Dmel\P{lacW}k08901b Insertion

General Information
Symbol	Dmel\P{lacW}k08901b	Species	D. melanogaster
Name		FlyBase ID	FBti0025322
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	CG10764, Ecol\lacZ, mthl3	Viability / fertility
Causes allele(s)	Ecol\lacZ^k08901b	Stock availability	2 publicly available
LINE ID	l(2)k08901
Genomic Location
Chromosomal location	2R ( 54B16 )	Sequence location	2R:13,340,212..13,340,212 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{lacW}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800). Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:13,340,212..13,340,212 [+]
Orientation
Cytological location (computed by FlyBase)	54B16 ( inferred by FlyBase from sequence location )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence	AQ034114 (FlyBase, 1992-, Levis, 2003.1.30, BDGP Project Members, 1994-1999)
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	CG10764 (FlyBase, 1992-) Ecol\lacZ (BDGP Project Members, 1994-1999) mthl3 (Levis, 2003.1.30)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^k08901b (BDGP Project Members, 1994-1999)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k08901 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration	Df(2R)k08901 (Barbash, 2011.12.8)
Balancer
Stocks ( 2 )
Bloomington	10836 y¹ w^67c23; Df(2R)k08901, P{lacW}l(2)k01209^k08901a P{lacW}k08901b/CyO
Kyoto	111251 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}l(2)k01209^k08901a P{lacW}k08901b /CyO y⁺
Linkouts
Comments
	Location 2R:12967569-12967570 determined by FlyBase alignment of dbGSS accession AQ034114 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005) Two insertions present, 30kb apart, or one insertion associated with a 30kb deletion. (Levis, 2003.1.30) FlyBase curator comment: The "k08901" insertion line contains a deletion (Df(2R)k08901) (see FBrf0217415). It is not clear how many insertions are present in this line: although the 5' (AQ034114) and 3' (AQ025874) flanking sequences map 30kb apart, consistent with the presence of the deletion, the reported location of the P-element insertion site in the two flanking sequences appear to be incompatible if only one insertion is present. It is possible that there is one insertion in the line and that there is an error in the reported sequence data, or there may be two insertions in the line. Until more information is known, a separate insertion record exists in FlyBase for each flanking sequence: P{lacW}k08901b (FBti0025322) corresponds to the flanking sequence reported in AQ034114, P{lacW}l(2)k01209[k08901a] (FBti0006619) corresponds to the flanking sequence reported in AQ025874. (FlyBase, 1996-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}k08901b (FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 11 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase	Barbash, 2011.12.8, k08901 line. k08901 line. [FBrf0217415] Levis, 2003.1.30, l(2)k08901 - two insertions? l(2)k08901 - two insertions? [FBrf0155448] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]