et al., 2009, Dichtel-Danjoy et al., 2009)
Alleles and Phenotypes
Causes alleles	Sox15^KG09145 (Gene Disruption Project members, 2001-, Miller et al., 2009, Dichtel-Danjoy et al., 2009)

Lethality References partially lethal - majority die \| recessive (Dichtel-Danjoy et al., 2009)
Sterility References
Phenotype Manifest In
	alula (Dichtel-Danjoy et al., 2009) wing hinge (Dichtel-Danjoy et al., 2009) wing hinge primordium (Dichtel-Danjoy et al., 2009) wing sclerite (Dichtel-Danjoy et al., 2009)
Detailed Description
Statement Reference In wg[spd-fg], Sox15[KG09145] double mutant third instar larval wing imaginal discs, the distal hinge region is reduced in size compared to either single mutant. The rate of hinge-specific apoptosis is similar in wg[spd-fg], Sox15[KG09145] double mutant and Sox15[KG09145] single mutant L3 wing discs. (Dichtel-Danjoy et al., 2009) Most homozygous Sox15[KG09145] flies die as pharate adults. The homozygous Sox15[KG09145] escapers are weak and display held-out wings. The held-out-wing phenotype is the result of abnormalities in proximal hinge structures, such as the sclerites, the alula and the costa. Sox15[KG09145] mutant third instar larval wing imaginal discs show hinge overgrowth that causes extra folds both dorsally and ventrally. There is an increase in cell proliferation in the hinge region of Sox15[KG09145] mutant L3 wing imaginal discs relative to the wild-type. The rate of apoptotic cell death is also increased in Sox15[KG09145] mutant wing discs in the hinge region. The wing pouch and body wall regions appear unaffected in Sox15[KG09145] mutant third instar larval wing imaginal discs. (Dichtel-Danjoy et al., 2009)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	KG09145 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	14980 y¹; P{SUPor-P}Sox15^KG09145/CyO; ry⁵⁰⁶
Kyoto	114403 y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{neoFRT}42D P{SUPor-P}Sox15^KG09145 /CyO y⁺
Linkouts
Comments
	Location 2R:9723643-9723644 confirmed by FlyBase alignment of dbGSS accession BZ286333 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	KG09145 (Gene Disruption Project members, 2001-, FlyBase, 1992-, Miller et al., 2009, Dichtel-Danjoy et al., 2009) P{SUPor-P}Sox15^KG09145 (FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 7 )
Research paper	Dichtel-Danjoy et al., 2009, Development 136(5): 761--769 SoxF is part of a novel negative-feedback loop in the wingless pathway that controls proliferation in the Drosophila wing disc. [FBrf0206770] Miller et al., 2009, Dev. Biol. 329(2): 386--399 Complex interplay of three transcription factors in controlling the tormogen differentiation program of Drosophila mechanoreceptors. [FBrf0207969] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]
FlyBase analysis	FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{SUPor-P}Sox15KG09145 Insertion

Dmel\P{SUPor-P}Sox15^KG09145 Insertion