A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{SUPor-P}plexBKG00878 Insertion

General Information
Symbol Dmel\P{SUPor-P}plexBKG00878 Species D. melanogaster
Name FlyBase ID FBti0024432
Feature type transposable_element_insertion_site
Description
Inserted element P{SUPor-P} Expression data
Affected gene(s) plexB Viability / fertility
Causes allele(s) plexBKG00878 Stock availability 1 publicly available
LINE ID KG00878
Genomic Location
Chromosomal location 4 ( 101F1 ) Sequence location 4:64,361..64,361 [+]
Map ( GBrowse ) detailed view FBti0020404 FBti0020405 FBti0062217 FBti0062808 FBti0062218 FBti0019472 FBti0065488 FBti0047547 FBti0024432
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
4:64,361..64,361 [+]
(Gene Disruption Project members, 2001-)
Orientation
Cytological location
(computed by FlyBase)
Cytological location
(reported)
101F1 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
BH900861
(Gene Disruption Project members, 2001-, FlyBase, 1992-)
hide Inserted Element
Construct P{SUPor-P}
Location-dependent
role
Size 11.467Kb
Associated alleles
gypsy\su(Hw)BR430
wBR.E.BR
y+mDint2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
plexB
(Gene Disruption Project members, 2001-, FlyBase Curators, 2008)
hide Alleles and Phenotypes
Causes alleles
plexBKG00878
(FlyBase Curators, 2008)
Lethality
References
semi-lethal
(Ayoob et al., 2006)
Sterility
References
hide Phenotype Manifest In
intersegmental nerve
(Ayoob et al., 2006)
medial longitudinal fascicle
(Ayoob et al., 2006)
SNa
(Ayoob et al., 2006)
hide Detailed Description
Statement
Reference
plexB[KG00878] fails to complement Df(4)M101-62f with respect to the plexB[KG00878] lethality phenotype. Expression of plexB[Scer\UAS.cHa] in all neurons in a plexB[KG00878] homozygous background, under the control of Scer\GAL4[elav-C155], significantly rescues the intersegmental nerve b (ISNb) motor axon defects. Restoring plexB to all neurons also rescues the lethality associated with the plexB[KG00878] mutants (16.8% homozygous viable progeny compared to 5.7% of the plexB[KG00878]/+ x Df(4)M101-62f/+ crossing being homozygous viable progeny). Expression of plexB[Scer\UAS.cHa] in all neurons in a plexB[KG00878] homozygous background, under the control of Scer\GAL4[elav-C155], significantly reduces the number of SNa defects by over 50%. Expression of plexB[Scer\UAS.cHa] in all neurons in a plexB[KG00878] homozygous background, under the control of Scer\GAL4[elav-C155] rescues the severe defasciculation of the medial Fas2-stained longitudinal fascicle.
(Ayoob et al., 2006)
plexB[KG00878] is semi-lethal and yields a small percentage (approximately 5.7%) of homozygous adult flies that are unable to mate successfully. plexB[KG00878] homozygous embryos exhibit highly penetrant defects in axon guidance. Motor axons that contribute to the intersegmental nerve, the segmental nerve, and a subset of longitudinally projecting CNS axons, fail to defasciculate from one another. plexB[KG00878] homozygous embryos do not exhibit defects in overall muscle morphology. Motor axons in the Segmental nerve a (SNa) pathway are affected in plexB[KG00878] mutant embryos. In approximately almost 50% of all plexB[KG00878] hemisegments the anterior of the SNa dorsal branch incorrectly projects between muscles 21 and 22 instead of more posteriorly between muscles 22 and 23. In approximately 33.1% of all hemisegments the anteriorly misprojecting SNa motor axons take the wrong path but subsequently make two turns to reach their proper target. In approximately 14.7% of all plexB[KG00878] hemisegments the anteriorly projecting SNa dorsal branch takes the wrong path and is unable to reach its proper target. plexB[KG00878] homozygous embryos exhibit defects in the innervation of the ventral muscles in most of the segments examined. The medial Fas2-stained longitudinal fascicle in plexB[KG00878] mutants is severely defasciculated along its length. plexB[KG00878]/Df(4)M101-62f double heterozygous embryos exhibit phenotypes that are virtually identical to those observed in homozygous plexB[KG00878] embryos.
(Ayoob et al., 2006)
Expression of plexA[Scer\UAS.T:Ivir\HA1] in a plexB[KG00878] mutant background rescues the total number of ISNb defasciculation defects by 25%, and the more severe ISNb bypass phenotypes by almost 50%. plexA[Scer\UAS.T:Ivir\HA1] expression does not rescue the \'double turn\' or \'lost\' phenotypes of plexB[KG00878] mutants. plexA[Scer\UAS.T:Ivir\HA1] expression reduces the incidence of plexB[KG00878] SNa \'stall\' phenotypes in plexB[KG00878] mutants by 20%. However, it does not reduce the total fraction of defective SNa pathways and is incapable of rescuing plexB[KG00878] CNS phenotypes. Expression of plexA[Scer\UAS.T:Ivir\HA1] also provides a modest reduction in the lethality observed in plexB[KG00878] mutants. Approximately 58%of Df(3R)swp2[MICAL]/+; plexB[KG00878]/+ double transheterozygous embryonic hemisegments exhibit ISNb defects. This penetrance is equivalent to that seen for the same ISNb defects in plexB[KG00878] homozygous mutants. A plexB[KG00878] background greatly reduces the tranverse nerve phenotype found in Sema-2a[Scer\UAS.cKa]; Scer\GAL4[how-24B] mutants (from aberrant formation in 27.9% to 12.2% of hemisegments).
(Ayoob et al., 2006)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
KG00878
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
14579
y1; ry506; P{SUPor-P}plexBKG00878/ciD
hide Linkouts
hide Comments
Location 4:64361-64362 confirmed by FlyBase alignment of dbGSS accession BH900861 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
KG00878
(Gene Disruption Project members, 2001-)
P{SUPor-P}KG00878
(FlyBase, 2005, FlyBase, 1992-)
P{SUPor-P}plexBKG00878
(FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 6 )
Research paper
Ayoob et al., 2006, Development 133(11): 2125--2135
Drosophila Plexin B is a Sema-2a receptor required for axon guidance. [FBrf0190270]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
FlyBase analysis
FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations.
Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]