Dmel\P{}lawc^p1a Insertion

General Information
Symbol	Dmel\P{}lawc^p1a	Species	D. melanogaster
Name		FlyBase ID	FBti0025343
Feature type	transposable_element_insertion_site
Description
Inserted element	P-element	Expression data
Affected gene(s)	lawc	Viability / fertility
Causes allele(s)	lawc^p1	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	X ( 7E6-7E7 )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	7E6-7E7 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P-element
Location-dependent role
Size
Associated alleles
Molecular map
Affected Gene(s)
Insertion may affect gene	lawc (Petruk et al., 1998)
Alleles and Phenotypes
Causes alleles	lawc^p1 (Petruk et al., 1998)

Lethality References partially lethal - majority die \| recessive (Brandt and Corces, 2008)
Sterility References
Phenotype Manifest In
	adult thorax (Zorin et al., 1999) adult thorax & macrochaeta (Zorin et al., 1999) arista (Zorin et al., 1995, Simonova et al., 1992, Zorin et al., 1999, Brandt and Corces, 2008) eye (Simonova et al., 1992) leg \| ectopic (Zorin et al., 1999, Brandt and Corces, 2008) macrochaeta (Zorin et al., 1995, Simonova et al., 1992) macrochaeta \| ectopic (Zorin et al., 1999) scutellar bristle (Zorin et al., 1999) scutellum (Zorin et al., 1999) wing (Simonova et al., 1992, Zorin et al., 1999)
Detailed Description
Statement Reference The arista-to-leg transformation seen in lawc[p1] flies is reduced in frequency by 75% if they are also carrying lawc[t6.5]. lawc[t6.5] also rescues the lethality of lawc[p1]. The ability of lawc[p1] to enhance the cut wing phenotype of ct[n] is partially suppressed by lawc[t6.5]; there is a 44% in the frequency of lawc[p1] ct[n] flies with very cut wings if they are also carrying lawc[t6.5]. (Brandt and Corces, 2008) 35-65% of mutant larvae survive to adulthood. Mutants show an arista-to-leg transformation. (Brandt and Corces, 2008) Pros26[unspecified]/+ significantly enhances the arista-to-leg transformation seen in lawc[p1] mutants. (Brandt and Corces, 2008) Phenotype similar to that of the lost alleles scr¹ and bwl¹ with respect to wing and bristle phenotypes, but differs in showing homeotic transformation arista to tarsus not described for scr¹ and bwl¹. (Eizov, 1999.4.10) Does not enhance the frequency of homeotic transformations of trx^B11, brm² or ash1¹⁷ single heterozygotes. The frequency and severity of homeotic transformations (such as haltere to wing or third leg to second leg transformations) in trx^B11 ash1¹⁷ or brm² trx^E2 double heterozygotes is dominantly enhanced by lawc^p1. The enhancement is stronger in a lawc^p1 maternal background. The arista to leg transformation characteristic of lawc^p1 is also enhanced in these flies. lawc^p1/lawc^EF520 females are viable. However, in combination with trx^B11, brm² or ash1¹⁷, lawc^p1/lawc^EF520 results in lethality. lawc^p1/Df(1)RA2 females are viable. However, in combination with trx^B11, brm² or ash1¹⁷, lawc^p1/Df(1)RA2 results in lethality. The ectopic sex combs seen on the second and/or third legs of Df(3L)Pc-MK/+ or Pc⁴/+ males are largely suppressed by lawc^p1. Body colour: the abdominal pigment of y² males is lighter than normal, but uniformly distributed in the last two abdominal segments. y²; mod(mdg4)^ul males have variegated abdominal pigmentation with individual spots of wild-type dark pigment against a lighter pigmented background in the last two abdominal segments. This variegated phenotype is suppressed if the flies are also carrying lawc^p1; the areas of lighter pigmentation increase at the expense of darkly pigmented regions so that the phenotype is closer to y² rather than y² ; mod(mdg4)^ul flies. (Zorin et al., 1999) All lawc^p1 flies show partial transformation of the arista into leg, which is manifested as a thickening of the arista. 1% of flies show complete transformation into leg, such that leg segments and tarsal claws are seen instead of arista tissue. Flies often have ectopic bristles on the scutellum and thorax, and the wings are held apart with multiple incisions in the margins, especially along the posterior margin. The mutant phenotype is stronger in males than females. Most lawc^p1/Df(1)RA2 females die as early larvae. The 10% that do eclose have an enhanced lawc mutant phenotype compared to lawc^p1 flies that includes strong arista to leg transformation, many ectopic bristles and larger wing margin incisions. The 3% of lawc^EF520/lawc^p1 females that eclose have an enhanced lawc mutant phenotype compared to lawc^p1 flies that includes an arista to leg transformation. (Zorin et al., 1999) Temperature sensitive periods for the formation of extra macrochaetae are distributed within larval instars II and III. Different types of macrochaetae differ from one another in their temperature sensitivity. (Simonova et al., 1996) Phenotype is 1% penetrant for complete transformation of arista to leg and fully penetrant for partial transformation, i.e. thickened aristae. Occasionally extra bristles appear on the thorax and scutellum, the wings are hyper-extended with incisions in their margins and the abdominal markings are abnormal. (Zorin et al., 1995) Wings have a ct phenotype, are wide apart and slightly raised. Additional bristles are present on the head, thorax and scutellum. Rough eye phenotype. Transformation of arista in tarsus and distortion of distal leg regions. (Simonova et al., 1992)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	One of two P-element insertions associated with this allele; see P{}lawc^p1b. (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	FBti0014528 (FlyBase, 1992-) P{}lawc^p1a (Petruk et al., 1998, FlyBase, 1992-) P{}P{}lawc^p1 (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 8 )
Research paper	Brandt and Corces, 2008, Mol. Genet. Genomics 280(5): 385--396 The Lawc protein is required for proper transcription by RNA polymerase II in Drosophila. [FBrf0206854] Zorin et al., 1999, Genetics 152(3): 1045--1055 The lawc gene is a new member of the trithorax-group that affects the function of the gypsy insulator of Drosophila. [FBrf0111197] Petruk et al., 1998, Genetika, Moscow 34(3): 446--448 [Cloning of the gene leg-arista-wing complex and analysis of its mutant derivatives in Drosophila.] [FBrf0103009] Simonova et al., 1996, Genetika, Moscow 32(3): 445--447 [Differential characteristics of the temperature-sensitive period of the lawc^p1 mutation on the basis of macrochaetae overexpression in Drosophila.] [FBrf0089827] Simonova et al., 1992, Genetika, Moscow 28(2): 164--167 [Novel regulatory mutation of Drosophila melanogaster.] [FBrf0056276]
Personal communication to FlyBase	Eizov, 1999.4.10, Addition to the FlyBase report. Addition to the FlyBase report. [FBrf0107694]
Abstract	Zorin et al., 1995, A. Dros. Res. Conf. 36: 252B Cloning and characterization of the leg, arista, wing complex gene. [FBrf0079707]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{}lawcp1a Insertion

Dmel\P{}lawc^p1a Insertion