A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EPgy2}InREY00681 Insertion

General Information
Symbol Dmel\P{EPgy2}InREY00681 Species D. melanogaster
Name FlyBase ID FBti0025149
Feature type transposable_element_insertion_site
Description
Inserted element P{EPgy2} Expression data
Affected gene(s) InR Viability / fertility viable, fertile
Causes allele(s) InREY00681 Stock availability 1 publicly available
LINE ID EY00681
Genomic Location
Chromosomal location 3R ( 93E6 ) Sequence location 3R:17,431,542..17,431,542 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0059759 FBti0020383 FBti0044664 FBti0150260 FBti0067978 FBti0048641 FBti0048120 FBti0045303 FBti0112219 FBti0112811 FBti0036017 FBti0050324 FBti0107520 FBti0106081 FBti0074699 FBti0054803 FBti0025149 FBti0105039 FBti0028171 FBti0107324 FBti0068233 FBti0066598 FBti0050045 FBti0011433 FBti0027292 FBti0054658 FBti0067021 FBti0066631 FBti0103831
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w[+mC] and the mini-yellow marker y[+mDint2], and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Bellen et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:17,431,542..17,431,542 [-]
(Gene Disruption Project members, 2001-)
Orientation
Cytological location
(computed by FlyBase)
93E6 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
93E11 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
BZ748780
(Gene Disruption Project members, 2001-, FlyBase, 1992-)
hide Inserted Element
Construct P{EPgy2}
Location-dependent
role
mobile activating element (UASG)
(Bellen et al., 2004)
Size 10.908Kb
Associated alleles
Scer\UAS14x.P\T
TrlBRcRa
w+mC
y+mDint2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
InR
(Zhao et al., 2008)
hide Alleles and Phenotypes
Causes alleles
InREY00681
(Zhao et al., 2008)
Lethality
References
viable
(Gene Disruption Project members, 2001-)
Sterility
References
fertile
(Gene Disruption Project members, 2001-)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EY00681
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
15306
y1 w67c23; P{EPgy2}InREY00681
hide Linkouts
hide Comments
Location 3R:17431542-17431543 confirmed by FlyBase alignment of dbGSS accession BZ748780 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EY00681
(Gene Disruption Project members, 2001-)
EY(3)00681
(Zhao et al., 2008)
P{EPgy2}EY00681
(FlyBase, 2005, FlyBase, 1992-)
P{EPgy2}InREY00681
(FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 7 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Zhao et al., 2008, Genetics 178(2): 883--901
A Drosophila gain-of-function screen for candidate genes involved in steroid-dependent neuroendocrine cell remodeling. [FBrf0204350]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]