Dmel\P{SUPor-P}Mdr65^KG08723 Insertion

General Information
Symbol	Dmel\P{SUPor-P}Mdr65^KG08723	Species	D. melanogaster
Name		FlyBase ID	FBti0024796
Feature type	transposable_element_insertion_site
Description
Inserted element	P{SUPor-P}	Expression data
Affected gene(s)	Mdr65	Viability / fertility	viable, fertile
Causes allele(s)	Mdr65^KG08723	Stock availability	1 publicly available
LINE ID	KG08723
Genomic Location
Chromosomal location	3L ( 65A10 )	Sequence location	3L:6,236,128..6,236,128 [-]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:6,236,128..6,236,128 [-] (Gene Disruption Project members, 2001-)
Orientation
Cytological location (computed by FlyBase)	65A10 ( inferred by FlyBase from sequence location )
Cytological location (reported)	65A7 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	BH901069 (Gene Disruption Project members, 2001-, FlyBase, 1992-)
Inserted Element
Construct	P{SUPor-P}
Location-dependent role
Size	11.467Kb
Associated alleles	gypsy\su(Hw)BR⁴³⁰ w^BR.E.BR y^+mDint2
Molecular map
Affected Gene(s)
Insertion may affect gene	Mdr65 (Gene Disruption Project members, 2001-, Mayer et al., 2009)
Alleles and Phenotypes
Causes alleles	Mdr65^KG08723 (Gene Disruption Project members, 2001-, Mayer et al., 2009)

Lethality References viable (Gene Disruption Project members, 2001-)
Sterility References fertile (Gene Disruption Project members, 2001-)
Phenotype Manifest In

Detailed Description
Statement Reference Mdr65[KG08723] mutant flies show high relative brain-specific accumulation of Rhodamine B dye, suggesting that Mdr65 promotes efflux of xenobiotics from the brain. Whole animal controls show that dosing and elimination of Rhodamine B are not affected by the Mdr65[KG08723] mutation. Brain accumulation of 3kDa FITC-dextran remains near background levels in both wild-type and Mdr65[KG08723] animals, indicating that the P{SUPor-P}Mdr65[KG08723] insertion does not significantly disturb the paracellular diffusion barrier. The blood-brain barrier in Mdr65[KG08723] mutants is indistinguishable from wild-type relative to all highly-charged small molecules tested. Brain levels of Rhodamine B are similar between Mdr65[KG08723] and wild-type flies at early time points, showing that the P{SUPor-P}Mdr65[KG08723] insertion does not affect initial brain penetration by Rhodamine B. At 4, 8, and 16 hours after injection, however, Mdr65[KG08723] mutant flies exhibit significantly higher brain levels of Rhodamine B than wild-type flies, suggesting the mutant flies have a markedly reduced Rhodamine B efflux from the brain. (Mayer et al., 2009)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	KG08723 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	14757 y¹ w^67c23; P{SUPor-P}Mdr65^KG08723 ry⁵⁰⁶
Linkouts
Comments
	Location 3L:6216975-6216976 confirmed by FlyBase alignment of dbGSS accession BH901069 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	KG08723 (Gene Disruption Project members, 2001-, FlyBase, 1992-, Mayer et al., 2009) P{SUPor-P}Mdr65^KG08723 (FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 5 )
Research paper	Mayer et al., 2009, J. Neurosci. 29(11): 3538--3550 Evolutionary conservation of vertebrate blood-brain barrier chemoprotective mechanisms in Drosophila. [FBrf0207483] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177]

Dmel\P{SUPor-P}Mdr65KG08723 Insertion

Dmel\P{SUPor-P}Mdr65^KG08723 Insertion