Dmel\P{lacW}bsf^SH1181 Insertion

General Information
Symbol	Dmel\P{lacW}bsf^SH1181	Species	D. melanogaster
Name		FlyBase ID	FBti0025967
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	bsf, l(2)SH1181	Viability / fertility
Causes allele(s)	bsf^SH1181, l(2)SH1181^SH1181	Stock availability	1 publicly available
LINE ID	SH1181
Genomic Location
Chromosomal location	2L ( 36F5 )	Sequence location	2L:18,452,250..18,452,250 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{lacW}.SH A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800). Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Oh et al., 2003, Bellen et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:18,452,250..18,452,250 [-] (Levis and Carlson, 2011.9.12)
Orientation	- (Levis and Carlson, 2011.9.12)
Cytological location (computed by FlyBase)	36F5 ( inferred by FlyBase from sequence location )
Cytological location (reported)	36F5_r5 (reported as inferred from sequence location) (Levis and Carlson, 2011.9.12) 36F7 (reported as inferred from sequence location) (Oh et al., 2003)
Comments concerning location	Genomic location previously reported as 2L:18452243..18452287. (FlyBase, 2005)
Sequence Data
Flanking sequence	AB082414 (Oh et al., 2003, FlyBase, 1992-) GS635296 (Levis and Carlson, 2011.9.12)
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	bsf (Oh et al., 2003, Link et al., 2007, FlyBase Curators, 2008) l(2)SH1181 (Oh et al., 2003, Geng and MacDonald, 2007)
Alleles and Phenotypes
Causes alleles	bsf^SH1181 (Link et al., 2007, FlyBase Curators, 2008) l(2)SH1181^SH1181 (Oh et al., 2003, Geng and MacDonald, 2007)

Lethality References lethal \| recessive (Oh et al., 2003)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	SH1181 (Levis and Carlson, 2011.9.12)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	29492 w^*; P{lacW}bsf^SH1181 P{neoFRT}40A, l(2)SH1181^SH1181/CyO
Linkouts
Comments
	Location 2L:18448171-18448217 confirmed by FlyBase alignment of dbGSS accession AB082414 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	l(2)SH1181-FRT40 (Link et al., 2007) P{lacW}bsf^SH1181 P{lacW}l(2)SH1181^SH1181 (Oh et al., 2003, FlyBase, 2005, FlyBase, 1992-) SH1181 (Geng and MacDonald, 2007, Levis and Carlson, 2011.9.12)
Secondary FlyBase IDs

References ( 11 )
Research paper	Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Geng and MacDonald, 2007, Fly 1(5): 259--267 Identification of genes that influence gurken expression. [FBrf0205043] Oh et al., 2003, Genetics 163(1): 195--201 A P-element insertion screen identified mutations in 455 novel essential genes in Drosophila. [FBrf0155812] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Link et al., 2007, J. Cell Biol. 178(4): Link et al., 2007. Supplemental Table 2. [FBrf0218017] Oh et al., 2003, Genetics 163(1): Table S1. [FBrf0157097]
Personal communication to FlyBase	Levis and Carlson, 2011.9.12, Updated location data for SH lines Updated location data for SH lines [FBrf0215563]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacW}bsfSH1181 Insertion

Dmel\P{lacW}bsf^SH1181 Insertion