A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{GT1}flamBG02658 Insertion

General Information
Symbol Dmel\P{GT1}flamBG02658 Species D. melanogaster
Name FlyBase ID FBti0024429
Feature type transposable_element_insertion_site
Description
Inserted element P{GT1} Expression data
Affected gene(s) flam Viability / fertility viable, fertile
Causes allele(s) flamBG02658 Stock availability 1 publicly available
LINE ID BG02658
Genomic Location
Chromosomal location X ( 20A5 ) Sequence location X:21,502,538..21,502,538 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0060088 FBti0019699 FBti0060105 FBti0060087 FBti0019700 FBti0019701 FBti0060136 FBti0019702 FBti0060065 FBti0060066 FBti0019704 FBti0059698 FBti0042005 FBti0076267 FBti0108630 FBti0027147 FBti0024429 FBti0071571 FBti0066837 FBti0039027 FBti0147227 FBti0068527
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GT1}; created and vetted by the Gene Disruption Project (GDP). The P{GT1} construct is described as a dual-tagged gene trap (FBrf0132344):it carries (1) w[+mGT1] mini-white marker, designed to confer a pigmented eye phenotype only if the construct is inserted within a host gene, and (2) a promoter-less Ser\GAL4 gene which is expressed if it is produced as a fusion with the mRNA produced by the host gene. The construct also carries a Tn\neoR selectable marker (FBrf0132344).
(Bellen et al., 2004)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
X:21,502,538..21,502,538 [-]
(Gene Disruption Project members, 2001-)
Orientation
Cytological location
(computed by FlyBase)
20A5 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
20A (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
BH854697
(Gene Disruption Project members, 2001-, FlyBase, 1992-)
hide Inserted Element
Construct P{GT1}
Location-dependent
role
GAL4 gene trap
(Lukacsovich and Asztalos, 1999.7.15)
Size 8.447, 8.447Kb
Associated alleles
Scer\GAL4GT1
Tn\neoRHsp70Bb.PS
w+mGT
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
flam
(Mevel-Ninio et al., 2007, Brennecke et al., 2007)
hide Alleles and Phenotypes
Causes alleles
flamBG02658
(Mevel-Ninio et al., 2007, Brennecke et al., 2007)
Lethality
References
viable
(Gene Disruption Project members, 2001-)
Sterility
References
female sterile
(Mevel-Ninio et al., 2007)
fertile
(Gene Disruption Project members, 2001-)
hide Phenotype Manifest In
dorsal appendage
(Mevel-Ninio et al., 2007)
egg
(Mevel-Ninio et al., 2007)
egg chamber
(Mevel-Ninio et al., 2007)
germline cell
(Mevel-Ninio et al., 2007)
ovariole
(Mevel-Ninio et al., 2007)
ovary
(Mevel-Ninio et al., 2007, Zamparini et al., 2011)
hide Detailed Description
Statement
Reference
flam[BG02658]/flam[KG00476] ovaries show defective germline and soma differentiation phenotypes. Somatic cells also die.
(Zamparini et al., 2011)
Homozygous females do not lay any eggs. flamBG02658 flies have a permissive phenotype with respect to gypsy expression (assayed by expression of a P{gypsy-lacZ.p12} reporter construct in the ovaries). flamBG02658 flies are unstable for ZAM expression (assayed by expression of a P{ZAM-lacZ.LTR} reporter construct in the ovaries). 24 hour old flamBG02658 females have ovaries which are very small and contain rudimentary ovarioles within which no differentiation of germ cells has taken place. Ovarioles of 48 hour old mutant females are severely dystrophic, containing two to three completely disorganised egg chambers which contain a reduced number of abnormally shaped nuclei. Mature eggs are rarely formed, and those that do are smaller than normal and appear to lack dorsal-ventral and anterior-posterior polarities. The dorsal appendages are short and abnormally shaped. The follicular epithelium is completely disorganised in the ovaries of 6-day old mutant females. Mosaic females containing homozygous germline clones develop several completely normal ovarioles and are fertile. The eggs produced by these females give rise to normal adult flies.
(Mevel-Ninio et al., 2007)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
BG02658
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
13912
w1118 P{GT1}flamBG02658
hide Linkouts
hide Comments
Location X:21442186-21442188 confirmed by FlyBase alignment of dbGSS accession BH854697 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
BG02658
(Gene Disruption Project members, 2001-, Mevel-Ninio et al., 2007)
P(BG02658)
(Brennecke et al., 2007)
P(flamBG)
(Brennecke et al., 2007)
P{GT1}BG02658
(FlyBase, 2005, FlyBase, 1992-)
P{GT1}flamBG02658
(FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Zamparini et al., 2011, Development 138(18): 4039--4050
Vreteno, a gonad-specific protein, is essential for germline development and primary piRNA biogenesis in Drosophila. [FBrf0214783]
Brennecke et al., 2007, Cell 128(6): 1089--1103
Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila. [FBrf0200494]
Mevel-Ninio et al., 2007, Genetics 175(4): 1615--1624
The flamenco locus controls the gypsy and ZAM retroviruses and is required for Drosophila oogenesis. [FBrf0200369]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
Lukacsovich and Asztalos, 1999.7.15, Dual tagging gene-trap of novel genes in Drosophila melanogaster.
Dual tagging gene-trap of novel genes in Drosophila melanogaster. [FBrf0108534]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]