Dmel\P{Mae-UAS.6.11}Rheb^AV4 Insertion

General Information
Symbol	Dmel\P{Mae-UAS.6.11}Rheb^AV4	Species	D. melanogaster
Name		FlyBase ID	FBti0024400
Feature type	transposable_element_insertion_site
Description
Inserted element	P{Mae-UAS.6.11}	Expression data
Affected gene(s)	Rheb	Viability / fertility
Causes allele(s)	Rheb^AV4	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	3R ( 83B2 )	Sequence location
Recent Updates
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FB2011_10
FB2012_01
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	83B2 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{Mae-UAS.6.11}
Location-dependent role	mobile activating element (UASG) (Merriam, 1997.5.9)
Size
Associated alleles	Scer\UAS^5x.hs y^+t7.7
Molecular map
Affected Gene(s)
Insertion may affect gene	Rheb (Patel et al., 2003, Scott et al., 2007, Scott et al., 2007, Chang and Neufeld, 2009, Chang and Neufeld, 2009, Parrish et al., 2009, Khurana et al., 2006)
Alleles and Phenotypes
Causes alleles	Rheb^AV4 (Patel et al., 2003, Patel et al., 2003, Goberdhan et al., 2005, Scott et al., 2007, Scott et al., 2007, Chang and Neufeld, 2009, Chang and Neufeld, 2009, Parrish et al., 2009, Khurana et al., 2006)

Lethality References lethal (Patel et al., 2003) lethal \| larval stage \| recessive (Patel et al., 2003) lethal \| recessive \| second instar larval stage (Patel et al., 2003)
Sterility References
Phenotype Manifest In
	antenna (Patel et al., 2003) embryonic/larval hindgut (Patel et al., 2003) eye (Patel et al., 2003, Goberdhan et al., 2005) eye \| somatic clone (Patel et al., 2003) head (Patel et al., 2003) head \| somatic clone (Patel et al., 2003) interommatidial bristle \| supernumerary (Patel et al., 2003) ommatidium (Patel et al., 2003) ommatidium \| somatic clone (Patel et al., 2003)
Detailed Description
Statement Reference The elimination of cells expressing Atg1[Scer\UAS.cSa] under the control of Scer\GAL4[Act5C.PP] from the wing disc is delayed by co-expression of Rheb[AV4]. (Scott et al., 2007) The rough-eye phenotype observed in animals expressing Hsap\MAPT[V337M.Scer\UAS] under the control of Scer\GAL4[GMR.PF] is enhanced by co-expression of Rheb[AV4]. This enhancement is blocked in a Cdk4[3]/+ background, or by co-expressing dap[Scer\UAS.cdNa] and Rbf[Scer\UAS.cDa]. The rough-eye phenotype observed in animals expressing Hsap\MJD[tr.Q78.Scer\UAS.T:Ivir\HA1] in the eye under the control of Scer\GAL4[GMR.PF] is not modified by co-expression of Rheb[AV4]. (Khurana et al., 2006) Expression of Rheb^AV4, under the control of Scer\GAL4^GMR.PF, produces a greatly enlarged eye. (Goberdhan et al., 2005) Co-expression of Rheb^AV4 results in a strong enhancement of Scer\GAL4^GMR.PF>foxo^GS9928, leading to loss of virtually all ommatidia in females and pupal lethality in males with massive tissue degeneration in the head of late pupae. These phenotypes are partially suppressed by Akt1^Scer\UAS.cSa overexpression. (Goberdhan et al., 2005) Lethality acts in the second larval instar. Generation of clones of cells in the eye disc that overexpress DRheb results in enlarged eyes and head, while generation of loss-of-function clones in a sensitized (Minute) background results in a dramatic reduction of eye and head size. (Patel et al., 2003) Expression of Rheb^AV4 driven by Scer\GAL4^byn-Gal4 causes lethality. This expression also causes a dramatically enlarged (both in width and length) hindgut in the first instar larva. Expression of Rheb^AV4 driven by Scer\GAL4^GMR.PF results in marked overgrowth of the eye compared with wild type. Expression of Rheb^AV4 driven by Scer\GAL4^Act5C.PI results in an enlarged eye, antenna and head. When compared to wild-type eyes, the ommatidia are much larger, are not organized into the normal hexagonal array and are frequently flanked by extra bristles. These bristles are thicker than in wild type. Embryos homozygous for Rheb^AV4 hatch into first instar larvae that grow very slowly, move lethargically, and die by 72 hours without molting into second instar larvae. Heads and eyes containing multiple Rheb^AV4 homozygous clones are dramatically reduced in size compared to those containing wild-type clones. Sections of eyes with Rheb^AV4 homozygous clones show disorganized, smaller ommatidia composed of smaller cells. Heterozygous Rheb^AV4 larvae usually emerge from the pupal case at the same time as in wild type. In the presence of rapamycin, however, Rheb^AV4 heterozygotes emerge two days later than their wild-type siblings. (Patel et al., 2003)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	9690 y¹ w^*; P{Mae-UAS.6.11}Rheb^AV4/TM6B, Tb¹
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{Mae-UAS.6.11}Rheb^AV4 (FlyBase, 1992-) P{yUAS}Rheb^AV4 (Patel et al., 2003, FlyBase, 1992-) Rheb^AV4 (Khurana et al., 2006) UAS-Rheb (Chang and Neufeld, 2009) UAS-Rheb^AV4 (Scott et al., 2007, Scott et al., 2007, Chang and Neufeld, 2009) unnamed (Parrish et al., 2009)
Secondary FlyBase IDs

References ( 11 )
Research paper	Chang and Neufeld, 2009, Mol. Biol. Cell 20(7): 2004--2014 An Atg1/Atg13 complex with multiple roles in TOR-mediated autophagy regulation. [FBrf0207848] Parrish et al., 2009, Neuron 63(6): 788--802 The microRNA bantam functions in epithelial cells to regulate scaling growth of dendrite arbors in drosophila sensory neurons. [FBrf0208858] Scott et al., 2007, Curr. Biol. 17(1): 1--11 Direct induction of autophagy by Atg1 inhibits cell growth and induces apoptotic cell death. [FBrf0194462] Khurana et al., 2006, Curr. Biol. 16(3): 230--241 TOR-mediated cell-cycle activation causes neurodegeneration in a Drosophila tauopathy model. [FBrf0190026] Goberdhan et al., 2005, Development 132(10): 2365--2375 PAT-related amino acid transporters regulate growth via a novel mechanism that does not require bulk transport of amino acids. [FBrf0187461] Patel et al., 2003, J. Cell Sci. 116(17): 3601--3610 Drosophila Rheb GTPase is required for cell cycle progression and cell growth. [FBrf0162194]
Supplementary material	Chang and Neufeld, 2009, Mol. Biol. Cell 20(7): Supplemental Data. [FBrf0208115] Scott et al., 2007, Curr. Biol. 17(1): Supplemental Data. [FBrf0208086]
Personal communication to FlyBase	Merriam, 1997.5.9, Molecular and genetic information on a P construct. Molecular and genetic information on a P construct. [FBrf0093303]
Abstract	Patel et al., 2003, A. Dros. Res. Conf. 44: 182B Drosophila Rheb is required for growth of the eye imaginal disc. [FBrf0154438]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]

Dmel\P{Mae-UAS.6.11}RhebAV4 Insertion

Dmel\P{Mae-UAS.6.11}Rheb^AV4 Insertion