FB2025_01 , released February 20, 2025
Insertion: Dmel\P{lacW}clumsySH1386
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General Information
Symbol
Dmel\P{lacW}clumsySH1386
Species
D. melanogaster
Name
FlyBase ID
FBti0025915
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{lacW}
LINE ID

SH1386

Affected gene(s)
clumsy, l(2)SH1386
Viability / fertility
Associated allele(s)
clumsySH1386, l(2)SH1386SH1386
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
2L ( 39B3 )
Sequence Location
2L:21,209,953..21,209,953 [-]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{lacW}.SH
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2011)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Oh et al., 2003)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:21,209,953..21,209,953 [-]
(Levis and Carlson, 2011.9.12)
Orientation

-

(Levis and Carlson, 2011.9.12)
Cytological location (computed by FlyBase)
39B3 (inferred by FlyBase from sequence location)
Cytological location (reported)
39A1 (reported as inferred from sequence location)
(Oh et al., 2003)
39B3_r5 (reported as inferred from sequence location)
(Levis and Carlson, 2011.9.12)
Insertion into Natural transposon
Comments concerning location

Genomic location previously reported as 2L:21209946..21210355 .

(FlyBase, 2005)
Sequence Data
Inserted Element
Construct
P{lacW}
Size (Kb)

10.691

Component Allele(s)
w+mC
Molecular map
Affected Gene(s)
Insertion may affect gene
clumsy
(Kahsai et al., 2016, FlyBase Curators, 2008-, Oh et al., 2003)
l(2)SH1386
(Oh et al., 2003)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

SH1386

(Levis and Carlson, 2011.9.12)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 1 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Comments
Comments

Deficiency complementation analysis is consistent with the lethality of the line being associated with the insertion. The insertion maps within the clumsy transcription unit. However, since the insertion lies within a gene-rich region, the lethality can not yet be conclusively mapped to a single gene from these results. Thus l(2)SH1386SH1386 (representing the lethality of the chromosome) has been kept as a separate allele in FlyBase for now.

(Kahsai et al., 2016)

Location 2L:21197538-21197949 confirmed by FlyBase alignment of dbGSS accession AB082477 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.

(FlyBase, 2005)
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{lacW}clumsySH1386
(Kahsai et al., 2016)
P{lacW}l(2)SH1386SH1386
(FlyBase, 2005, Oh et al., 2003, FlyBase, 1992-)
SH1386
(Levis and Carlson, 2011.9.12)
Name Synonyms
Secondary FlyBase IDs
    References (7)