Dmel\P{GT1}Lac^BG01462 Insertion

General Information
Symbol	Dmel\P{GT1}Lac^BG01462	Species	D. melanogaster
Name		FlyBase ID	FBti0024426
Feature type	transposable_element_insertion_site
Description
Inserted element	P{GT1}	Expression data
Affected gene(s)	Lac	Viability / fertility
Causes allele(s)	Lac^BG01462	Stock availability	2 publicly available
LINE ID	BG01462
Genomic Location
Chromosomal location	2R ( 49A6 )	Sequence location	2R:8,340,753..8,340,753 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:8,340,753..8,340,753 [+] (Gene Disruption Project members, 2001-)
Orientation
Cytological location (computed by FlyBase)	49A6 ( inferred by FlyBase from sequence location )
Cytological location (reported)	49A7 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	BH901112 (Gene Disruption Project members, 2001-, FlyBase, 1992-)
Inserted Element
Construct	P{GT1}
Location-dependent role	GAL4 gene trap (Lukacsovich and Asztalos, 1999.7.15)
Size	8.447, 8.447Kb
Associated alleles	Scer\GAL4^GT1 Tn\neoR^Hsp70Bb.PS w^+mGT
Molecular map
Affected Gene(s)
Insertion may affect gene	Lac (Gene Disruption Project members, 2001-)
Alleles and Phenotypes
Causes alleles	Lac^BG01462 (Gene Disruption Project members, 2001-)

Lethality References lethal (Llimargas et al., 2004) lethal \| embryonic stage (Llimargas et al., 2004)
Sterility References
Phenotype Manifest In
	dorsal group branch precursor (Llimargas et al., 2004) dorsal trunk primordium (Llimargas et al., 2004) lateral group branch precursor (Llimargas et al., 2004) presumptive embryonic/larval tracheal system (Llimargas et al., 2004) presumptive embryonic salivary gland (Llimargas et al., 2004) tracheocyte \| embryonic stage (Llimargas et al., 2004)
Detailed Description
Statement Reference In Lac^BG01462 homozygous embryos, the early events of tracheal development, such as guidance, primary branching, branch fusion and extension of terminal branches occur normally. However, from stage 15 onwards most branches become more sinuous or convoluted than in the wild-type; this is especially conspicuous in the dorsal trunk. The convoluted shape of the tracheal tubes is a consequence of having lengthened too much. However, this extra growth does not appear to derive from an increase in the number of embryonic tracheocytes, but rather from an increase in the length of the cells. Tracheal lumens in these embryos are uneven, particularly those of the dorsal and lateral branches, with expansions, constrictions and numerous lumenal breaks and discontinuities. Many lumenal breaks are the result from detachment of tracheal branches from one another. Cell detachments that break the tracheal tubes are found particularly in those branches where cells elongate more. Tracheal tubes in these embryos do not inflate at the end of embryogenesis. Trachea in late stage Lac^BG01462 homozygous embryos retain normal epithelial polarity, and normal polarization of the cytoskeleton. However, dye permeability assays show that these embryos are unable to establish or maintain the tracheal diffusion barrier. In these assays, dye is also internalized in the salivary glands, a phenotype not seen in wild-type. At late embryonic stages these embryos have necrotic tissue in the salivary gland region. (Llimargas et al., 2004)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	BG01462 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	14577 w¹¹¹⁸; P{GT1}Lac^BG01462/CyO
Kyoto	114392 y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{neoFRT}42D P{GT1}Lac^BG01462 /CyO y⁺
Linkouts
Comments
	Location 2R:7969155-7969156 confirmed by FlyBase alignment of dbGSS accession BH901112 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	BG01462 (Gene Disruption Project members, 2001-, FlyBase, 1992-) P{GT1}Lac^BG01462 (FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 6 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Llimargas et al., 2004, Development 131(1): 181--190 Lachesin is a component of a septate junction-based mechanism that controls tube size and epithelial integrity in the Drosophila tracheal system. [FBrf0167526]
Personal communication to FlyBase	Lukacsovich and Asztalos, 1999.7.15, Dual tagging gene-trap of novel genes in Drosophila melanogaster. Dual tagging gene-trap of novel genes in Drosophila melanogaster. [FBrf0108534]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177]

Dmel\P{GT1}LacBG01462 Insertion

Dmel\P{GT1}Lac^BG01462 Insertion