Dmel\P{lacW}Bsg^SH1217 Insertion

General Information
Symbol	Dmel\P{lacW}Bsg^SH1217	Species	D. melanogaster
Name		FlyBase ID	FBti0025956
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Bsg	Viability / fertility
Causes allele(s)	Bsg¹²¹⁷	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	2L ( 28E4 )	Sequence location	2L:8,088,391..8,088,511
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{lacW}.SH A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800). Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Oh et al., 2003, Bellen et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:8,088,391..8,088,511 (FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)	28E4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	28E4-28E5 (reported as inferred from sequence location) (Oh et al., 2003)
Comments concerning location
Sequence Data
Flanking sequence	AB082425 (FlyBase, 1992-, Oh et al., 2003)
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Bsg (Oh et al., 2003, Besse et al., 2007)
Alleles and Phenotypes
Causes alleles	Bsg¹²¹⁷ (Oh et al., 2003, Besse et al., 2007)

Lethality References lethal \| recessive (Oh et al., 2003) partially lethal - majority die \| larval stage (Besse et al., 2007) partially lethal - majority die \| late embryonic stage (Besse et al., 2007)
Sterility References
Phenotype Manifest In
	NMJ bouton (Besse et al., 2007)
Detailed Description
Statement Reference Bsg[1217]/Df(2L)Exel7034 hemizygous flies are semi-lethal, with approximately 50% reaching third larval instar. Axonal targeting is not altered to a visible degree in Bsg[1217]/Df(2L)Exel7034 transheterozygotes. The proportion of very large boutons is greatly increased compared to controls. The observed increase in bouton size is associated with a concomitant reduction of both NMJ branching and bouton number, keeping the overall NMJ size close to normal. (Besse et al., 2007)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	Location 2L:8088390-8088512 confirmed by FlyBase alignment of dbGSS accession AB082425 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	l'^2'SH1217^SH1217 (Besse et al., 2007) P{lacW}Bsg^SH1217 P{lacW}l(2)SH1217^SH1217 (Oh et al., 2003, FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 8 )
Research paper	Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Besse et al., 2007, J. Cell Biol. 177(5): 843--855 The Ig cell adhesion molecule Basigin controls compartmentalization and vesicle release at Drosophila melanogaster synapses. [FBrf0202422] Oh et al., 2003, Genetics 163(1): 195--201 A P-element insertion screen identified mutations in 455 novel essential genes in Drosophila. [FBrf0155812] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Oh et al., 2003, Genetics 163(1): Table S1. [FBrf0157097]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacW}BsgSH1217 Insertion

Dmel\P{lacW}Bsg^SH1217 Insertion