Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w+mC and the mini-yellow marker y+mDint2, and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Third instar larvae expressing P{EPgy2}CR43669EY00118 under the control of Scer\GAL4Pxn.PS have an increased number of hemocytes under the cuticle. The number of hemocytes in circulation is similar to wild type. The dorsal sessile hemocyte department is not disrupted. Hemocytes do not accumulate along the dorsal vessel or spread through the cuticle. The lymph gland is not enlarged. Hemocyte shape is normal.
Location 2L:16696703-16696704 confirmed by FlyBase alignment of dbGSS accession BZ748755 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.